| Summary: | Infectious Mononucleosis (IM) is a viral disease caused by Epstein-Barr Virus (EBV). The incidence of IM is low in Indonesia. EBV latent infection on lymphocytes undergoes changes in proliferation and differentiation characters. Transcription product of c-myc will lead lymphocytes to become proliferation or apoptosis, whereas bc1-2 gene product, a transmembrane protein has some role as an anti-apoptosis. Proliferative and anti-apoptosis interaction will act as the major components in the onset of carcinogenesis.
The objective of this study is to determine semi-quantitatively the degree of bc1-2 and c-myc gene expression of peripheral blood lymphocytes from IM patients as compared to non-diagnostic-asymptomatic (NDA) persons. Five IM and five NDA individuals were used in this study. Lymphocyte and blood serum were separated using Ficoll-Histopaque Gradient Method, Antibody titer of IgA anti EBNA and IgA anti VCA were determined using ELISA Kit for anti EBNA and anti VCA (PanBio, Australia). Total. RNA was extracted by Acid Guanidine Isothiocyanat. Probe of bcl-2 and c-myc were biotin labeled. The degree of bc1-2 and c-myc gene product were analyzed by blotting, hybridized and detected using Tyramide System AmplificationTm-Indirect (ISH) (NENTM Life Science Products, USA), and examined under TLC Scanner.
It was shown in this experiment that IM patients have dominantly higher bc1-2 and c-myc gene expression product compared to NDA individuals. The technique used in this experiment could be applied to differentiate IM and NDA individuals by analyzing the degree of bc1-2 and c-myc genes expression products.
Keywords: bc1-2 � c-myc � Epstein-Barr Virus � IM � apoptosis
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