Influence of Cellulolytic Bacteria from Rumen Fluid on in Vitro Gas Production of Fermented Robusta Coffee Pulp (Coffea canephora sp.)
ABSTRACT: The experiment was done to evaluate the influence of robusta coffee pulp fermented by rumen cellulolytic bacteria on in vitro gas production. The first step of this experiment was cellulolytic inoculum production by using fluid fermentation with cellulose as substrat. The inoculum produced...
| Main Authors: | , , |
|---|---|
| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2015
|
| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/136005/1/Proceeding%20ISTAP%202015%20NR-19-O.%20LMY%2C%20C.%20Hanim.pdf |
| _version_ | 1797036470699032576 |
|---|---|
| author | Hanim, Chusnul Yusiati, Lies Mira Jazim, Fahriza Anjaya |
| author_facet | Hanim, Chusnul Yusiati, Lies Mira Jazim, Fahriza Anjaya |
| author_sort | Hanim, Chusnul |
| collection | UGM |
| description | ABSTRACT: The experiment was done to evaluate the influence of robusta coffee pulp fermented by rumen cellulolytic bacteria on in vitro gas production. The first step of this experiment was cellulolytic inoculum production by using fluid fermentation with cellulose as substrat. The inoculum produced was then used for coffee pulp fermentation. Cellulolytic bacteria was added into 200 g coffee pulp as much as 0%, 5% and 10% based on dry matter. Each treatment had three replicates. Fermentation was carried out at room temperature during 21 days anaerobically. At the end of ermentation, samples were taken out for nutrient determination including physical, chemical qualities, and in vitro gas production. Data obtained were analyzed by one way design and continued by Duncan’s new multiple range test to examine the differences between mean values. The results showed that 5% and 10% cellulolytic bacteria addition decreased pH value, and crude fiber (CF) content as much as 12.89% and 16.32% compared to control (0% of cellulolytic bacteria). Whereas addition of 10% inoculum increased nitrogen free extract (NFE) content. However, cellulolytic bacteria addition up to 10% had no effect on crude protein (CP), extract ether (EE), dry matter (DM), organic matter (OM), glucose, and lactic acid content, as well as in vitro gas production. It could be concluded that cellulolytic bacteria addition in level 5% decreased
CF content but did not give positive effect on in vitro gas production.
Keyswords: Cellulolytic Bacteria, Chemical Composition, Coffee Pulp, In Vitro Gas
Production |
| first_indexed | 2024-03-13T23:46:57Z |
| format | Conference or Workshop Item |
| id | oai:generic.eprints.org:136005 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-13T23:46:57Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1360052016-03-03T07:41:57Z https://repository.ugm.ac.id/136005/ Influence of Cellulolytic Bacteria from Rumen Fluid on in Vitro Gas Production of Fermented Robusta Coffee Pulp (Coffea canephora sp.) Hanim, Chusnul Yusiati, Lies Mira Jazim, Fahriza Anjaya Animal Production Fermentation ABSTRACT: The experiment was done to evaluate the influence of robusta coffee pulp fermented by rumen cellulolytic bacteria on in vitro gas production. The first step of this experiment was cellulolytic inoculum production by using fluid fermentation with cellulose as substrat. The inoculum produced was then used for coffee pulp fermentation. Cellulolytic bacteria was added into 200 g coffee pulp as much as 0%, 5% and 10% based on dry matter. Each treatment had three replicates. Fermentation was carried out at room temperature during 21 days anaerobically. At the end of ermentation, samples were taken out for nutrient determination including physical, chemical qualities, and in vitro gas production. Data obtained were analyzed by one way design and continued by Duncan’s new multiple range test to examine the differences between mean values. The results showed that 5% and 10% cellulolytic bacteria addition decreased pH value, and crude fiber (CF) content as much as 12.89% and 16.32% compared to control (0% of cellulolytic bacteria). Whereas addition of 10% inoculum increased nitrogen free extract (NFE) content. However, cellulolytic bacteria addition up to 10% had no effect on crude protein (CP), extract ether (EE), dry matter (DM), organic matter (OM), glucose, and lactic acid content, as well as in vitro gas production. It could be concluded that cellulolytic bacteria addition in level 5% decreased CF content but did not give positive effect on in vitro gas production. Keyswords: Cellulolytic Bacteria, Chemical Composition, Coffee Pulp, In Vitro Gas Production 2015-10-20 Conference or Workshop Item PeerReviewed application/pdf en https://repository.ugm.ac.id/136005/1/Proceeding%20ISTAP%202015%20NR-19-O.%20LMY%2C%20C.%20Hanim.pdf Hanim, Chusnul and Yusiati, Lies Mira and Jazim, Fahriza Anjaya (2015) Influence of Cellulolytic Bacteria from Rumen Fluid on in Vitro Gas Production of Fermented Robusta Coffee Pulp (Coffea canephora sp.). In: The 6th International Seminar on Tropical Animal Production, October 20-22, 2015, Faculty of Animal Science, Universitas Gadjah Mada Yogyakarta, Indonesia. |
| spellingShingle | Animal Production Fermentation Hanim, Chusnul Yusiati, Lies Mira Jazim, Fahriza Anjaya Influence of Cellulolytic Bacteria from Rumen Fluid on in Vitro Gas Production of Fermented Robusta Coffee Pulp (Coffea canephora sp.) |
| title | Influence of Cellulolytic Bacteria from Rumen Fluid on in Vitro Gas Production of Fermented Robusta Coffee Pulp (Coffea canephora sp.) |
| title_full | Influence of Cellulolytic Bacteria from Rumen Fluid on in Vitro Gas Production of Fermented Robusta Coffee Pulp (Coffea canephora sp.) |
| title_fullStr | Influence of Cellulolytic Bacteria from Rumen Fluid on in Vitro Gas Production of Fermented Robusta Coffee Pulp (Coffea canephora sp.) |
| title_full_unstemmed | Influence of Cellulolytic Bacteria from Rumen Fluid on in Vitro Gas Production of Fermented Robusta Coffee Pulp (Coffea canephora sp.) |
| title_short | Influence of Cellulolytic Bacteria from Rumen Fluid on in Vitro Gas Production of Fermented Robusta Coffee Pulp (Coffea canephora sp.) |
| title_sort | influence of cellulolytic bacteria from rumen fluid on in vitro gas production of fermented robusta coffee pulp coffea canephora sp |
| topic | Animal Production Fermentation |
| url | https://repository.ugm.ac.id/136005/1/Proceeding%20ISTAP%202015%20NR-19-O.%20LMY%2C%20C.%20Hanim.pdf |
| work_keys_str_mv | AT hanimchusnul influenceofcellulolyticbacteriafromrumenfluidoninvitrogasproductionoffermentedrobustacoffeepulpcoffeacanephorasp AT yusiatiliesmira influenceofcellulolyticbacteriafromrumenfluidoninvitrogasproductionoffermentedrobustacoffeepulpcoffeacanephorasp AT jazimfahrizaanjaya influenceofcellulolyticbacteriafromrumenfluidoninvitrogasproductionoffermentedrobustacoffeepulpcoffeacanephorasp |