| Summary: | ABSTRAK
The objectives of studies were to obtain tungro monoclonal antibody for detecting virus occurrence in rice plant extract and to know the response of three rice varieties on the development of tungro in several growth development.
The preparation of tungro monoclonal antibody was done by fusion of lymphocyte BALB/C with NS1 myeloma. Immunogen immunization was done through spleen (intrasplenic) with dose of 40 (g/ml in 60 (10.01 M phosphate saline buffer, pH 7.4. The immunogen was purified RTV and its 260/280 absorbency ratio was about 1.45. At the beginning the hybrid cell formed was about 65, after passing three screening and two selection steps of cloning, four types of hybrid cell namely Ab-1, Ab-2, Ab-3, and Ab-4 were recovered. Pure RTV with 260/280 absorbancy ratio of 1.70 was used in selection and several antibody tests.
Final result of four hybrid cell were still heterogen clones of IgM and IgG2a antibodies. Optimal dilution for the Ab-3 antibody and that of antigen of disease plant extract were 10 and 100 times, respectively.
The observation of symptom and tungro virus propagation of three rice varieties for 8 weeks after inoculation showed that the disease symptom development and virus concentration were varied. The equal concentration of tungro virus on one variety resulted different disease severity on other varieties. The sensitivity to tungro infection of IR64 rice variety decreased at older stage, while on Mamberamo and Utri Merah varieties this sensitivity increased.
Keywords: monoclonal antibody - tungro virus detection - RTV concentration and symptom - rice variety response
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