359-K230JP. Symbiotic Nitrogen-Fixing Soil Bacterium Has an Ability of Methanol Utilization Depending on Rare Earth Elements
Bradyrhizobium genus is one of bacterial soil fertilizers in agricultural system. In this study, we showed that the most popular soil fertilizer, B. japonicum strain USDA110, has an ability to utilize methanol depending on Ce3+, which is one of the rare earth elements. In strain USDA110, methanol de...
| Main Authors: | , , , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
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The Asian-Australasian Association of Animal Production Societies
2014
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/136549/1/The_16th_AAAP%20ALL.pdf |
| _version_ | 1826049525793423360 |
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| author | Kurniawati, Novita Mitsui, Ryoji Tani, Akio Fitriyanto, Nanung Agus Pertiwiningrum, Ambar Hayakawa, Takashi Nakagawa, Tomoyuki Kawai, Keiichi |
| author_facet | Kurniawati, Novita Mitsui, Ryoji Tani, Akio Fitriyanto, Nanung Agus Pertiwiningrum, Ambar Hayakawa, Takashi Nakagawa, Tomoyuki Kawai, Keiichi |
| author_sort | Kurniawati, Novita |
| collection | UGM |
| description | Bradyrhizobium genus is one of bacterial soil fertilizers in agricultural system. In this study, we showed that the most popular soil fertilizer, B. japonicum strain USDA110, has an ability to utilize methanol depending on Ce3+, which is one of the rare earth elements. In strain USDA110, methanol dehydrogenase (MDH) activity was induced by presence of Ce3+, although it was reported that the strain did not show enough MDH activity depending on Ca2+. Moreover, we purified MDH from the strain grown on methanol/Ce3+ medium. Purified MDH showed single band on SDS-PAGE, and its specific activity was 2.69 U/mg. Its optimum pH and temperature were 9.0 and 35ºC, respectively, and the Km value with methanol was calculated to be 0.423 mM. From these results, it is suggested that rare earth elements, especially Ce3+, is one of the important factors for the methanol utilization of B. japonicum strain USDA110, and Ce3+ functions as inducer/cofactor for MDH encoded by xoxF gene. |
| first_indexed | 2024-03-13T23:47:56Z |
| format | Conference or Workshop Item |
| id | oai:generic.eprints.org:136549 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-13T23:47:56Z |
| publishDate | 2014 |
| publisher | The Asian-Australasian Association of Animal Production Societies |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1365492016-08-22T08:38:14Z https://repository.ugm.ac.id/136549/ 359-K230JP. Symbiotic Nitrogen-Fixing Soil Bacterium Has an Ability of Methanol Utilization Depending on Rare Earth Elements Kurniawati, Novita Mitsui, Ryoji Tani, Akio Fitriyanto, Nanung Agus Pertiwiningrum, Ambar Hayakawa, Takashi Nakagawa, Tomoyuki Kawai, Keiichi Agricultural Spatial Analysis and Modelling Agricultural System Analysis and Modelling Sustainable Agricultural Development Farm Management Rural Management Agribusiness Agricultural Management (Others) Animal Production Crop and Pasture Production Other Agricultural and Veterinary Sciences Agricultural Land Management Biological Sciences Food technology Husbandry Livestock Veterinary Sciences Bradyrhizobium genus is one of bacterial soil fertilizers in agricultural system. In this study, we showed that the most popular soil fertilizer, B. japonicum strain USDA110, has an ability to utilize methanol depending on Ce3+, which is one of the rare earth elements. In strain USDA110, methanol dehydrogenase (MDH) activity was induced by presence of Ce3+, although it was reported that the strain did not show enough MDH activity depending on Ca2+. Moreover, we purified MDH from the strain grown on methanol/Ce3+ medium. Purified MDH showed single band on SDS-PAGE, and its specific activity was 2.69 U/mg. Its optimum pH and temperature were 9.0 and 35ºC, respectively, and the Km value with methanol was calculated to be 0.423 mM. From these results, it is suggested that rare earth elements, especially Ce3+, is one of the important factors for the methanol utilization of B. japonicum strain USDA110, and Ce3+ functions as inducer/cofactor for MDH encoded by xoxF gene. The Asian-Australasian Association of Animal Production Societies 2014-11-10 Conference or Workshop Item PeerReviewed application/pdf en https://repository.ugm.ac.id/136549/1/The_16th_AAAP%20ALL.pdf Kurniawati, Novita and Mitsui, Ryoji and Tani, Akio and Fitriyanto, Nanung Agus and Pertiwiningrum, Ambar and Hayakawa, Takashi and Nakagawa, Tomoyuki and Kawai, Keiichi (2014) 359-K230JP. Symbiotic Nitrogen-Fixing Soil Bacterium Has an Ability of Methanol Utilization Depending on Rare Earth Elements. In: The 16th Asian-Australasian Association of Aninal Production Societies Congress, 10-14 November 2014, Universitas Gadjah Mada. http://www.ajas.info/aaap/about.php |
| spellingShingle | Agricultural Spatial Analysis and Modelling Agricultural System Analysis and Modelling Sustainable Agricultural Development Farm Management Rural Management Agribusiness Agricultural Management (Others) Animal Production Crop and Pasture Production Other Agricultural and Veterinary Sciences Agricultural Land Management Biological Sciences Food technology Husbandry Livestock Veterinary Sciences Kurniawati, Novita Mitsui, Ryoji Tani, Akio Fitriyanto, Nanung Agus Pertiwiningrum, Ambar Hayakawa, Takashi Nakagawa, Tomoyuki Kawai, Keiichi 359-K230JP. Symbiotic Nitrogen-Fixing Soil Bacterium Has an Ability of Methanol Utilization Depending on Rare Earth Elements |
| title | 359-K230JP. Symbiotic Nitrogen-Fixing Soil Bacterium Has an Ability of Methanol Utilization Depending on Rare Earth Elements |
| title_full | 359-K230JP. Symbiotic Nitrogen-Fixing Soil Bacterium Has an Ability of Methanol Utilization Depending on Rare Earth Elements |
| title_fullStr | 359-K230JP. Symbiotic Nitrogen-Fixing Soil Bacterium Has an Ability of Methanol Utilization Depending on Rare Earth Elements |
| title_full_unstemmed | 359-K230JP. Symbiotic Nitrogen-Fixing Soil Bacterium Has an Ability of Methanol Utilization Depending on Rare Earth Elements |
| title_short | 359-K230JP. Symbiotic Nitrogen-Fixing Soil Bacterium Has an Ability of Methanol Utilization Depending on Rare Earth Elements |
| title_sort | 359 k230jp symbiotic nitrogen fixing soil bacterium has an ability of methanol utilization depending on rare earth elements |
| topic | Agricultural Spatial Analysis and Modelling Agricultural System Analysis and Modelling Sustainable Agricultural Development Farm Management Rural Management Agribusiness Agricultural Management (Others) Animal Production Crop and Pasture Production Other Agricultural and Veterinary Sciences Agricultural Land Management Biological Sciences Food technology Husbandry Livestock Veterinary Sciences |
| url | https://repository.ugm.ac.id/136549/1/The_16th_AAAP%20ALL.pdf |
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