Development of culture inoculum for scale-up production of citric acid from oil palm empty fruit bunches by Aspergillus niger

An extensive study on fungal inoculum was carried out to develop an effective culture (broth) inoculum for citric acid production from oil palm empty fruit bunches through solid state bioconversion. The effect of spores age, storage time of spore suspension inoculum, doses of spore suspension inocul...

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Main Authors: Alam, Md. Zahangir, Bari, Md. Niamul, Muyibi, Suleyman Aremu, Jamal, Parveen, Al-Mamun, Abdullah
Format: Proceeding Paper
Language:English
English
Published: 2011
Subjects:
Online Access:http://irep.iium.edu.my/14392/1/Full-paper-revised-M10019.pdf
http://irep.iium.edu.my/14392/4/science_14392.pdf
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author Alam, Md. Zahangir
Bari, Md. Niamul
Muyibi, Suleyman Aremu
Jamal, Parveen
Al-Mamun, Abdullah
author_facet Alam, Md. Zahangir
Bari, Md. Niamul
Muyibi, Suleyman Aremu
Jamal, Parveen
Al-Mamun, Abdullah
author_sort Alam, Md. Zahangir
collection IIUM
description An extensive study on fungal inoculum was carried out to develop an effective culture (broth) inoculum for citric acid production from oil palm empty fruit bunches through solid state bioconversion. The effect of spores age, storage time of spore suspension inoculum, doses of spore suspension inoculum to prepare culture inoculum, ages of culture inoculum and doses of culture inoculum (concentration of biomass) on citric acid production were evaluated. It was found that the optimum age of spore was 3-4 days for the preparation of spore suspension inoculum and the maximum storage time of spore suspension inoculum was 2 days. Furthermore, the optimum dose of spore suspension inoculum was 20% (v/v) to prepare culture (broth) inoculum. The culture inoculum of 48 hrs age with biomass concentration of 0.82±0.3 g/L showed the highest production of citric acid compared to other percentage of spore suspension inoculum and culture age for all doses of culture inoculum. The highest production of citric acid of 367.4±2 g/kg-EFB was obtained with 25% (v/w) (0.21 g biomass/kg substrate) culture inoculum after 7 days of bioconversion.
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spelling oai:generic.eprints.org:143922013-08-20T06:20:13Z http://irep.iium.edu.my/14392/ Development of culture inoculum for scale-up production of citric acid from oil palm empty fruit bunches by Aspergillus niger Alam, Md. Zahangir Bari, Md. Niamul Muyibi, Suleyman Aremu Jamal, Parveen Al-Mamun, Abdullah TP248.13 Biotechnology An extensive study on fungal inoculum was carried out to develop an effective culture (broth) inoculum for citric acid production from oil palm empty fruit bunches through solid state bioconversion. The effect of spores age, storage time of spore suspension inoculum, doses of spore suspension inoculum to prepare culture inoculum, ages of culture inoculum and doses of culture inoculum (concentration of biomass) on citric acid production were evaluated. It was found that the optimum age of spore was 3-4 days for the preparation of spore suspension inoculum and the maximum storage time of spore suspension inoculum was 2 days. Furthermore, the optimum dose of spore suspension inoculum was 20% (v/v) to prepare culture (broth) inoculum. The culture inoculum of 48 hrs age with biomass concentration of 0.82±0.3 g/L showed the highest production of citric acid compared to other percentage of spore suspension inoculum and culture age for all doses of culture inoculum. The highest production of citric acid of 367.4±2 g/kg-EFB was obtained with 25% (v/w) (0.21 g biomass/kg substrate) culture inoculum after 7 days of bioconversion. 2011-11 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/14392/1/Full-paper-revised-M10019.pdf application/pdf en http://irep.iium.edu.my/14392/4/science_14392.pdf Alam, Md. Zahangir and Bari, Md. Niamul and Muyibi, Suleyman Aremu and Jamal, Parveen and Al-Mamun, Abdullah (2011) Development of culture inoculum for scale-up production of citric acid from oil palm empty fruit bunches by Aspergillus niger. In: International Conference on Environment Science and Biotechnology (ICESB 2011), 25-27 November 2011, Maldives. http://www.sciencedirect.com/science/article/pii/S1878029611006980
spellingShingle TP248.13 Biotechnology
Alam, Md. Zahangir
Bari, Md. Niamul
Muyibi, Suleyman Aremu
Jamal, Parveen
Al-Mamun, Abdullah
Development of culture inoculum for scale-up production of citric acid from oil palm empty fruit bunches by Aspergillus niger
title Development of culture inoculum for scale-up production of citric acid from oil palm empty fruit bunches by Aspergillus niger
title_full Development of culture inoculum for scale-up production of citric acid from oil palm empty fruit bunches by Aspergillus niger
title_fullStr Development of culture inoculum for scale-up production of citric acid from oil palm empty fruit bunches by Aspergillus niger
title_full_unstemmed Development of culture inoculum for scale-up production of citric acid from oil palm empty fruit bunches by Aspergillus niger
title_short Development of culture inoculum for scale-up production of citric acid from oil palm empty fruit bunches by Aspergillus niger
title_sort development of culture inoculum for scale up production of citric acid from oil palm empty fruit bunches by aspergillus niger
topic TP248.13 Biotechnology
url http://irep.iium.edu.my/14392/1/Full-paper-revised-M10019.pdf
http://irep.iium.edu.my/14392/4/science_14392.pdf
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