Analisis PCR-SSCP Fragmen Gen pfmdr-1 Plasmodium isolat Kecamatan Tombatu, Kabupaten Minahasa Selatan, Propinsi Sulawesi Utara yang Resisten Terhadap Klorokuin = PCR-SSCP Analysis of pfmdr-1 Gene of ...
ABSTRAK Malaria is a major health problem in the Province of North Sulawesi, espesially in Tombatu subdistrict. The development of chloroquine resistance strain has been reported in 2003. Mutation in pfmdr-1 gene especially in codon 86 has been corelated with chloroquine resistance. However, in Nort...
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[Yogyakarta] : Universitas Gadjah Mada
2006
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author | Perpustakaan UGM, i-lib |
author_facet | Perpustakaan UGM, i-lib |
author_sort | Perpustakaan UGM, i-lib |
collection | UGM |
description | ABSTRAK
Malaria is a major health problem in the Province of North Sulawesi, espesially in Tombatu subdistrict. The development of chloroquine resistance strain has been reported in 2003. Mutation in pfmdr-1 gene especially in codon 86 has been corelated with chloroquine resistance. However, in North Sulawesi the corelation between pjrndr-1 gene mutation and the resistance to chloroquine hasn't been identified. Single Stranded Conformation Polymorphism (SSCP) analysis is a simple methode to detect mutation in certain gene. The present study is aimed to find the resistance parasites from Tombatu subdistrict, to know whether pfmdr-1 gene isolate from Tombatu can be amplificated with primer from Thailand isolate, and whether there is any mutation on pfmdr-1 gene fragment of resistance parasites by PCR-SSCP technique.
The resistance parasites were identified using WHO 2001 in vivo chloroquine resistance test. The pfmdr-1 gene were isolated from blood samples and amplified using published primers. The mutation on the pfmdr-1 gene was identified by SSCP analysis of the PCR product on polyacrilamid gel electrophoresis.
The in vivo resistance test showed that among 15 subject to be studied,
pfmdr-1 gene isola
amplificated using primer from Thailand isolate, PCR-SSCP analysis on PCR amplification product of pfmdr-1 gene fragment indicated that there isn't any mutation of the gene fragment of resistant parasites.
Keywords: Chloroquine resistance, pfmdr-1 gene mutation, PCR-SSCP Analysis
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format | Article |
id | oai:generic.eprints.org:17278 |
institution | Universiti Gadjah Mada |
last_indexed | 2024-03-13T18:32:21Z |
publishDate | 2006 |
publisher | [Yogyakarta] : Universitas Gadjah Mada |
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spelling | oai:generic.eprints.org:172782014-06-18T00:25:30Z https://repository.ugm.ac.id/17278/ Analisis PCR-SSCP Fragmen Gen pfmdr-1 Plasmodium isolat Kecamatan Tombatu, Kabupaten Minahasa Selatan, Propinsi Sulawesi Utara yang Resisten Terhadap Klorokuin = PCR-SSCP Analysis of pfmdr-1 Gene of ... Perpustakaan UGM, i-lib Jurnal i-lib UGM ABSTRAK Malaria is a major health problem in the Province of North Sulawesi, espesially in Tombatu subdistrict. The development of chloroquine resistance strain has been reported in 2003. Mutation in pfmdr-1 gene especially in codon 86 has been corelated with chloroquine resistance. However, in North Sulawesi the corelation between pjrndr-1 gene mutation and the resistance to chloroquine hasn't been identified. Single Stranded Conformation Polymorphism (SSCP) analysis is a simple methode to detect mutation in certain gene. The present study is aimed to find the resistance parasites from Tombatu subdistrict, to know whether pfmdr-1 gene isolate from Tombatu can be amplificated with primer from Thailand isolate, and whether there is any mutation on pfmdr-1 gene fragment of resistance parasites by PCR-SSCP technique. The resistance parasites were identified using WHO 2001 in vivo chloroquine resistance test. The pfmdr-1 gene were isolated from blood samples and amplified using published primers. The mutation on the pfmdr-1 gene was identified by SSCP analysis of the PCR product on polyacrilamid gel electrophoresis. The in vivo resistance test showed that among 15 subject to be studied, pfmdr-1 gene isola amplificated using primer from Thailand isolate, PCR-SSCP analysis on PCR amplification product of pfmdr-1 gene fragment indicated that there isn't any mutation of the gene fragment of resistant parasites. Keywords: Chloroquine resistance, pfmdr-1 gene mutation, PCR-SSCP Analysis . [Yogyakarta] : Universitas Gadjah Mada 2006 Article NonPeerReviewed Perpustakaan UGM, i-lib (2006) Analisis PCR-SSCP Fragmen Gen pfmdr-1 Plasmodium isolat Kecamatan Tombatu, Kabupaten Minahasa Selatan, Propinsi Sulawesi Utara yang Resisten Terhadap Klorokuin = PCR-SSCP Analysis of pfmdr-1 Gene of ... Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=29 |
spellingShingle | Jurnal i-lib UGM Perpustakaan UGM, i-lib Analisis PCR-SSCP Fragmen Gen pfmdr-1 Plasmodium isolat Kecamatan Tombatu, Kabupaten Minahasa Selatan, Propinsi Sulawesi Utara yang Resisten Terhadap Klorokuin = PCR-SSCP Analysis of pfmdr-1 Gene of ... |
title | Analisis PCR-SSCP Fragmen Gen pfmdr-1 Plasmodium isolat Kecamatan Tombatu, Kabupaten Minahasa Selatan, Propinsi Sulawesi Utara yang Resisten Terhadap Klorokuin = PCR-SSCP Analysis of pfmdr-1 Gene of ... |
title_full | Analisis PCR-SSCP Fragmen Gen pfmdr-1 Plasmodium isolat Kecamatan Tombatu, Kabupaten Minahasa Selatan, Propinsi Sulawesi Utara yang Resisten Terhadap Klorokuin = PCR-SSCP Analysis of pfmdr-1 Gene of ... |
title_fullStr | Analisis PCR-SSCP Fragmen Gen pfmdr-1 Plasmodium isolat Kecamatan Tombatu, Kabupaten Minahasa Selatan, Propinsi Sulawesi Utara yang Resisten Terhadap Klorokuin = PCR-SSCP Analysis of pfmdr-1 Gene of ... |
title_full_unstemmed | Analisis PCR-SSCP Fragmen Gen pfmdr-1 Plasmodium isolat Kecamatan Tombatu, Kabupaten Minahasa Selatan, Propinsi Sulawesi Utara yang Resisten Terhadap Klorokuin = PCR-SSCP Analysis of pfmdr-1 Gene of ... |
title_short | Analisis PCR-SSCP Fragmen Gen pfmdr-1 Plasmodium isolat Kecamatan Tombatu, Kabupaten Minahasa Selatan, Propinsi Sulawesi Utara yang Resisten Terhadap Klorokuin = PCR-SSCP Analysis of pfmdr-1 Gene of ... |
title_sort | analisis pcr sscp fragmen gen pfmdr 1 plasmodium isolat kecamatan tombatu kabupaten minahasa selatan propinsi sulawesi utara yang resisten terhadap klorokuin pcr sscp analysis of pfmdr 1 gene of |
topic | Jurnal i-lib UGM |
work_keys_str_mv | AT perpustakaanugmilib analisispcrsscpfragmengenpfmdr1plasmodiumisolatkecamatantombatukabupatenminahasaselatanpropinsisulawesiutarayangresistenterhadapklorokuinpcrsscpanalysisofpfmdr1geneof |