Efficient nursery plant production of dwarf cogongrass (lmperata cylindrica L.) through mass propagation in liquid culture
Dwarf cogongrass (Imperata cylindrica L.) was developed as a dwarf mutant through hear,y-ion beam irradiation 7 years ago. The dwarf mutant could be expected to use as a new variety for a cover plant with low maintenance, although it has poor seed fertility. To establish an effrcient nursery prod...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Grassland Science
2012
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/135611/1/Journal%20Grassland%20Science_Efficient%20nursery%20plant%20production%20of%20dwarf%20cogongrass.pdf |
| _version_ | 1797036414306615296 |
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| author | Nafiatul Umami, N. Umami Takahiro Gondo, T. Gondo Hidenori Tanaka, H. Tanaka Mohammad M. Rahman, M. M. Rahman Ryo Akashi, R. Akashi |
| author_facet | Nafiatul Umami, N. Umami Takahiro Gondo, T. Gondo Hidenori Tanaka, H. Tanaka Mohammad M. Rahman, M. M. Rahman Ryo Akashi, R. Akashi |
| author_sort | Nafiatul Umami, N. Umami |
| collection | UGM |
| description | Dwarf cogongrass (Imperata cylindrica L.) was developed as a dwarf mutant
through hear,y-ion beam irradiation 7 years ago. The dwarf mutant could be
expected to use as a new variety for a cover plant with low maintenance,
although it has poor seed fertility. To establish an effrcient nursery production
system for dwarf cogongrass, we attempted mass propagation of it in liquid
culture and investigated the genetic stabitity of its regenerants. Multiple-shoot
clumps (MSCs) were initiated from apical meristems of dwarf cogongrass on
Murashige and Skoog (MS) solid medium supplemented with 0.1 mgL-t 2,4-
dichlorophenoxyacetic acid (2,4-D) and 2.0 mg L t benzylaminopurine (BAp).
Mass propagation conditions were established from MSCs cultured in MS
Iiquid medium containing 0.05 mg L-l 2,4-D and 0.5 mg L 1 BAp. The fresh
weight of the MSCs per flask increased by more than 16 times in 14 days of
liquid culture. Two different sizes of MSCs were produced in liquid curture.
when smaller MSCs (<2 mm in diameter) were transferred to half-strength
hormone-free MS solid medium, plant regeneration occurred at high fiequency
(93.3vo). These tissues showed high regenerative potential with approximately
350 green shoots recovered within 50 days from 60 regenerating clumps. Furthermore,
root elongation was vigorous in the regenerants growing in the same
medium. Regenerated plants were acclimatized in hydrated )iS,-7 pellets for
30 days and then grown in soil as nursery plants. The plant height of regenerants
was almost the same as original dwarf cogongrass and significantly lower
than the wild qpe plants (P < 0.01). Analysis of amplified fragment length
polymorphism (AFLP) banding patterns generated using 10 primer combinations
showed no major genetic variations among the regenerated plants and
original dwarf cogongrass. |
| first_indexed | 2024-03-13T23:45:28Z |
| format | Article |
| id | oai:generic.eprints.org:135611 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-13T23:45:28Z |
| publishDate | 2012 |
| publisher | Grassland Science |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1356112016-01-20T02:31:12Z https://repository.ugm.ac.id/135611/ Efficient nursery plant production of dwarf cogongrass (lmperata cylindrica L.) through mass propagation in liquid culture Nafiatul Umami, N. Umami Takahiro Gondo, T. Gondo Hidenori Tanaka, H. Tanaka Mohammad M. Rahman, M. M. Rahman Ryo Akashi, R. Akashi Agricultural Land Management Agronomy Husbandry Dwarf cogongrass (Imperata cylindrica L.) was developed as a dwarf mutant through hear,y-ion beam irradiation 7 years ago. The dwarf mutant could be expected to use as a new variety for a cover plant with low maintenance, although it has poor seed fertility. To establish an effrcient nursery production system for dwarf cogongrass, we attempted mass propagation of it in liquid culture and investigated the genetic stabitity of its regenerants. Multiple-shoot clumps (MSCs) were initiated from apical meristems of dwarf cogongrass on Murashige and Skoog (MS) solid medium supplemented with 0.1 mgL-t 2,4- dichlorophenoxyacetic acid (2,4-D) and 2.0 mg L t benzylaminopurine (BAp). Mass propagation conditions were established from MSCs cultured in MS Iiquid medium containing 0.05 mg L-l 2,4-D and 0.5 mg L 1 BAp. The fresh weight of the MSCs per flask increased by more than 16 times in 14 days of liquid culture. Two different sizes of MSCs were produced in liquid curture. when smaller MSCs (<2 mm in diameter) were transferred to half-strength hormone-free MS solid medium, plant regeneration occurred at high fiequency (93.3vo). These tissues showed high regenerative potential with approximately 350 green shoots recovered within 50 days from 60 regenerating clumps. Furthermore, root elongation was vigorous in the regenerants growing in the same medium. Regenerated plants were acclimatized in hydrated )iS,-7 pellets for 30 days and then grown in soil as nursery plants. The plant height of regenerants was almost the same as original dwarf cogongrass and significantly lower than the wild qpe plants (P < 0.01). Analysis of amplified fragment length polymorphism (AFLP) banding patterns generated using 10 primer combinations showed no major genetic variations among the regenerated plants and original dwarf cogongrass. Grassland Science 2012 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/135611/1/Journal%20Grassland%20Science_Efficient%20nursery%20plant%20production%20of%20dwarf%20cogongrass.pdf Nafiatul Umami, N. Umami and Takahiro Gondo, T. Gondo and Hidenori Tanaka, H. Tanaka and Mohammad M. Rahman, M. M. Rahman and Ryo Akashi, R. Akashi (2012) Efficient nursery plant production of dwarf cogongrass (lmperata cylindrica L.) through mass propagation in liquid culture. Grassland Science, 58. pp. 201-207. ISSN 1744-6961 http://www.readcube.com/articles/10.1111%2Fgrs.12001?r3_referer=wol&tracking_action=preview_click&show_checkout=1&purchase_referrer=onlinelibrary.wiley.com&purchase_site_license=LICENSE_DENIED |
| spellingShingle | Agricultural Land Management Agronomy Husbandry Nafiatul Umami, N. Umami Takahiro Gondo, T. Gondo Hidenori Tanaka, H. Tanaka Mohammad M. Rahman, M. M. Rahman Ryo Akashi, R. Akashi Efficient nursery plant production of dwarf cogongrass (lmperata cylindrica L.) through mass propagation in liquid culture |
| title | Efficient nursery plant production of dwarf cogongrass
(lmperata cylindrica L.) through mass propagation in liquid
culture |
| title_full | Efficient nursery plant production of dwarf cogongrass
(lmperata cylindrica L.) through mass propagation in liquid
culture |
| title_fullStr | Efficient nursery plant production of dwarf cogongrass
(lmperata cylindrica L.) through mass propagation in liquid
culture |
| title_full_unstemmed | Efficient nursery plant production of dwarf cogongrass
(lmperata cylindrica L.) through mass propagation in liquid
culture |
| title_short | Efficient nursery plant production of dwarf cogongrass
(lmperata cylindrica L.) through mass propagation in liquid
culture |
| title_sort | efficient nursery plant production of dwarf cogongrass lmperata cylindrica l through mass propagation in liquid culture |
| topic | Agricultural Land Management Agronomy Husbandry |
| url | https://repository.ugm.ac.id/135611/1/Journal%20Grassland%20Science_Efficient%20nursery%20plant%20production%20of%20dwarf%20cogongrass.pdf |
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