Genotypic Profile of Ettawa Grade Goat with Different Head and Neck Color Based on MC1R Gene
The exterior characteristic is the main reason for the most farmers in Indonesia to select and keep Ettawa grade goat. The farmers prefer to keep the goats with black head color instead of brown or mixed colors due to the price of black head color is more expensive. In order to indicate the genetic...
| Main Authors: | , , , , |
|---|---|
| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2015
|
| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/139388/1/C32_%20D%20Maharani%20%282015%29_ISTAP_Rev.pdf |
| _version_ | 1797036631718363136 |
|---|---|
| author | Maharani, Dyah Budisatria, I Gede Suparta Panjono, Panjono Hartatik, Tety Volkandari, Slamet Diah |
| author_facet | Maharani, Dyah Budisatria, I Gede Suparta Panjono, Panjono Hartatik, Tety Volkandari, Slamet Diah |
| author_sort | Maharani, Dyah |
| collection | UGM |
| description | The exterior characteristic is the main reason for the most farmers in Indonesia to select and keep Ettawa grade goat. The farmers prefer to keep the goats with black head color instead of brown or mixed colors due to the price of black head color is more expensive. In order to indicate the genetic molecular basis of different head and neck color of Ettawa grade goats, a comporative analysis of MC1R gene polymorphism was conducted. The Melanocortin – 1 – receptor (MC1R) gene is known as an important candidate gene for the coat color trait. Total thirty Ettawa garde goats were divided in three group : CP ( brown head and neck color with white body color), RP ( brown or black head and neck color with various body color), HP ( black head and neck color with white body color) were used in this study. The blood samples from all groups were collected for DNA isolation. The single nucleotide polymorphisms (SNP 676A G) in exon 1 which located at 676 bp in MC1R gene were obtained by PCR – RFLP Methods for genotyping of the goats byusing earl restriction enzyme. The result showed all of the goats in CP and RP Groups were heterozygote (AG Genotype) which indicated 1.00 for their genotype frequencies. In HP group only had one goat with homozygote animal (GG genotype). Interestingly, none AA genotype found in this study. The A and G allele frequencies were similar 0.5 in both CP and RR group. However, the A allele frequency (0.55) was slightly higher than G allele (0.50)in HP groupe. These results indicated the spread of both alleles were equal in all groups and seems less genetic variability in the goat population study. In conclusion, The SNP 676A G of MC1R gene may be regulated geneticall in Ettawa grade goat with different head color. Further study need to be conducted in detecting the association of the gene that may affect production traits. |
| first_indexed | 2024-03-13T23:49:27Z |
| format | Conference or Workshop Item |
| id | oai:generic.eprints.org:139388 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-13T23:49:27Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1393882017-01-30T03:56:56Z https://repository.ugm.ac.id/139388/ Genotypic Profile of Ettawa Grade Goat with Different Head and Neck Color Based on MC1R Gene Maharani, Dyah Budisatria, I Gede Suparta Panjono, Panjono Hartatik, Tety Volkandari, Slamet Diah Animal Production The exterior characteristic is the main reason for the most farmers in Indonesia to select and keep Ettawa grade goat. The farmers prefer to keep the goats with black head color instead of brown or mixed colors due to the price of black head color is more expensive. In order to indicate the genetic molecular basis of different head and neck color of Ettawa grade goats, a comporative analysis of MC1R gene polymorphism was conducted. The Melanocortin – 1 – receptor (MC1R) gene is known as an important candidate gene for the coat color trait. Total thirty Ettawa garde goats were divided in three group : CP ( brown head and neck color with white body color), RP ( brown or black head and neck color with various body color), HP ( black head and neck color with white body color) were used in this study. The blood samples from all groups were collected for DNA isolation. The single nucleotide polymorphisms (SNP 676A G) in exon 1 which located at 676 bp in MC1R gene were obtained by PCR – RFLP Methods for genotyping of the goats byusing earl restriction enzyme. The result showed all of the goats in CP and RP Groups were heterozygote (AG Genotype) which indicated 1.00 for their genotype frequencies. In HP group only had one goat with homozygote animal (GG genotype). Interestingly, none AA genotype found in this study. The A and G allele frequencies were similar 0.5 in both CP and RR group. However, the A allele frequency (0.55) was slightly higher than G allele (0.50)in HP groupe. These results indicated the spread of both alleles were equal in all groups and seems less genetic variability in the goat population study. In conclusion, The SNP 676A G of MC1R gene may be regulated geneticall in Ettawa grade goat with different head color. Further study need to be conducted in detecting the association of the gene that may affect production traits. 2015-10-22 Conference or Workshop Item PeerReviewed application/pdf en https://repository.ugm.ac.id/139388/1/C32_%20D%20Maharani%20%282015%29_ISTAP_Rev.pdf Maharani, Dyah and Budisatria, I Gede Suparta and Panjono, Panjono and Hartatik, Tety and Volkandari, Slamet Diah (2015) Genotypic Profile of Ettawa Grade Goat with Different Head and Neck Color Based on MC1R Gene. In: The 6th International Seminar on Tropical Animal Production, October 20-22, 2015, Faculty of Animal Science, Universitas Gadjah Mada Yogyakarta, Indonesia. |
| spellingShingle | Animal Production Maharani, Dyah Budisatria, I Gede Suparta Panjono, Panjono Hartatik, Tety Volkandari, Slamet Diah Genotypic Profile of Ettawa Grade Goat with Different Head and Neck Color Based on MC1R Gene |
| title | Genotypic Profile of Ettawa Grade Goat with Different Head and Neck Color Based on MC1R Gene |
| title_full | Genotypic Profile of Ettawa Grade Goat with Different Head and Neck Color Based on MC1R Gene |
| title_fullStr | Genotypic Profile of Ettawa Grade Goat with Different Head and Neck Color Based on MC1R Gene |
| title_full_unstemmed | Genotypic Profile of Ettawa Grade Goat with Different Head and Neck Color Based on MC1R Gene |
| title_short | Genotypic Profile of Ettawa Grade Goat with Different Head and Neck Color Based on MC1R Gene |
| title_sort | genotypic profile of ettawa grade goat with different head and neck color based on mc1r gene |
| topic | Animal Production |
| url | https://repository.ugm.ac.id/139388/1/C32_%20D%20Maharani%20%282015%29_ISTAP_Rev.pdf |
| work_keys_str_mv | AT maharanidyah genotypicprofileofettawagradegoatwithdifferentheadandneckcolorbasedonmc1rgene AT budisatriaigedesuparta genotypicprofileofettawagradegoatwithdifferentheadandneckcolorbasedonmc1rgene AT panjonopanjono genotypicprofileofettawagradegoatwithdifferentheadandneckcolorbasedonmc1rgene AT hartatiktety genotypicprofileofettawagradegoatwithdifferentheadandneckcolorbasedonmc1rgene AT volkandarislametdiah genotypicprofileofettawagradegoatwithdifferentheadandneckcolorbasedonmc1rgene |