| Summary: | The aim of this study was to evaluate of genetic diversity
of local cattle in Indonesia. We analyzed the
polymorphism of mtDNA cytochrome b (cytb) gene using
Polymerase Chain Reaction-Restriction Fragment Length
Polymorphism (PCR-RFLP) method. Seventy-three
Indonesian local cattle were used in the study that
consisted of Madura cattle from Madura island (n = 15),
Madura cattle from Sapudi island (n = 23), Limousin x
Madura from Madura island (n = 5), Bali cattle from
Kupang (n = 12), Aceh cattle from Aceh (n = 5), Pesisir
cattle (n = 4), Bali cattle from West Sumatera (n = 1), and
Java cattle from Pacitan (n = 8). The 464-bp fragment of
cytochrome b (cyt b) gene was amplified using primers:
forward primer of L14735 and reverse primer of H15149.
The PCR product was digested using HinfI and TaqI
restriction enzyme to identify the polymorphism of
mtDNA cytochrome b (cytb) gene. The result showed
that there was two haplotypes (A and B). Aceh and Pesisir
cattle have 100% haplotype B meanwhile Bali cattle (from
Kupang and West Sumatera) and Limura cattle have 100%
haplotype A. Whereas, Madura cattle from Madura and
Sapudi island and Java cattle have two different
haplotypes (A and B). There was mt-DNA cytochrome b
polymorphism of local cattle in Indonesia based on PCRRFLP
analysis method. Since the genetic variation in local
cattle showed the differences in the specific area, it is
necessary to control the cattle populations based on the
species.
Keywords: Polymorphism; genetic variation; cytochrome
b gene; local cattle; Indonesia
* Corresponding author. Tel.:+62-
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