Computer aided design of efficient phytases
Phytases catalyze the hydrolysis of inorganic phosphate (InsP6) from phytic acid and are able to improve the nutritional quality of phytate rich diet. However, phosphorous in this form (phytate) is largely unavailable to monogastric animals such as poultry and fish, because monogastric animal is lac...
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| Format: | Proceeding Paper |
| Language: | English English English |
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2009
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| Online Access: | http://irep.iium.edu.my/13864/1/KERIE2009_Phytase_poster.pdf http://irep.iium.edu.my/13864/2/KERIE2009_Programme_Book_BTE.pdf http://irep.iium.edu.my/13864/3/LIst_of_WinnersKERIE09.pdf |
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| author | Noorbatcha, Ibrahim Ali Samsudin, Nurhusna Hamzah, Mohd. Salleh Syed Osman Idid, Syed Zahir Idid |
| author_facet | Noorbatcha, Ibrahim Ali Samsudin, Nurhusna Hamzah, Mohd. Salleh Syed Osman Idid, Syed Zahir Idid |
| author_sort | Noorbatcha, Ibrahim Ali |
| collection | IIUM |
| description | Phytases catalyze the hydrolysis of inorganic phosphate (InsP6) from phytic acid and are able to improve the nutritional quality of phytate rich diet. However, phosphorous in this form (phytate) is largely unavailable to monogastric animals such as poultry and fish, because monogastric animal is lacking intestinal phytase. In addition to the unavailability of precious nutrients, phytate not assimilated by animal is excreted in manure and contribute to the phosphorous related environmental pollution problem. Availability of efficient phytases can solve this problem by completely degrading phyates. In this work we have successfully designed a new improved phytase using computed aided protein design (CAPD). We started from a native phytase, identified it’s active site and analyzed the factors that are responsible for phytase binding and hence it’s activity. Armed with this understanding, we have mutated the enzyme and have designed a best mutated enzyme with enhanced binding character. We have identified the active site in Eschericia coli phytase and further validated by docking the known ligands in to the active site using well known Genetic Algorithm (GA) techniques. Transition state of the reaction is determined to calculate the activation energy barrier of the enzyme. Map reaction methods used to determine the transition state and numerical vibrational analysis is carried out to verify transition states. After characterizing the active site, selected amino acid residues are mutated to investigate their effects on the binding strength of this phytase. After a careful manual docking procedure, automated docking is carried out to calculate the strength of the binding before and after mutation. It is found that mutation at position M216R and E219R leads to improvement in the binding strength while mutation at position H17A shows decrease in binding and mutation at A116T have no effect in the binding strength. |
| first_indexed | 2024-03-05T22:47:47Z |
| format | Proceeding Paper |
| id | oai:generic.eprints.org:13864 |
| institution | International Islamic University Malaysia |
| language | English English English |
| last_indexed | 2024-03-05T22:47:47Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:138642021-06-23T02:52:43Z http://irep.iium.edu.my/13864/ Computer aided design of efficient phytases Noorbatcha, Ibrahim Ali Samsudin, Nurhusna Hamzah, Mohd. Salleh Syed Osman Idid, Syed Zahir Idid TP248.13 Biotechnology Phytases catalyze the hydrolysis of inorganic phosphate (InsP6) from phytic acid and are able to improve the nutritional quality of phytate rich diet. However, phosphorous in this form (phytate) is largely unavailable to monogastric animals such as poultry and fish, because monogastric animal is lacking intestinal phytase. In addition to the unavailability of precious nutrients, phytate not assimilated by animal is excreted in manure and contribute to the phosphorous related environmental pollution problem. Availability of efficient phytases can solve this problem by completely degrading phyates. In this work we have successfully designed a new improved phytase using computed aided protein design (CAPD). We started from a native phytase, identified it’s active site and analyzed the factors that are responsible for phytase binding and hence it’s activity. Armed with this understanding, we have mutated the enzyme and have designed a best mutated enzyme with enhanced binding character. We have identified the active site in Eschericia coli phytase and further validated by docking the known ligands in to the active site using well known Genetic Algorithm (GA) techniques. Transition state of the reaction is determined to calculate the activation energy barrier of the enzyme. Map reaction methods used to determine the transition state and numerical vibrational analysis is carried out to verify transition states. After characterizing the active site, selected amino acid residues are mutated to investigate their effects on the binding strength of this phytase. After a careful manual docking procedure, automated docking is carried out to calculate the strength of the binding before and after mutation. It is found that mutation at position M216R and E219R leads to improvement in the binding strength while mutation at position H17A shows decrease in binding and mutation at A116T have no effect in the binding strength. 2009 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/13864/1/KERIE2009_Phytase_poster.pdf application/pdf en http://irep.iium.edu.my/13864/2/KERIE2009_Programme_Book_BTE.pdf application/pdf en http://irep.iium.edu.my/13864/3/LIst_of_WinnersKERIE09.pdf Noorbatcha, Ibrahim Ali and Samsudin, Nurhusna and Hamzah, Mohd. Salleh and Syed Osman Idid, Syed Zahir Idid (2009) Computer aided design of efficient phytases. In: Kulliyyah of Engineering Research and Innovation Exhibition (KERIE 2009), 21-22 January 2009, Gombak. |
| spellingShingle | TP248.13 Biotechnology Noorbatcha, Ibrahim Ali Samsudin, Nurhusna Hamzah, Mohd. Salleh Syed Osman Idid, Syed Zahir Idid Computer aided design of efficient phytases |
| title | Computer aided design of efficient phytases |
| title_full | Computer aided design of efficient phytases |
| title_fullStr | Computer aided design of efficient phytases |
| title_full_unstemmed | Computer aided design of efficient phytases |
| title_short | Computer aided design of efficient phytases |
| title_sort | computer aided design of efficient phytases |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/13864/1/KERIE2009_Phytase_poster.pdf http://irep.iium.edu.my/13864/2/KERIE2009_Programme_Book_BTE.pdf http://irep.iium.edu.my/13864/3/LIst_of_WinnersKERIE09.pdf |
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