Pengaruh kombinasi kadar sukrosa dan kalium nitrat dalam medium murashige skoog (ms) terhadapkadar atropina dan atau hiosiamina pada kultur kalus Datura stramonium L. var. stramonium =Combination Effect of Sucrose and ..
This research was aimed to study combination effect of sucrose and potasium nitrate concentration within Murashige-Skoog (MS) medium on producing biomass and'atropine/hyoscyamine contents within callus culture of Datura stramonium var. stramonium. In the research, explant of Datura stramonium v...
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[Yogyakarta] : Universitas Gadjah Mada
2004
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author | Perpustakaan UGM, i-lib |
author_facet | Perpustakaan UGM, i-lib |
author_sort | Perpustakaan UGM, i-lib |
collection | UGM |
description | This research was aimed to study combination effect of sucrose and potasium nitrate concentration within Murashige-Skoog (MS) medium on producing biomass and'atropine/hyoscyamine contents within callus culture of Datura stramonium var. stramonium.
In the research, explant of Datura stramonium var. stramonium leaves, planted on MS medium applying the addition of plant growth regulator 2,4-D and kinetin, each of 1 ppm. Medium for producing alkaloids applied similar medium to one for callus induction with addition of combination sucrose concentration (30, 35, 40 and 45 g/L) respectively and potasium nitrate (12.5%, 25%, 37.5%, and 50% based on standart content of MS medium).
The results suggested that MS medium added with 40 g/L sucrose and 37.5% potasium nitrate (S40I1375 medium) turned to be the most effective medium to produce biomass, while MS medium added with sucrose concentration of 40 g/L without the addition of potasium nitrate (S4oNo medium) was the most effective medium to produce atropine/ hyoscyamine.
Keywords : callus culture, atropine/hyoscyamine, Datura stramonium L. var. stramonium. |
first_indexed | 2024-03-05T22:52:17Z |
format | Article |
id | oai:generic.eprints.org:17586 |
institution | Universiti Gadjah Mada |
last_indexed | 2024-03-13T18:33:20Z |
publishDate | 2004 |
publisher | [Yogyakarta] : Universitas Gadjah Mada |
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spelling | oai:generic.eprints.org:175862014-06-18T00:28:51Z https://repository.ugm.ac.id/17586/ Pengaruh kombinasi kadar sukrosa dan kalium nitrat dalam medium murashige skoog (ms) terhadapkadar atropina dan atau hiosiamina pada kultur kalus Datura stramonium L. var. stramonium =Combination Effect of Sucrose and .. Perpustakaan UGM, i-lib Jurnal i-lib UGM This research was aimed to study combination effect of sucrose and potasium nitrate concentration within Murashige-Skoog (MS) medium on producing biomass and'atropine/hyoscyamine contents within callus culture of Datura stramonium var. stramonium. In the research, explant of Datura stramonium var. stramonium leaves, planted on MS medium applying the addition of plant growth regulator 2,4-D and kinetin, each of 1 ppm. Medium for producing alkaloids applied similar medium to one for callus induction with addition of combination sucrose concentration (30, 35, 40 and 45 g/L) respectively and potasium nitrate (12.5%, 25%, 37.5%, and 50% based on standart content of MS medium). The results suggested that MS medium added with 40 g/L sucrose and 37.5% potasium nitrate (S40I1375 medium) turned to be the most effective medium to produce biomass, while MS medium added with sucrose concentration of 40 g/L without the addition of potasium nitrate (S4oNo medium) was the most effective medium to produce atropine/ hyoscyamine. Keywords : callus culture, atropine/hyoscyamine, Datura stramonium L. var. stramonium. [Yogyakarta] : Universitas Gadjah Mada 2004 Article NonPeerReviewed Perpustakaan UGM, i-lib (2004) Pengaruh kombinasi kadar sukrosa dan kalium nitrat dalam medium murashige skoog (ms) terhadapkadar atropina dan atau hiosiamina pada kultur kalus Datura stramonium L. var. stramonium =Combination Effect of Sucrose and .. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=345 |
spellingShingle | Jurnal i-lib UGM Perpustakaan UGM, i-lib Pengaruh kombinasi kadar sukrosa dan kalium nitrat dalam medium murashige skoog (ms) terhadapkadar atropina dan atau hiosiamina pada kultur kalus Datura stramonium L. var. stramonium =Combination Effect of Sucrose and .. |
title | Pengaruh kombinasi kadar sukrosa dan kalium nitrat dalam medium murashige skoog (ms) terhadapkadar atropina dan atau hiosiamina pada kultur kalus Datura stramonium L. var. stramonium =Combination Effect of Sucrose and .. |
title_full | Pengaruh kombinasi kadar sukrosa dan kalium nitrat dalam medium murashige skoog (ms) terhadapkadar atropina dan atau hiosiamina pada kultur kalus Datura stramonium L. var. stramonium =Combination Effect of Sucrose and .. |
title_fullStr | Pengaruh kombinasi kadar sukrosa dan kalium nitrat dalam medium murashige skoog (ms) terhadapkadar atropina dan atau hiosiamina pada kultur kalus Datura stramonium L. var. stramonium =Combination Effect of Sucrose and .. |
title_full_unstemmed | Pengaruh kombinasi kadar sukrosa dan kalium nitrat dalam medium murashige skoog (ms) terhadapkadar atropina dan atau hiosiamina pada kultur kalus Datura stramonium L. var. stramonium =Combination Effect of Sucrose and .. |
title_short | Pengaruh kombinasi kadar sukrosa dan kalium nitrat dalam medium murashige skoog (ms) terhadapkadar atropina dan atau hiosiamina pada kultur kalus Datura stramonium L. var. stramonium =Combination Effect of Sucrose and .. |
title_sort | pengaruh kombinasi kadar sukrosa dan kalium nitrat dalam medium murashige skoog ms terhadapkadar atropina dan atau hiosiamina pada kultur kalus datura stramonium l var stramonium combination effect of sucrose and |
topic | Jurnal i-lib UGM |
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