| Summary: | ABSTRAK
Malaria is a major health problem in the Province of North Sulawesi, espesially in Tombatu subdistrict. The development of chloroquine resistance strain has been reported in 2003. Mutation in pfmdr-1 gene especially in codon 86 has been corelated with chloroquine resistance. However, in North Sulawesi the corelation between pjrndr-1 gene mutation and the resistance to chloroquine hasn't been identified. Single Stranded Conformation Polymorphism (SSCP) analysis is a simple methode to detect mutation in certain gene. The present study is aimed to find the resistance parasites from Tombatu subdistrict, to know whether pfmdr-1 gene isolate from Tombatu can be amplificated with primer from Thailand isolate, and whether there is any mutation on pfmdr-1 gene fragment of resistance parasites by PCR-SSCP technique.
The resistance parasites were identified using WHO 2001 in vivo chloroquine resistance test. The pfmdr-1 gene were isolated from blood samples and amplified using published primers. The mutation on the pfmdr-1 gene was identified by SSCP analysis of the PCR product on polyacrilamid gel electrophoresis.
The in vivo resistance test showed that among 15 subject to be studied,
pfmdr-1 gene isola
amplificated using primer from Thailand isolate, PCR-SSCP analysis on PCR amplification product of pfmdr-1 gene fragment indicated that there isn't any mutation of the gene fragment of resistant parasites.
Keywords: Chloroquine resistance, pfmdr-1 gene mutation, PCR-SSCP Analysis
.
|
|---|