An ELISA-based detection of Porphyromonas gingivalis in the dental plaques of periodontal diseased patients
Murine hybriden:as producing lipopolisaccharides of Porphyromonas gingivalis (LPS-Pg)-specific monoclonal antibodies were established. Dental plaques were obtained from periodontal healthy subjects and patients with gingivitis or periodontitis. The levels of P. gingivalis were semiquantitatively det...
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| Format: | Article |
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[Yogyakarta] : Universitas Gadjah Mada
1999
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| author | Perpustakaan UGM, i-lib |
| author_facet | Perpustakaan UGM, i-lib |
| author_sort | Perpustakaan UGM, i-lib |
| collection | UGM |
| description | Murine hybriden:as producing lipopolisaccharides of Porphyromonas gingivalis (LPS-Pg)-specific monoclonal antibodies were established. Dental plaques were obtained from periodontal healthy subjects and patients with gingivitis or periodontitis. The levels of P. gingivalis were semiquantitatively detected by ELISA. The results showed that antibodies produced by clone Pg.C.5 were lgG2a and recognized specifically LPS-Pg, but not LPS from other periodontopathic or enteric bacteria. The presence of P. gingivalis in the dental plaque of periodontal diseased patients was able to be detected by ELISA using these antibodies. The levels of this bacterium in patients with periodontitis were much higher than those of patients with gingivitis or periodontal healthy subjects (p |
| first_indexed | 2024-03-13T18:34:07Z |
| format | Article |
| id | oai:generic.eprints.org:17840 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T18:34:07Z |
| publishDate | 1999 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:178402014-06-18T00:35:03Z https://repository.ugm.ac.id/17840/ An ELISA-based detection of Porphyromonas gingivalis in the dental plaques of periodontal diseased patients Perpustakaan UGM, i-lib Jurnal i-lib UGM Murine hybriden:as producing lipopolisaccharides of Porphyromonas gingivalis (LPS-Pg)-specific monoclonal antibodies were established. Dental plaques were obtained from periodontal healthy subjects and patients with gingivitis or periodontitis. The levels of P. gingivalis were semiquantitatively detected by ELISA. The results showed that antibodies produced by clone Pg.C.5 were lgG2a and recognized specifically LPS-Pg, but not LPS from other periodontopathic or enteric bacteria. The presence of P. gingivalis in the dental plaque of periodontal diseased patients was able to be detected by ELISA using these antibodies. The levels of this bacterium in patients with periodontitis were much higher than those of patients with gingivitis or periodontal healthy subjects (p [Yogyakarta] : Universitas Gadjah Mada 1999 Article NonPeerReviewed Perpustakaan UGM, i-lib (1999) An ELISA-based detection of Porphyromonas gingivalis in the dental plaques of periodontal diseased patients. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=608 |
| spellingShingle | Jurnal i-lib UGM Perpustakaan UGM, i-lib An ELISA-based detection of Porphyromonas gingivalis in the dental plaques of periodontal diseased patients |
| title | An ELISA-based detection of Porphyromonas gingivalis in the dental plaques of periodontal diseased patients |
| title_full | An ELISA-based detection of Porphyromonas gingivalis in the dental plaques of periodontal diseased patients |
| title_fullStr | An ELISA-based detection of Porphyromonas gingivalis in the dental plaques of periodontal diseased patients |
| title_full_unstemmed | An ELISA-based detection of Porphyromonas gingivalis in the dental plaques of periodontal diseased patients |
| title_short | An ELISA-based detection of Porphyromonas gingivalis in the dental plaques of periodontal diseased patients |
| title_sort | elisa based detection of porphyromonas gingivalis in the dental plaques of periodontal diseased patients |
| topic | Jurnal i-lib UGM |
| work_keys_str_mv | AT perpustakaanugmilib anelisabaseddetectionofporphyromonasgingivalisinthedentalplaquesofperiodontaldiseasedpatients AT perpustakaanugmilib elisabaseddetectionofporphyromonasgingivalisinthedentalplaquesofperiodontaldiseasedpatients |