Purification and properties of a new dehalogenase enzyme from Pseudomonas sp. B6P grow in 3- chloropropionate (3CP)
Halogenated compounds are widely used in agriculture and industries and have been associated with environmental pollution. Degradation of 3-chloropropionate (3CP) by microorganism has been established and this enzyme could only remove halogen atom at the �- position of 3-carbon alkanoic acids. Pseud...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Academic Journals
2011
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| Online Access: | http://irep.iium.edu.my/1606/1/PaperFahrul_TgAbdul_Hamid.pdf |
| _version_ | 1796874799799074816 |
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| author | Tengku Abdul Hamid, Tengku Haziyamin Hamid, A. A. A Zulkifly, Nurul Asma Hasliza Hamdan, Sallehuddin Ariffin, Shahrul Hisham Zainal Huyop, Fahrul Zaman |
| author_facet | Tengku Abdul Hamid, Tengku Haziyamin Hamid, A. A. A Zulkifly, Nurul Asma Hasliza Hamdan, Sallehuddin Ariffin, Shahrul Hisham Zainal Huyop, Fahrul Zaman |
| author_sort | Tengku Abdul Hamid, Tengku Haziyamin |
| collection | IIUM |
| description | Halogenated compounds are widely used in agriculture and industries and have been associated with environmental pollution. Degradation of 3-chloropropionate (3CP) by microorganism has been established and this enzyme could only remove halogen atom at the �- position of 3-carbon alkanoic acids. Pseudomonas sp. B6P was originally isolated from paddy field which was able to degrade 3CP therefore, suggesting it may have some desirable properties. The enzyme was purified from cell-free extracts having a monomer of 56,000 Da. It was found to be stable between pH 5 to 8 and its optimal pH was 8. Its activity was not affected by metal ions such as Mn2+, Fe3+ and Mg2+, but was inhibited by Hg2+ and Ag2+. The enzyme is specific for 3CP, and the Km value (0.20 mM ± 0.05). |
| first_indexed | 2024-03-05T22:29:37Z |
| format | Article |
| id | oai:generic.eprints.org:1606 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T22:29:37Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | oai:generic.eprints.org:16062011-09-05T06:43:10Z http://irep.iium.edu.my/1606/ Purification and properties of a new dehalogenase enzyme from Pseudomonas sp. B6P grow in 3- chloropropionate (3CP) Tengku Abdul Hamid, Tengku Haziyamin Hamid, A. A. A Zulkifly, Nurul Asma Hasliza Hamdan, Sallehuddin Ariffin, Shahrul Hisham Zainal Huyop, Fahrul Zaman Q Science (General) Halogenated compounds are widely used in agriculture and industries and have been associated with environmental pollution. Degradation of 3-chloropropionate (3CP) by microorganism has been established and this enzyme could only remove halogen atom at the �- position of 3-carbon alkanoic acids. Pseudomonas sp. B6P was originally isolated from paddy field which was able to degrade 3CP therefore, suggesting it may have some desirable properties. The enzyme was purified from cell-free extracts having a monomer of 56,000 Da. It was found to be stable between pH 5 to 8 and its optimal pH was 8. Its activity was not affected by metal ions such as Mn2+, Fe3+ and Mg2+, but was inhibited by Hg2+ and Ag2+. The enzyme is specific for 3CP, and the Km value (0.20 mM ± 0.05). Academic Journals 2011-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/1606/1/PaperFahrul_TgAbdul_Hamid.pdf Tengku Abdul Hamid, Tengku Haziyamin and Hamid, A. A. A and Zulkifly, Nurul Asma Hasliza and Hamdan, Sallehuddin and Ariffin, Shahrul Hisham Zainal and Huyop, Fahrul Zaman (2011) Purification and properties of a new dehalogenase enzyme from Pseudomonas sp. B6P grow in 3- chloropropionate (3CP). African Journal of Biotechnology, 10 (4). pp. 610-614. ISSN ISSN 1684–5315 http://www.academicjournals.org/AJB |
| spellingShingle | Q Science (General) Tengku Abdul Hamid, Tengku Haziyamin Hamid, A. A. A Zulkifly, Nurul Asma Hasliza Hamdan, Sallehuddin Ariffin, Shahrul Hisham Zainal Huyop, Fahrul Zaman Purification and properties of a new dehalogenase enzyme from Pseudomonas sp. B6P grow in 3- chloropropionate (3CP) |
| title | Purification and properties of a new dehalogenase enzyme from Pseudomonas sp. B6P grow in 3- chloropropionate (3CP) |
| title_full | Purification and properties of a new dehalogenase enzyme from Pseudomonas sp. B6P grow in 3- chloropropionate (3CP) |
| title_fullStr | Purification and properties of a new dehalogenase enzyme from Pseudomonas sp. B6P grow in 3- chloropropionate (3CP) |
| title_full_unstemmed | Purification and properties of a new dehalogenase enzyme from Pseudomonas sp. B6P grow in 3- chloropropionate (3CP) |
| title_short | Purification and properties of a new dehalogenase enzyme from Pseudomonas sp. B6P grow in 3- chloropropionate (3CP) |
| title_sort | purification and properties of a new dehalogenase enzyme from pseudomonas sp b6p grow in 3 chloropropionate 3cp |
| topic | Q Science (General) |
| url | http://irep.iium.edu.my/1606/1/PaperFahrul_TgAbdul_Hamid.pdf |
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