Isolation of fuxoxanthin and fatty analysis from Padina australis and cytotoxicity effect of fucoxanthin of human lung cancer (H1299) cell lines
Fucoxanthin has been successfully isolated from species of Malaysian brown seaweed, namely P. australis. The purity of the fucoxanthin is >98% as indicated by High Performance Liquid Chromatography analysis. This seaweed also contained a considerable amount of unsaturated fatty acids. There are t...
| Main Authors: | , , , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2011
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| Online Access: | http://irep.iium.edu.my/16360/1/Isolation_of_Fucoxanthin_and_fatty_acids_analysis.pdf |
| _version_ | 1796876308297285632 |
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| author | Noviendri, Dedi Jaswir, Irwandi Mohd. Salleh, Hamzah Taher, Muhammad Miyashita, Kazuo |
| author_facet | Noviendri, Dedi Jaswir, Irwandi Mohd. Salleh, Hamzah Taher, Muhammad Miyashita, Kazuo |
| author_sort | Noviendri, Dedi |
| collection | IIUM |
| description | Fucoxanthin has been successfully isolated from species of Malaysian brown seaweed, namely P. australis. The purity of the fucoxanthin is >98% as indicated by High Performance Liquid Chromatography analysis. This seaweed also contained a considerable amount of unsaturated fatty acids. There are thirteen fatty acids were detected with Gas Chromatography. However, in terms of fatty acid methyl ester (FAMEs) of eicosapentanoic acid (C20:5n-3), arachidonic acid (C20:4n-6), linoleic acid (C18:2n-6) and alpha-linolenic acid (C18:3n-3) contents from P.australis was found (2.06, 9.50, 6.37, and 2.83 %, respectively). For saturated fatty acids, palmitic acid (C16:0) was found to be the major fatty acid about 23.97%. Furthermore, data obtained from the MTT assay indicated that fucoxanthin reduced the viability of H1299 cell lines, showing an IC50 value was 2.45 mM. |
| first_indexed | 2024-03-05T22:50:35Z |
| format | Proceeding Paper |
| id | oai:generic.eprints.org:16360 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T22:50:35Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:163602021-06-03T04:16:46Z http://irep.iium.edu.my/16360/ Isolation of fuxoxanthin and fatty analysis from Padina australis and cytotoxicity effect of fucoxanthin of human lung cancer (H1299) cell lines Noviendri, Dedi Jaswir, Irwandi Mohd. Salleh, Hamzah Taher, Muhammad Miyashita, Kazuo TP Chemical technology Fucoxanthin has been successfully isolated from species of Malaysian brown seaweed, namely P. australis. The purity of the fucoxanthin is >98% as indicated by High Performance Liquid Chromatography analysis. This seaweed also contained a considerable amount of unsaturated fatty acids. There are thirteen fatty acids were detected with Gas Chromatography. However, in terms of fatty acid methyl ester (FAMEs) of eicosapentanoic acid (C20:5n-3), arachidonic acid (C20:4n-6), linoleic acid (C18:2n-6) and alpha-linolenic acid (C18:3n-3) contents from P.australis was found (2.06, 9.50, 6.37, and 2.83 %, respectively). For saturated fatty acids, palmitic acid (C16:0) was found to be the major fatty acid about 23.97%. Furthermore, data obtained from the MTT assay indicated that fucoxanthin reduced the viability of H1299 cell lines, showing an IC50 value was 2.45 mM. 2011 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/16360/1/Isolation_of_Fucoxanthin_and_fatty_acids_analysis.pdf Noviendri, Dedi and Jaswir, Irwandi and Mohd. Salleh, Hamzah and Taher, Muhammad and Miyashita, Kazuo (2011) Isolation of fuxoxanthin and fatty analysis from Padina australis and cytotoxicity effect of fucoxanthin of human lung cancer (H1299) cell lines. In: Proceedings of the 2nd International Conference on Biotechnology Engineering ICBioE'11, 17-19 May 2011, Kuala Lumpur. http://www.iium.edu.my/icbioe/2011/ |
| spellingShingle | TP Chemical technology Noviendri, Dedi Jaswir, Irwandi Mohd. Salleh, Hamzah Taher, Muhammad Miyashita, Kazuo Isolation of fuxoxanthin and fatty analysis from Padina australis and cytotoxicity effect of fucoxanthin of human lung cancer (H1299) cell lines |
| title | Isolation of fuxoxanthin and fatty analysis from Padina australis and cytotoxicity effect of fucoxanthin of human lung cancer (H1299) cell lines |
| title_full | Isolation of fuxoxanthin and fatty analysis from Padina australis and cytotoxicity effect of fucoxanthin of human lung cancer (H1299) cell lines |
| title_fullStr | Isolation of fuxoxanthin and fatty analysis from Padina australis and cytotoxicity effect of fucoxanthin of human lung cancer (H1299) cell lines |
| title_full_unstemmed | Isolation of fuxoxanthin and fatty analysis from Padina australis and cytotoxicity effect of fucoxanthin of human lung cancer (H1299) cell lines |
| title_short | Isolation of fuxoxanthin and fatty analysis from Padina australis and cytotoxicity effect of fucoxanthin of human lung cancer (H1299) cell lines |
| title_sort | isolation of fuxoxanthin and fatty analysis from padina australis and cytotoxicity effect of fucoxanthin of human lung cancer h1299 cell lines |
| topic | TP Chemical technology |
| url | http://irep.iium.edu.my/16360/1/Isolation_of_Fucoxanthin_and_fatty_acids_analysis.pdf |
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