Karakterisasi Protease dan Isolat Bakteri Pendegradasi Tepung Bulu = Protease Characterization of Feathers Meal Degrading Bacteria Isolate
The objective of study was to characterize protease of degrading feather chicken isolate bacteria. Proteolytic bacteria isolated from the soil in the poultry slaughter waste area (B2 isolate) was used to produce enzyme at the temperature 50°C, pH 8 for 4 days. Crude enzyme obtained was presipited w...
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[Yogyakarta] : Universitas Gadjah Mada
2003
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| _version_ | 1797017597348151296 |
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| author | Perpustakaan UGM, i-lib |
| author_facet | Perpustakaan UGM, i-lib |
| author_sort | Perpustakaan UGM, i-lib |
| collection | UGM |
| description | The objective of study was to characterize protease of degrading feather chicken isolate bacteria. Proteolytic bacteria isolated from the soil in the poultry slaughter waste area (B2 isolate) was used to produce enzyme at the temperature 50°C, pH 8 for 4 days. Crude enzyme obtained was presipited with 70% saturated Ammonium Sulphate and dialyzed in phosphate buffer solution. The enzyme activity was characterized. The observation was done at the incubation temperature of 30, 33, 35, 37 and 40°C, medium pH at 6.0, 6.5, 7.0, 7.5 and 8.0, substrate concentration at 0.1, 0.25, 0.5, 0.75, 1.0, 1.25 and 1.5% and also time of incubation at 10, 20, 30, 40, 50 and 60 minutes. Kinetic parameters (Km and V.x values) were analyzed its optimum conditions. Highest protease spesific activity was observed at temperature of 35°C (0.085 U / mg prot), pH 7.0 (0.10 U /mg prot), substrate concentration of 1.25% ( 0.125 U / mg prot) and incubation time of 60 minutes ( 0.148 U /mg prot) (P |
| first_indexed | 2024-03-13T18:32:53Z |
| format | Article |
| id | oai:generic.eprints.org:17437 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T18:32:53Z |
| publishDate | 2003 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:174372014-06-18T00:30:11Z https://repository.ugm.ac.id/17437/ Karakterisasi Protease dan Isolat Bakteri Pendegradasi Tepung Bulu = Protease Characterization of Feathers Meal Degrading Bacteria Isolate Perpustakaan UGM, i-lib Jurnal i-lib UGM The objective of study was to characterize protease of degrading feather chicken isolate bacteria. Proteolytic bacteria isolated from the soil in the poultry slaughter waste area (B2 isolate) was used to produce enzyme at the temperature 50°C, pH 8 for 4 days. Crude enzyme obtained was presipited with 70% saturated Ammonium Sulphate and dialyzed in phosphate buffer solution. The enzyme activity was characterized. The observation was done at the incubation temperature of 30, 33, 35, 37 and 40°C, medium pH at 6.0, 6.5, 7.0, 7.5 and 8.0, substrate concentration at 0.1, 0.25, 0.5, 0.75, 1.0, 1.25 and 1.5% and also time of incubation at 10, 20, 30, 40, 50 and 60 minutes. Kinetic parameters (Km and V.x values) were analyzed its optimum conditions. Highest protease spesific activity was observed at temperature of 35°C (0.085 U / mg prot), pH 7.0 (0.10 U /mg prot), substrate concentration of 1.25% ( 0.125 U / mg prot) and incubation time of 60 minutes ( 0.148 U /mg prot) (P [Yogyakarta] : Universitas Gadjah Mada 2003 Article NonPeerReviewed Perpustakaan UGM, i-lib (2003) Karakterisasi Protease dan Isolat Bakteri Pendegradasi Tepung Bulu = Protease Characterization of Feathers Meal Degrading Bacteria Isolate. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=192 |
| spellingShingle | Jurnal i-lib UGM Perpustakaan UGM, i-lib Karakterisasi Protease dan Isolat Bakteri Pendegradasi Tepung Bulu = Protease Characterization of Feathers Meal Degrading Bacteria Isolate |
| title | Karakterisasi Protease dan Isolat Bakteri Pendegradasi Tepung Bulu = Protease Characterization of Feathers Meal Degrading Bacteria Isolate |
| title_full | Karakterisasi Protease dan Isolat Bakteri Pendegradasi Tepung Bulu = Protease Characterization of Feathers Meal Degrading Bacteria Isolate |
| title_fullStr | Karakterisasi Protease dan Isolat Bakteri Pendegradasi Tepung Bulu = Protease Characterization of Feathers Meal Degrading Bacteria Isolate |
| title_full_unstemmed | Karakterisasi Protease dan Isolat Bakteri Pendegradasi Tepung Bulu = Protease Characterization of Feathers Meal Degrading Bacteria Isolate |
| title_short | Karakterisasi Protease dan Isolat Bakteri Pendegradasi Tepung Bulu = Protease Characterization of Feathers Meal Degrading Bacteria Isolate |
| title_sort | karakterisasi protease dan isolat bakteri pendegradasi tepung bulu protease characterization of feathers meal degrading bacteria isolate |
| topic | Jurnal i-lib UGM |
| work_keys_str_mv | AT perpustakaanugmilib karakterisasiproteasedanisolatbakteripendegradasitepungbuluproteasecharacterizationoffeathersmealdegradingbacteriaisolate |