Selection of Phalaenopsis amabilis (L.) Blume Orchid Resistance to Hygromycin
Examination of Phalaenopsis amabilis orchid resistance to hygromycin antibiotic is an important step to do prior to Agrobacterium-mediated genetic transformation in this orchids using Hygromycin phosphotransferase (HPT) gene as a selection marker in the T-DNA that harboring a desired gene to be tran...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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2012
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| Online Access: | https://repository.ugm.ac.id/136029/4/B%2043%20IJB%202013-Mercuriani%20et%20al.pdf |
| _version_ | 1826049464438095872 |
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| author | Mercuriani, Ixora Purwantoro, Aziz Moeljopawiro, Sukarti Jang, Seonghoe Semiarti, Endang |
| author_facet | Mercuriani, Ixora Purwantoro, Aziz Moeljopawiro, Sukarti Jang, Seonghoe Semiarti, Endang |
| author_sort | Mercuriani, Ixora |
| collection | UGM |
| description | Examination of Phalaenopsis amabilis orchid resistance to hygromycin antibiotic is an important step to do prior to Agrobacterium-mediated genetic transformation in this orchids using Hygromycin phosphotransferase (HPT) gene as a selection marker in the T-DNA that harboring a desired gene to be transfered. We exposed the plant on hygromycin containing medium. The experiment was conducted using 6 weeks old P. amabilis protocorms. These protocorms were subcultured onto NP medium supplemented with various concentration of Hygromycin (0, 5, 10, 20, 1nd 40 mg.L-1). The number of survival protocorms were examined every week for 4 weeks after subcultured (WAS). The resistancy of hygromycin was calculated as ratio of death protocorms per total protocorms). The result showed that 10 mg.L-1 hygromycin with 1 weeks of application caused death close to LD 50. This data indicate that P. amabilis resistance to hygromycin treatment on the appropriate concentration 10 mg.L-1, and this concentration can be used for other purposes in orchid system |
| first_indexed | 2024-03-13T23:46:59Z |
| format | Article |
| id | oai:generic.eprints.org:136029 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-13T23:46:59Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1360292016-03-07T03:49:25Z https://repository.ugm.ac.id/136029/ Selection of Phalaenopsis amabilis (L.) Blume Orchid Resistance to Hygromycin Mercuriani, Ixora Purwantoro, Aziz Moeljopawiro, Sukarti Jang, Seonghoe Semiarti, Endang Agricultural Biotechnology Diagnostics (incl. Biosensors) Environmental Biotechnology Diagnostics (incl. Biosensors) Examination of Phalaenopsis amabilis orchid resistance to hygromycin antibiotic is an important step to do prior to Agrobacterium-mediated genetic transformation in this orchids using Hygromycin phosphotransferase (HPT) gene as a selection marker in the T-DNA that harboring a desired gene to be transfered. We exposed the plant on hygromycin containing medium. The experiment was conducted using 6 weeks old P. amabilis protocorms. These protocorms were subcultured onto NP medium supplemented with various concentration of Hygromycin (0, 5, 10, 20, 1nd 40 mg.L-1). The number of survival protocorms were examined every week for 4 weeks after subcultured (WAS). The resistancy of hygromycin was calculated as ratio of death protocorms per total protocorms). The result showed that 10 mg.L-1 hygromycin with 1 weeks of application caused death close to LD 50. This data indicate that P. amabilis resistance to hygromycin treatment on the appropriate concentration 10 mg.L-1, and this concentration can be used for other purposes in orchid system 2012-12 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/136029/4/B%2043%20IJB%202013-Mercuriani%20et%20al.pdf Mercuriani, Ixora and Purwantoro, Aziz and Moeljopawiro, Sukarti and Jang, Seonghoe and Semiarti, Endang (2012) Selection of Phalaenopsis amabilis (L.) Blume Orchid Resistance to Hygromycin. Indonesian Journal of Biotechnology, 17 (2). pp. 107-113. ISSN 2089-2241 http://ijbiotech.ugm.ac.id |
| spellingShingle | Agricultural Biotechnology Diagnostics (incl. Biosensors) Environmental Biotechnology Diagnostics (incl. Biosensors) Mercuriani, Ixora Purwantoro, Aziz Moeljopawiro, Sukarti Jang, Seonghoe Semiarti, Endang Selection of Phalaenopsis amabilis (L.) Blume Orchid Resistance to Hygromycin |
| title | Selection of Phalaenopsis amabilis (L.) Blume Orchid Resistance to Hygromycin |
| title_full | Selection of Phalaenopsis amabilis (L.) Blume Orchid Resistance to Hygromycin |
| title_fullStr | Selection of Phalaenopsis amabilis (L.) Blume Orchid Resistance to Hygromycin |
| title_full_unstemmed | Selection of Phalaenopsis amabilis (L.) Blume Orchid Resistance to Hygromycin |
| title_short | Selection of Phalaenopsis amabilis (L.) Blume Orchid Resistance to Hygromycin |
| title_sort | selection of phalaenopsis amabilis l blume orchid resistance to hygromycin |
| topic | Agricultural Biotechnology Diagnostics (incl. Biosensors) Environmental Biotechnology Diagnostics (incl. Biosensors) |
| url | https://repository.ugm.ac.id/136029/4/B%2043%20IJB%202013-Mercuriani%20et%20al.pdf |
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