The Detailed Motility and Velocity Characteristics of Rams Spermatozoa as Assessed by Computer-Aided Semen Analysis

ABSTRACT: The aim of the present study was to determine the effect of incubation time in TALP medium on the detailed motility and velocity of spermatozoa characteristics. Four rams (R9, R12, R13, R16) of proven fertility were used in this study. Semen was collected by electroejaculation. Rams semen...

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Main Author: Ismaya, Ismaya
Format: Conference or Workshop Item
Language:English
Published: 2015
Subjects:
Online Access:https://repository.ugm.ac.id/136459/7/The_6th_ISTAP%20P%202015%20%28Ismaya-28%29.pdf
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author Ismaya, Ismaya
author_facet Ismaya, Ismaya
author_sort Ismaya, Ismaya
collection UGM
description ABSTRACT: The aim of the present study was to determine the effect of incubation time in TALP medium on the detailed motility and velocity of spermatozoa characteristics. Four rams (R9, R12, R13, R16) of proven fertility were used in this study. Semen was collected by electroejaculation. Rams semen was collected with 4 replicates, respectively to analyzed the motility and velocity characteristics by using the computer-aided semen analysis (CASA). Data were analyzed using SPSS software program. The results showed that percentage of motile, progressive and rapid were significantly decreased from 0 to 120 minutes incubation. In contrast, percentage of static was significantly increased from 21.6% to 71.3% at 0 to 120 minutes incubation. Ram 12 had the best motile with 76.3% at 0 minute incubation and 40.3% at 120 minutes incubation. Average path velocity (VAP), progressive velocity (VSL) and track speed (VCL) at 0 incubation were 91.2 pm/s, 72.2 pm/s and 134 pm/s afterward decreased to 49 pm/s, 40.8 um/s and 77.7 pm/s at 120 minutes incubation, respectively. In conclusion, rams and incubation time were influenced the motility and velocity of spermatozoa. Keywords: Motility, Velocity, Rams Spermatozoa.
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spelling oai:generic.eprints.org:1364592016-08-19T00:34:13Z https://repository.ugm.ac.id/136459/ The Detailed Motility and Velocity Characteristics of Rams Spermatozoa as Assessed by Computer-Aided Semen Analysis Ismaya, Ismaya Animal Reproduction ABSTRACT: The aim of the present study was to determine the effect of incubation time in TALP medium on the detailed motility and velocity of spermatozoa characteristics. Four rams (R9, R12, R13, R16) of proven fertility were used in this study. Semen was collected by electroejaculation. Rams semen was collected with 4 replicates, respectively to analyzed the motility and velocity characteristics by using the computer-aided semen analysis (CASA). Data were analyzed using SPSS software program. The results showed that percentage of motile, progressive and rapid were significantly decreased from 0 to 120 minutes incubation. In contrast, percentage of static was significantly increased from 21.6% to 71.3% at 0 to 120 minutes incubation. Ram 12 had the best motile with 76.3% at 0 minute incubation and 40.3% at 120 minutes incubation. Average path velocity (VAP), progressive velocity (VSL) and track speed (VCL) at 0 incubation were 91.2 pm/s, 72.2 pm/s and 134 pm/s afterward decreased to 49 pm/s, 40.8 um/s and 77.7 pm/s at 120 minutes incubation, respectively. In conclusion, rams and incubation time were influenced the motility and velocity of spermatozoa. Keywords: Motility, Velocity, Rams Spermatozoa. 2015-10-20 Conference or Workshop Item PeerReviewed application/pdf en https://repository.ugm.ac.id/136459/7/The_6th_ISTAP%20P%202015%20%28Ismaya-28%29.pdf Ismaya, Ismaya (2015) The Detailed Motility and Velocity Characteristics of Rams Spermatozoa as Assessed by Computer-Aided Semen Analysis. In: Integrated Approach in Developing Sustainable Tropical Animal Production, october 20-22, 2015, Yogyakarta.
spellingShingle Animal Reproduction
Ismaya, Ismaya
The Detailed Motility and Velocity Characteristics of Rams Spermatozoa as Assessed by Computer-Aided Semen Analysis
title The Detailed Motility and Velocity Characteristics of Rams Spermatozoa as Assessed by Computer-Aided Semen Analysis
title_full The Detailed Motility and Velocity Characteristics of Rams Spermatozoa as Assessed by Computer-Aided Semen Analysis
title_fullStr The Detailed Motility and Velocity Characteristics of Rams Spermatozoa as Assessed by Computer-Aided Semen Analysis
title_full_unstemmed The Detailed Motility and Velocity Characteristics of Rams Spermatozoa as Assessed by Computer-Aided Semen Analysis
title_short The Detailed Motility and Velocity Characteristics of Rams Spermatozoa as Assessed by Computer-Aided Semen Analysis
title_sort detailed motility and velocity characteristics of rams spermatozoa as assessed by computer aided semen analysis
topic Animal Reproduction
url https://repository.ugm.ac.id/136459/7/The_6th_ISTAP%20P%202015%20%28Ismaya-28%29.pdf
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