Restriction fragment length polymorphism analysis of PCR-amplified 16S rDNA of 2.4-D-degrading bacteria isolated form corals
Identification of 2,4-D-degrading bacteria isolated from corals was carried out based on restriction analysis. Five endonucleases (Hhal, HaeIII, Sau3 AI, Rsal and MspI) were used to digest PCR-amplified 16S rDNA of these bacteria, and produced several informative bands. Comparing all the restriction...
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[Yogyakarta] : Universitas Gadjah Mada
2000
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| _version_ | 1797017735912226816 |
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| author | Perpustakaan UGM, i-lib |
| author_facet | Perpustakaan UGM, i-lib |
| author_sort | Perpustakaan UGM, i-lib |
| collection | UGM |
| description | Identification of 2,4-D-degrading bacteria isolated from corals was carried out based on restriction analysis. Five endonucleases (Hhal, HaeIII, Sau3 AI, Rsal and MspI) were used to digest PCR-amplified 16S rDNA of these bacteria, and produced several informative bands. Comparing all the restriction patterns among four 2,4-D-degrading bacteria, two genotypes (I and II) were obtained. Based on 16S rDNA sequence analysis, those bacteria were identified as Vibrio natriegens and Vibrio alginilyticus.
Keywords: 2,4-D-degrading bacteria � 16S rDNA � PCR � RFLP � phylogenetic |
| first_indexed | 2024-03-05T22:53:05Z |
| format | Article |
| id | oai:generic.eprints.org:18117 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T18:35:01Z |
| publishDate | 2000 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:181172014-06-18T00:34:31Z https://repository.ugm.ac.id/18117/ Restriction fragment length polymorphism analysis of PCR-amplified 16S rDNA of 2.4-D-degrading bacteria isolated form corals Perpustakaan UGM, i-lib Jurnal i-lib UGM Identification of 2,4-D-degrading bacteria isolated from corals was carried out based on restriction analysis. Five endonucleases (Hhal, HaeIII, Sau3 AI, Rsal and MspI) were used to digest PCR-amplified 16S rDNA of these bacteria, and produced several informative bands. Comparing all the restriction patterns among four 2,4-D-degrading bacteria, two genotypes (I and II) were obtained. Based on 16S rDNA sequence analysis, those bacteria were identified as Vibrio natriegens and Vibrio alginilyticus. Keywords: 2,4-D-degrading bacteria � 16S rDNA � PCR � RFLP � phylogenetic [Yogyakarta] : Universitas Gadjah Mada 2000 Article NonPeerReviewed Perpustakaan UGM, i-lib (2000) Restriction fragment length polymorphism analysis of PCR-amplified 16S rDNA of 2.4-D-degrading bacteria isolated form corals. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=895 |
| spellingShingle | Jurnal i-lib UGM Perpustakaan UGM, i-lib Restriction fragment length polymorphism analysis of PCR-amplified 16S rDNA of 2.4-D-degrading bacteria isolated form corals |
| title | Restriction fragment length polymorphism analysis of PCR-amplified 16S rDNA of 2.4-D-degrading bacteria isolated form corals |
| title_full | Restriction fragment length polymorphism analysis of PCR-amplified 16S rDNA of 2.4-D-degrading bacteria isolated form corals |
| title_fullStr | Restriction fragment length polymorphism analysis of PCR-amplified 16S rDNA of 2.4-D-degrading bacteria isolated form corals |
| title_full_unstemmed | Restriction fragment length polymorphism analysis of PCR-amplified 16S rDNA of 2.4-D-degrading bacteria isolated form corals |
| title_short | Restriction fragment length polymorphism analysis of PCR-amplified 16S rDNA of 2.4-D-degrading bacteria isolated form corals |
| title_sort | restriction fragment length polymorphism analysis of pcr amplified 16s rdna of 2 4 d degrading bacteria isolated form corals |
| topic | Jurnal i-lib UGM |
| work_keys_str_mv | AT perpustakaanugmilib restrictionfragmentlengthpolymorphismanalysisofpcramplified16srdnaof24ddegradingbacteriaisolatedformcorals |