Production and Application of Keratinase Enzyme from 4 Strains of Bacillus spp. Isolated from Yogyakarta and Garut City

Processing of waste chicken feathers can be used as a biological treatment with keratinase enzyme. Keratinase enzyme can be produced by microorganisms. Keratinase enzyme expected to be produced from Bacillus spp. which has been previously isolated from Yogyakarta and Garut City. The purpose of th...

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Main Authors: Wandita, Theresia Galuh, Triatmojo, Suharjono, Gumilar, Jajang, Fitriyanto, Nanung Agus
Format: Conference or Workshop Item
Language:English
Published: 2015
Subjects:
Online Access:https://repository.ugm.ac.id/139204/1/Production%20and%20Application%20of%20Keratinase%20Enzyme%20from%204%20Strains%20of%20Bacillus%20spp%20isolated%20from%20Yogyakarta%20and%20Garut%20City.pdf
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author Wandita, Theresia Galuh
Triatmojo, Suharjono
Gumilar, Jajang
Fitriyanto, Nanung Agus
author_facet Wandita, Theresia Galuh
Triatmojo, Suharjono
Gumilar, Jajang
Fitriyanto, Nanung Agus
author_sort Wandita, Theresia Galuh
collection UGM
description Processing of waste chicken feathers can be used as a biological treatment with keratinase enzyme. Keratinase enzyme can be produced by microorganisms. Keratinase enzyme expected to be produced from Bacillus spp. which has been previously isolated from Yogyakarta and Garut City. The purpose of this research was to determine the production of keratinase enzyme produced from Bacillus spp. and apply the keratinase enzyme in the process of degradation of chicken feathers. Research consists to measuring the growth of Bacillus spp. and investigation of degradation of chicken feathers by Bacillus spp. were analyzed descriptively, while data of digested protein by Bacillus spp. analyzed using a split plot design, if there are differences followed by Duncan New Multiple Range Test (DMRT). The results obtained bacterial growth and the ability of degradation was found on Bacillus sp. TD5B. Increasing of growth rate was followed by faster degradation time. Concentration soluble protein by Bacillus meganterium capable of producing higher compared with any others strains. Bacillus meganterium has had highest soluble protein (2,030 mg/ml). The longer degradation time followed by highest concentration soluble protein of feathers. The best incubation time at 8 hours that containing 2,256 mg/ml of soluble protein.
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spelling oai:generic.eprints.org:1392042017-11-22T00:24:20Z https://repository.ugm.ac.id/139204/ Production and Application of Keratinase Enzyme from 4 Strains of Bacillus spp. Isolated from Yogyakarta and Garut City Wandita, Theresia Galuh Triatmojo, Suharjono Gumilar, Jajang Fitriyanto, Nanung Agus Bacteriology Processing of waste chicken feathers can be used as a biological treatment with keratinase enzyme. Keratinase enzyme can be produced by microorganisms. Keratinase enzyme expected to be produced from Bacillus spp. which has been previously isolated from Yogyakarta and Garut City. The purpose of this research was to determine the production of keratinase enzyme produced from Bacillus spp. and apply the keratinase enzyme in the process of degradation of chicken feathers. Research consists to measuring the growth of Bacillus spp. and investigation of degradation of chicken feathers by Bacillus spp. were analyzed descriptively, while data of digested protein by Bacillus spp. analyzed using a split plot design, if there are differences followed by Duncan New Multiple Range Test (DMRT). The results obtained bacterial growth and the ability of degradation was found on Bacillus sp. TD5B. Increasing of growth rate was followed by faster degradation time. Concentration soluble protein by Bacillus meganterium capable of producing higher compared with any others strains. Bacillus meganterium has had highest soluble protein (2,030 mg/ml). The longer degradation time followed by highest concentration soluble protein of feathers. The best incubation time at 8 hours that containing 2,256 mg/ml of soluble protein. 2015 Conference or Workshop Item PeerReviewed application/pdf en https://repository.ugm.ac.id/139204/1/Production%20and%20Application%20of%20Keratinase%20Enzyme%20from%204%20Strains%20of%20Bacillus%20spp%20isolated%20from%20Yogyakarta%20and%20Garut%20City.pdf Wandita, Theresia Galuh and Triatmojo, Suharjono and Gumilar, Jajang and Fitriyanto, Nanung Agus (2015) Production and Application of Keratinase Enzyme from 4 Strains of Bacillus spp. Isolated from Yogyakarta and Garut City. In: The 6th International Seminar on Tropical Animal Production, October 20-22, 2015, Faculty of Animal Science, Universitas Gadjah Mada Yogyakarta, Indonesia.
spellingShingle Bacteriology
Wandita, Theresia Galuh
Triatmojo, Suharjono
Gumilar, Jajang
Fitriyanto, Nanung Agus
Production and Application of Keratinase Enzyme from 4 Strains of Bacillus spp. Isolated from Yogyakarta and Garut City
title Production and Application of Keratinase Enzyme from 4 Strains of Bacillus spp. Isolated from Yogyakarta and Garut City
title_full Production and Application of Keratinase Enzyme from 4 Strains of Bacillus spp. Isolated from Yogyakarta and Garut City
title_fullStr Production and Application of Keratinase Enzyme from 4 Strains of Bacillus spp. Isolated from Yogyakarta and Garut City
title_full_unstemmed Production and Application of Keratinase Enzyme from 4 Strains of Bacillus spp. Isolated from Yogyakarta and Garut City
title_short Production and Application of Keratinase Enzyme from 4 Strains of Bacillus spp. Isolated from Yogyakarta and Garut City
title_sort production and application of keratinase enzyme from 4 strains of bacillus spp isolated from yogyakarta and garut city
topic Bacteriology
url https://repository.ugm.ac.id/139204/1/Production%20and%20Application%20of%20Keratinase%20Enzyme%20from%204%20Strains%20of%20Bacillus%20spp%20isolated%20from%20Yogyakarta%20and%20Garut%20City.pdf
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