Xylan degrading enzymes of the yeast Candida tropicalis strain R1
Yeast, Candida tropicalis strain R1, was isolated from Indonesian ragi by selective enrichment for xylan degradation. Objective of the current research was to elucidate the characteristic of xylanases produced by the strain. Sugar cane-bagasse xylans were used as a growth substrate of this strain. B...
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[Yogyakarta] : Universitas Gadjah Mada
1998
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author | Perpustakaan UGM, i-lib |
author_facet | Perpustakaan UGM, i-lib |
author_sort | Perpustakaan UGM, i-lib |
collection | UGM |
description | Yeast, Candida tropicalis strain R1, was isolated from Indonesian ragi by selective enrichment for xylan degradation. Objective of the current research was to elucidate the characteristic of xylanases produced by the strain. Sugar cane-bagasse xylans were used as a growth substrate of this strain. Biodegradation of xylan was monitored by measuring oligosaccharides and xyloses released into the medium and visualised using thin layer chromatography. The growth of Candida tropicalis strain R1 on bagasse xylans yielded two enzymes that converted xylans to xyloses or to oligosaccharides. Those enzymes almost completely released into culture fluid were identified as b-xylanase and xylosidase. The extracellular b-xylanases hydrolysed xylans to xylooligomers, such as xylotetraose, xylotriose and xylobiose. The other route of xylan catabolism was inside the cell. This catabolism involved the second cell membrane bound enzyme called b-xylosidase which split the xylans into both xyloses and xylooligomers that were further fermented to ethanol by this strain.
Both of those enzymes were produced in lower concentration by cells grown on glucose than by those grown on xylan. It assumed that those enzymes were inducible enzymes. Candida tropicalis strain R-1 was able to assimilate and ferment xylooligomers to produce ethanol. It seemed that cell growth and ethanol productions were directly proportional to xylooligomer concentration. This strain was unable to hydrolyse cellulose.
Keywords: biodegradation -- xylan xylooligomers xylanases xylosidases |
first_indexed | 2024-03-05T22:52:31Z |
format | Article |
id | oai:generic.eprints.org:17709 |
institution | Universiti Gadjah Mada |
last_indexed | 2024-03-13T18:33:43Z |
publishDate | 1998 |
publisher | [Yogyakarta] : Universitas Gadjah Mada |
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spelling | oai:generic.eprints.org:177092014-06-18T00:37:34Z https://repository.ugm.ac.id/17709/ Xylan degrading enzymes of the yeast Candida tropicalis strain R1 Perpustakaan UGM, i-lib Jurnal i-lib UGM Yeast, Candida tropicalis strain R1, was isolated from Indonesian ragi by selective enrichment for xylan degradation. Objective of the current research was to elucidate the characteristic of xylanases produced by the strain. Sugar cane-bagasse xylans were used as a growth substrate of this strain. Biodegradation of xylan was monitored by measuring oligosaccharides and xyloses released into the medium and visualised using thin layer chromatography. The growth of Candida tropicalis strain R1 on bagasse xylans yielded two enzymes that converted xylans to xyloses or to oligosaccharides. Those enzymes almost completely released into culture fluid were identified as b-xylanase and xylosidase. The extracellular b-xylanases hydrolysed xylans to xylooligomers, such as xylotetraose, xylotriose and xylobiose. The other route of xylan catabolism was inside the cell. This catabolism involved the second cell membrane bound enzyme called b-xylosidase which split the xylans into both xyloses and xylooligomers that were further fermented to ethanol by this strain. Both of those enzymes were produced in lower concentration by cells grown on glucose than by those grown on xylan. It assumed that those enzymes were inducible enzymes. Candida tropicalis strain R-1 was able to assimilate and ferment xylooligomers to produce ethanol. It seemed that cell growth and ethanol productions were directly proportional to xylooligomer concentration. This strain was unable to hydrolyse cellulose. Keywords: biodegradation -- xylan xylooligomers xylanases xylosidases [Yogyakarta] : Universitas Gadjah Mada 1998 Article NonPeerReviewed Perpustakaan UGM, i-lib (1998) Xylan degrading enzymes of the yeast Candida tropicalis strain R1. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=472 |
spellingShingle | Jurnal i-lib UGM Perpustakaan UGM, i-lib Xylan degrading enzymes of the yeast Candida tropicalis strain R1 |
title | Xylan degrading enzymes of the yeast Candida tropicalis strain R1 |
title_full | Xylan degrading enzymes of the yeast Candida tropicalis strain R1 |
title_fullStr | Xylan degrading enzymes of the yeast Candida tropicalis strain R1 |
title_full_unstemmed | Xylan degrading enzymes of the yeast Candida tropicalis strain R1 |
title_short | Xylan degrading enzymes of the yeast Candida tropicalis strain R1 |
title_sort | xylan degrading enzymes of the yeast candida tropicalis strain r1 |
topic | Jurnal i-lib UGM |
work_keys_str_mv | AT perpustakaanugmilib xylandegradingenzymesoftheyeastcandidatropicalisstrainr1 |