Chinese hamster ovary (CHO-K1) cells expressed native insulin-like growth factor-1 (IGF-1) gene towards efficient mammalian cell culture host system
Insulin-like growth factor-1 (IGF-1) has been shown to promote cell proliferation and inhibit apoptosis of cells. These are two characteristics of mammalian cell culture which may lead to high density cell culture producing optimal desired yield of bioproducts. An inherent secretion of IGF-1 protei...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Academic Journals
2011
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| Online Access: | http://irep.iium.edu.my/14207/1/Mohamed_et_al_1.pdf |
| _version_ | 1796876140685557760 |
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| author | Packeer Mohamed, Vasila Hashim, Yumi Zuhanis Has-Yun Amid, Azura Mel, Maizirwan Kamarulzaman, Abdul Razak Abdul Wahab, Muhammad Adil Mohmad Saberi, Salfarina Ezrina |
| author_facet | Packeer Mohamed, Vasila Hashim, Yumi Zuhanis Has-Yun Amid, Azura Mel, Maizirwan Kamarulzaman, Abdul Razak Abdul Wahab, Muhammad Adil Mohmad Saberi, Salfarina Ezrina |
| author_sort | Packeer Mohamed, Vasila |
| collection | IIUM |
| description | Insulin-like growth factor-1 (IGF-1) has been shown to promote cell proliferation and inhibit apoptosis of
cells. These are two characteristics of mammalian cell culture which may lead to high density cell culture producing optimal desired yield of bioproducts. An inherent secretion of IGF-1 protein from host cells into the culture media is hypothesized to enable reduction or removable of serum from culture media, thus reducing cost. This study was set to investigate the IGF-1 gene expression in Chinese
hamster ovary (CHO-K1) cells. The cells were first cultured in T-flask with three independent experiments. An 8-hourly sampling for responses (glucose, lactate, total protein and biomass) was done. PCR-based method was performed to study the expression of IGF-1 gene. To this end, it was confirmed that CHO-K1 cells used in this study expressed IGF-1 gene. The study also provides the baseline data on kinetics and biochemical responses of CHO-K1 cell growth. Together, the data would be particularly useful for further studies using CHO-K1 cells as efficient mammalian cell culture host system to produce biologics. |
| first_indexed | 2024-03-05T22:48:15Z |
| format | Article |
| id | oai:generic.eprints.org:14207 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T22:48:15Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | oai:generic.eprints.org:142072013-07-02T01:20:01Z http://irep.iium.edu.my/14207/ Chinese hamster ovary (CHO-K1) cells expressed native insulin-like growth factor-1 (IGF-1) gene towards efficient mammalian cell culture host system Packeer Mohamed, Vasila Hashim, Yumi Zuhanis Has-Yun Amid, Azura Mel, Maizirwan Kamarulzaman, Abdul Razak Abdul Wahab, Muhammad Adil Mohmad Saberi, Salfarina Ezrina TP248.13 Biotechnology Insulin-like growth factor-1 (IGF-1) has been shown to promote cell proliferation and inhibit apoptosis of cells. These are two characteristics of mammalian cell culture which may lead to high density cell culture producing optimal desired yield of bioproducts. An inherent secretion of IGF-1 protein from host cells into the culture media is hypothesized to enable reduction or removable of serum from culture media, thus reducing cost. This study was set to investigate the IGF-1 gene expression in Chinese hamster ovary (CHO-K1) cells. The cells were first cultured in T-flask with three independent experiments. An 8-hourly sampling for responses (glucose, lactate, total protein and biomass) was done. PCR-based method was performed to study the expression of IGF-1 gene. To this end, it was confirmed that CHO-K1 cells used in this study expressed IGF-1 gene. The study also provides the baseline data on kinetics and biochemical responses of CHO-K1 cell growth. Together, the data would be particularly useful for further studies using CHO-K1 cells as efficient mammalian cell culture host system to produce biologics. Academic Journals 2011-12-16 Article PeerReviewed application/pdf en http://irep.iium.edu.my/14207/1/Mohamed_et_al_1.pdf Packeer Mohamed, Vasila and Hashim, Yumi Zuhanis Has-Yun and Amid, Azura and Mel, Maizirwan and Kamarulzaman, Abdul Razak and Abdul Wahab, Muhammad Adil and Mohmad Saberi, Salfarina Ezrina (2011) Chinese hamster ovary (CHO-K1) cells expressed native insulin-like growth factor-1 (IGF-1) gene towards efficient mammalian cell culture host system. African Journal of Biotechnology, 10 (81). pp. 18716-18721. ISSN 1684–5315 http://www.academicjournals.org/AJB/PDF/pdf2011/16DecConf/Mohamed%20et%20al%201.pdf 10.5897/AJB11.274 |
| spellingShingle | TP248.13 Biotechnology Packeer Mohamed, Vasila Hashim, Yumi Zuhanis Has-Yun Amid, Azura Mel, Maizirwan Kamarulzaman, Abdul Razak Abdul Wahab, Muhammad Adil Mohmad Saberi, Salfarina Ezrina Chinese hamster ovary (CHO-K1) cells expressed native insulin-like growth factor-1 (IGF-1) gene towards efficient mammalian cell culture host system |
| title | Chinese hamster ovary (CHO-K1) cells expressed native insulin-like growth factor-1 (IGF-1) gene towards
efficient mammalian cell culture host system
|
| title_full | Chinese hamster ovary (CHO-K1) cells expressed native insulin-like growth factor-1 (IGF-1) gene towards
efficient mammalian cell culture host system
|
| title_fullStr | Chinese hamster ovary (CHO-K1) cells expressed native insulin-like growth factor-1 (IGF-1) gene towards
efficient mammalian cell culture host system
|
| title_full_unstemmed | Chinese hamster ovary (CHO-K1) cells expressed native insulin-like growth factor-1 (IGF-1) gene towards
efficient mammalian cell culture host system
|
| title_short | Chinese hamster ovary (CHO-K1) cells expressed native insulin-like growth factor-1 (IGF-1) gene towards
efficient mammalian cell culture host system
|
| title_sort | chinese hamster ovary cho k1 cells expressed native insulin like growth factor 1 igf 1 gene towards efficient mammalian cell culture host system |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/14207/1/Mohamed_et_al_1.pdf |
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