Skrining senyawa bioaktif dari beberapa ekstrak tumbuhan asal kawasan hutan Kalimantan Tengah dan isolasi senyawa bioaktif Fibraurea chloroleuca Miers = Bioactive compound screening from ...
ABSTRAK Indonesia is known for its largest biodiversity on the world. However, up to now they have not been studied completely for their utilization and development especially as drug resources. Bioactive screening on 14 extracts (chloroform and methanol extracts of medicinal plants) using Brine Shr...
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| Format: | Article |
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[Yogyakarta] : Universitas Gadjah Mada
2006
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| _version_ | 1826031054169833472 |
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| author | Perpustakaan UGM, i-lib |
| author_facet | Perpustakaan UGM, i-lib |
| author_sort | Perpustakaan UGM, i-lib |
| collection | UGM |
| description | ABSTRAK
Indonesia is known for its largest biodiversity on the world. However, up to now they have not been studied completely for their utilization and development especially as drug resources. Bioactive screening on 14 extracts (chloroform and methanol extracts of medicinal plants) using Brine Shrimp Lethality (BST, using larvae Artemia salina) assay (500 and 1000 (iy/ml) gave 17 extracts potential (100% larvae death) to be developed as bioactive compounds resources. Further screening of these 17 extracts at a lower dose (100 (iy/ml), 10 extracts (100% larvae death) were obtained. One of those extracts is chloroform extracts of # 03 bfar 029 identified as Fibraurea chloroleuca Miers.
Bioassay (BST) guided isolation of F. chloroleuca extract, the most toxic isolate (bl) was obtained (LC^ 4.5 ny/ml). The isolate (bl) at the highest tested dose (500 ny/ml) inhibited 20,08% of the growth of HeLa cell in vitro.
Isolate (bl) appears as orange powder, having melting point at 182.3-183.0°C and X^ (CHCL3) at241 and 345 nm. Based onTLC pictured visualized by Dragendorff, bl is confirmed as an alkaloid. Mass spectrum (EIMS) data of bl showed the highest mass peak at m/z 351 (75%). The odd mass number strengthens the conclusions that bl is an alkaloid having odd number of -N- atom. The TR spectrum (KBr) indicates the present -OH, =CH, -CH, and C=C-C=O, secondary amine functional groups, in addition aromatic ring absorption band is also observed.
Keywords: Fibraurea chloroleuca Miers., bioactive, BST, HeLa cell |
| first_indexed | 2024-03-05T22:51:46Z |
| format | Article |
| id | oai:generic.eprints.org:17286 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T18:32:22Z |
| publishDate | 2006 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:172862014-06-18T00:26:14Z https://repository.ugm.ac.id/17286/ Skrining senyawa bioaktif dari beberapa ekstrak tumbuhan asal kawasan hutan Kalimantan Tengah dan isolasi senyawa bioaktif Fibraurea chloroleuca Miers = Bioactive compound screening from ... Perpustakaan UGM, i-lib Jurnal i-lib UGM ABSTRAK Indonesia is known for its largest biodiversity on the world. However, up to now they have not been studied completely for their utilization and development especially as drug resources. Bioactive screening on 14 extracts (chloroform and methanol extracts of medicinal plants) using Brine Shrimp Lethality (BST, using larvae Artemia salina) assay (500 and 1000 (iy/ml) gave 17 extracts potential (100% larvae death) to be developed as bioactive compounds resources. Further screening of these 17 extracts at a lower dose (100 (iy/ml), 10 extracts (100% larvae death) were obtained. One of those extracts is chloroform extracts of # 03 bfar 029 identified as Fibraurea chloroleuca Miers. Bioassay (BST) guided isolation of F. chloroleuca extract, the most toxic isolate (bl) was obtained (LC^ 4.5 ny/ml). The isolate (bl) at the highest tested dose (500 ny/ml) inhibited 20,08% of the growth of HeLa cell in vitro. Isolate (bl) appears as orange powder, having melting point at 182.3-183.0°C and X^ (CHCL3) at241 and 345 nm. Based onTLC pictured visualized by Dragendorff, bl is confirmed as an alkaloid. Mass spectrum (EIMS) data of bl showed the highest mass peak at m/z 351 (75%). The odd mass number strengthens the conclusions that bl is an alkaloid having odd number of -N- atom. The TR spectrum (KBr) indicates the present -OH, =CH, -CH, and C=C-C=O, secondary amine functional groups, in addition aromatic ring absorption band is also observed. Keywords: Fibraurea chloroleuca Miers., bioactive, BST, HeLa cell [Yogyakarta] : Universitas Gadjah Mada 2006 Article NonPeerReviewed Perpustakaan UGM, i-lib (2006) Skrining senyawa bioaktif dari beberapa ekstrak tumbuhan asal kawasan hutan Kalimantan Tengah dan isolasi senyawa bioaktif Fibraurea chloroleuca Miers = Bioactive compound screening from ... Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=37 |
| spellingShingle | Jurnal i-lib UGM Perpustakaan UGM, i-lib Skrining senyawa bioaktif dari beberapa ekstrak tumbuhan asal kawasan hutan Kalimantan Tengah dan isolasi senyawa bioaktif Fibraurea chloroleuca Miers = Bioactive compound screening from ... |
| title | Skrining senyawa bioaktif dari beberapa ekstrak tumbuhan asal kawasan hutan Kalimantan Tengah dan isolasi senyawa bioaktif Fibraurea chloroleuca Miers = Bioactive compound screening from ... |
| title_full | Skrining senyawa bioaktif dari beberapa ekstrak tumbuhan asal kawasan hutan Kalimantan Tengah dan isolasi senyawa bioaktif Fibraurea chloroleuca Miers = Bioactive compound screening from ... |
| title_fullStr | Skrining senyawa bioaktif dari beberapa ekstrak tumbuhan asal kawasan hutan Kalimantan Tengah dan isolasi senyawa bioaktif Fibraurea chloroleuca Miers = Bioactive compound screening from ... |
| title_full_unstemmed | Skrining senyawa bioaktif dari beberapa ekstrak tumbuhan asal kawasan hutan Kalimantan Tengah dan isolasi senyawa bioaktif Fibraurea chloroleuca Miers = Bioactive compound screening from ... |
| title_short | Skrining senyawa bioaktif dari beberapa ekstrak tumbuhan asal kawasan hutan Kalimantan Tengah dan isolasi senyawa bioaktif Fibraurea chloroleuca Miers = Bioactive compound screening from ... |
| title_sort | skrining senyawa bioaktif dari beberapa ekstrak tumbuhan asal kawasan hutan kalimantan tengah dan isolasi senyawa bioaktif fibraurea chloroleuca miers bioactive compound screening from |
| topic | Jurnal i-lib UGM |
| work_keys_str_mv | AT perpustakaanugmilib skriningsenyawabioaktifdaribeberapaekstraktumbuhanasalkawasanhutankalimantantengahdanisolasisenyawabioaktiffibraureachloroleucamiersbioactivecompoundscreeningfrom |