The effect of adding vitamin c and e in native chicken semen extender storaged at temperature 4 °c on semen quality and egg fertility
The objective of this research was to verify the addition of vitamin E (a-tocopherol) and vitamin C (ascorbic acid) as antioxidants and the storage at 4°C on semen quality and fertility. Semen was collected and pooled once time every 3 days from 10 cockerels aged 56 weeks. It was diluted into Buffe...
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| Format: | Conference or Workshop Item |
| Language: | English |
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2010
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| Online Access: | https://repository.ugm.ac.id/137849/1/ISTAP%202010%20%28Ismaya-34%29.pdf |
| _version_ | 1826049568688570368 |
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| author | Asmarawati, Widya Ismaya, Ismaya Yuwanta, Tri |
| author_facet | Asmarawati, Widya Ismaya, Ismaya Yuwanta, Tri |
| author_sort | Asmarawati, Widya |
| collection | UGM |
| description | The objective of this research was to verify the addition of vitamin E (a-tocopherol) and vitamin C (ascorbic acid) as antioxidants and the storage at 4°C on semen quality and fertility. Semen was collected and pooled once time every 3 days from 10 cockerels aged 56 weeks. It was
diluted into Buffer phosphate and devided into seven (7) treatments, i.e ; control; vitamin E (atocopherol 0.1 mM, 0.3 mM, 0.5 mM), and vitamin C (ascorbic acid 2,500 pg/mL; 5,000 pg/mL; 7,500 pg/mL), and then was stored at 4°C for 72 hours. Semen quality was evaluated after 0 hour, 6 hour, 24 hour, 48 hour, and 72 hours storage. Data were analyzed by analyses of variance split subject, and Duncan’s Multiple Range Test (DMRT). A total of 24 hens of Lohman Brown commercial layers were inseminated with 400 x 106 sperm for chicken fertility trials. The result showed that diluents treatment gave significantly effect (P<0.05) on pH and sperm abnormality, but not different on motility and viability. Stored sperm gave significantly effect (P<0.05) on motility, pH, viability, and sperm abnormality. Vitamin E improved the fertilizing ability until 93.10 ± 5,98 % of semen stored for 6 hours at 4°C at the dose of 0,3 mM of semen diluent. It can be concluded that addition of vitamin E to extended chicken semen keeps motility, viability, and sperm normal morphology after 72 hours storage at 4°C. In contrast, vitamin C decrease motility, pH, and sperm viability.
Key words: native chicken, buffer phosphate, vitamin C, vitamin E, semen quality, fertility |
| first_indexed | 2024-03-13T23:48:37Z |
| format | Conference or Workshop Item |
| id | oai:generic.eprints.org:137849 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-13T23:48:37Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1378492016-11-04T07:53:37Z https://repository.ugm.ac.id/137849/ The effect of adding vitamin c and e in native chicken semen extender storaged at temperature 4 °c on semen quality and egg fertility Asmarawati, Widya Ismaya, Ismaya Yuwanta, Tri Animal Reproduction The objective of this research was to verify the addition of vitamin E (a-tocopherol) and vitamin C (ascorbic acid) as antioxidants and the storage at 4°C on semen quality and fertility. Semen was collected and pooled once time every 3 days from 10 cockerels aged 56 weeks. It was diluted into Buffer phosphate and devided into seven (7) treatments, i.e ; control; vitamin E (atocopherol 0.1 mM, 0.3 mM, 0.5 mM), and vitamin C (ascorbic acid 2,500 pg/mL; 5,000 pg/mL; 7,500 pg/mL), and then was stored at 4°C for 72 hours. Semen quality was evaluated after 0 hour, 6 hour, 24 hour, 48 hour, and 72 hours storage. Data were analyzed by analyses of variance split subject, and Duncan’s Multiple Range Test (DMRT). A total of 24 hens of Lohman Brown commercial layers were inseminated with 400 x 106 sperm for chicken fertility trials. The result showed that diluents treatment gave significantly effect (P<0.05) on pH and sperm abnormality, but not different on motility and viability. Stored sperm gave significantly effect (P<0.05) on motility, pH, viability, and sperm abnormality. Vitamin E improved the fertilizing ability until 93.10 ± 5,98 % of semen stored for 6 hours at 4°C at the dose of 0,3 mM of semen diluent. It can be concluded that addition of vitamin E to extended chicken semen keeps motility, viability, and sperm normal morphology after 72 hours storage at 4°C. In contrast, vitamin C decrease motility, pH, and sperm viability. Key words: native chicken, buffer phosphate, vitamin C, vitamin E, semen quality, fertility 2010-10-19 Conference or Workshop Item NonPeerReviewed application/pdf en https://repository.ugm.ac.id/137849/1/ISTAP%202010%20%28Ismaya-34%29.pdf Asmarawati, Widya and Ismaya, Ismaya and Yuwanta, Tri (2010) The effect of adding vitamin c and e in native chicken semen extender storaged at temperature 4 °c on semen quality and egg fertility. In: The 5th ISTAP. International Seminar on Tropical Animal Production: Community Empowerment and Tropical Animal Industry, October 19-22, 2010, Yogyakarta. |
| spellingShingle | Animal Reproduction Asmarawati, Widya Ismaya, Ismaya Yuwanta, Tri The effect of adding vitamin c and e in native chicken semen extender storaged at temperature 4 °c on semen quality and egg fertility |
| title | The effect of adding vitamin c and e in native chicken semen extender storaged at temperature 4 °c on semen quality and egg fertility |
| title_full | The effect of adding vitamin c and e in native chicken semen extender storaged at temperature 4 °c on semen quality and egg fertility |
| title_fullStr | The effect of adding vitamin c and e in native chicken semen extender storaged at temperature 4 °c on semen quality and egg fertility |
| title_full_unstemmed | The effect of adding vitamin c and e in native chicken semen extender storaged at temperature 4 °c on semen quality and egg fertility |
| title_short | The effect of adding vitamin c and e in native chicken semen extender storaged at temperature 4 °c on semen quality and egg fertility |
| title_sort | effect of adding vitamin c and e in native chicken semen extender storaged at temperature 4 °c on semen quality and egg fertility |
| topic | Animal Reproduction |
| url | https://repository.ugm.ac.id/137849/1/ISTAP%202010%20%28Ismaya-34%29.pdf |
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