Application of non-specific esterase enzyme microassays to detect potential insecticide resistance of Aedes aegypti adults in Yogyakarta, Indonesia
ABSTRAK Dengan bioesai larvae Aedes aegypti yang dikumpulkan dari wilayah Yogyakarta ternyata masih rentan terhadap temefos dan malathion dan dengan uji mikroplat terbukti juga Ae. aegypti dart lokasi yang sama berpotensi resisten terhadap insektisida karena adanya peningkatan aktivitas enzim ester...
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[Yogyakarta] : Universitas Gadjah Mada
1996
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| _version_ | 1797017756083683328 |
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| author | Perpustakaan UGM, i-lib |
| author_facet | Perpustakaan UGM, i-lib |
| author_sort | Perpustakaan UGM, i-lib |
| collection | UGM |
| description | ABSTRAK
Dengan bioesai larvae Aedes aegypti yang dikumpulkan dari wilayah Yogyakarta ternyata masih rentan terhadap temefos dan malathion dan dengan uji mikroplat terbukti juga Ae. aegypti dart lokasi yang sama berpotensi resisten terhadap insektisida karena adanya peningkatan aktivitas enzim esterase. Karena itu, Dengan bioesai larvae Aedes aegypti yang dikumpulkan dart wilayah Yogyakarta ternyata masih rentan timbul dugaan bahwa nyamuk dewasa Ae. aegypti di Yogyakarta juga berpotensi resisten terhadap senyawa organofosfat 10P) karena mekanisme resistensi yang sama. Penelitian ini bertujuan untuk mendeteksi potensi resistensi insektisida OP pada nyamuk Ae. aegypti di Yogyakarta yang berkaitan dengan aktivitas enzim esterase non-spesifik. Cara penelitian meliputi penggunaan mikroesai untuk peningkatan enzim esterase dengan substrat a-naphthyl acetate pada Ae. aegypti stadium dewasa asal koleksi lapangan dibandingkan dengan yang koloni laboratorium. Dart rangkaian uji biokimia enzimatik itu terbukti bahwa nyamuk Ae. aegypti di Yogyakarta praktis masih rentan dan berpotensi menjadi resisten terhadap insektiinsektisida yang berkaitan dengan aktivitas enzim esterase non-spesifik yang mampu menghidrolisis a-naphthyl acetate.
By bioassay Aedes aegypti larvae collected from Yogyakarta were found to be susceptible to temephos and malathion and by using microplate enzymatic assay Ae. aegypti larvae collected from the same sites were potentially resistant to the organophosphate insecticides due to elevated esterase activity in hydrolyzing a-naphthyl acetate substrate used in the enzymatic reaction from the same sites might be potentially resistant to OP insecticides due to the same mechanism. The homogenates of the mosquito adult stages from the fields were microassayed using a-naphthyl acetate substrate compared to that colonized in the laboratory. From a series of studies the results were concluded that Ae. aegypti adults in Yogyakarta were susceptible and potentially resistant to insecticides due to elevated a-naphthyl acetate esterase activity.
Key words: Aedes aegypti - organophosphate insecticides - insecticide resistance - non-spesific esterase -biochemical test. |
| first_indexed | 2024-03-05T22:53:15Z |
| format | Article |
| id | oai:generic.eprints.org:18214 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T18:35:19Z |
| publishDate | 1996 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:182142014-06-18T00:38:25Z https://repository.ugm.ac.id/18214/ Application of non-specific esterase enzyme microassays to detect potential insecticide resistance of Aedes aegypti adults in Yogyakarta, Indonesia Perpustakaan UGM, i-lib Jurnal i-lib UGM ABSTRAK Dengan bioesai larvae Aedes aegypti yang dikumpulkan dari wilayah Yogyakarta ternyata masih rentan terhadap temefos dan malathion dan dengan uji mikroplat terbukti juga Ae. aegypti dart lokasi yang sama berpotensi resisten terhadap insektisida karena adanya peningkatan aktivitas enzim esterase. Karena itu, Dengan bioesai larvae Aedes aegypti yang dikumpulkan dart wilayah Yogyakarta ternyata masih rentan timbul dugaan bahwa nyamuk dewasa Ae. aegypti di Yogyakarta juga berpotensi resisten terhadap senyawa organofosfat 10P) karena mekanisme resistensi yang sama. Penelitian ini bertujuan untuk mendeteksi potensi resistensi insektisida OP pada nyamuk Ae. aegypti di Yogyakarta yang berkaitan dengan aktivitas enzim esterase non-spesifik. Cara penelitian meliputi penggunaan mikroesai untuk peningkatan enzim esterase dengan substrat a-naphthyl acetate pada Ae. aegypti stadium dewasa asal koleksi lapangan dibandingkan dengan yang koloni laboratorium. Dart rangkaian uji biokimia enzimatik itu terbukti bahwa nyamuk Ae. aegypti di Yogyakarta praktis masih rentan dan berpotensi menjadi resisten terhadap insektiinsektisida yang berkaitan dengan aktivitas enzim esterase non-spesifik yang mampu menghidrolisis a-naphthyl acetate. By bioassay Aedes aegypti larvae collected from Yogyakarta were found to be susceptible to temephos and malathion and by using microplate enzymatic assay Ae. aegypti larvae collected from the same sites were potentially resistant to the organophosphate insecticides due to elevated esterase activity in hydrolyzing a-naphthyl acetate substrate used in the enzymatic reaction from the same sites might be potentially resistant to OP insecticides due to the same mechanism. The homogenates of the mosquito adult stages from the fields were microassayed using a-naphthyl acetate substrate compared to that colonized in the laboratory. From a series of studies the results were concluded that Ae. aegypti adults in Yogyakarta were susceptible and potentially resistant to insecticides due to elevated a-naphthyl acetate esterase activity. Key words: Aedes aegypti - organophosphate insecticides - insecticide resistance - non-spesific esterase -biochemical test. [Yogyakarta] : Universitas Gadjah Mada 1996 Article NonPeerReviewed Perpustakaan UGM, i-lib (1996) Application of non-specific esterase enzyme microassays to detect potential insecticide resistance of Aedes aegypti adults in Yogyakarta, Indonesia. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=992 |
| spellingShingle | Jurnal i-lib UGM Perpustakaan UGM, i-lib Application of non-specific esterase enzyme microassays to detect potential insecticide resistance of Aedes aegypti adults in Yogyakarta, Indonesia |
| title | Application of non-specific esterase enzyme microassays to detect potential insecticide resistance of Aedes aegypti adults in Yogyakarta, Indonesia |
| title_full | Application of non-specific esterase enzyme microassays to detect potential insecticide resistance of Aedes aegypti adults in Yogyakarta, Indonesia |
| title_fullStr | Application of non-specific esterase enzyme microassays to detect potential insecticide resistance of Aedes aegypti adults in Yogyakarta, Indonesia |
| title_full_unstemmed | Application of non-specific esterase enzyme microassays to detect potential insecticide resistance of Aedes aegypti adults in Yogyakarta, Indonesia |
| title_short | Application of non-specific esterase enzyme microassays to detect potential insecticide resistance of Aedes aegypti adults in Yogyakarta, Indonesia |
| title_sort | application of non specific esterase enzyme microassays to detect potential insecticide resistance of aedes aegypti adults in yogyakarta indonesia |
| topic | Jurnal i-lib UGM |
| work_keys_str_mv | AT perpustakaanugmilib applicationofnonspecificesteraseenzymemicroassaystodetectpotentialinsecticideresistanceofaedesaegyptiadultsinyogyakartaindonesia |