Summary: | Identification of 2,4-D-degrading bacteria isolated from corals was carried out based on restriction analysis. Five endonucleases (Hhal, HaeIII, Sau3 AI, Rsal and MspI) were used to digest PCR-amplified 16S rDNA of these bacteria, and produced several informative bands. Comparing all the restriction patterns among four 2,4-D-degrading bacteria, two genotypes (I and II) were obtained. Based on 16S rDNA sequence analysis, those bacteria were identified as Vibrio natriegens and Vibrio alginilyticus.
Keywords: 2,4-D-degrading bacteria � 16S rDNA � PCR � RFLP � phylogenetic
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