| Summary: | Immunoglubolin G (IgG) binding proteins on the surface of Streptococcus suis could be solubilized by heat treatment of the bacteria at an acid pH and also by mutanolysin treatment. The IgG binding proteins could be detected by Western blot using human IgG and peroxidase-labelled anti-human, and autoradiography analysis using 125I-labelled human IgG. Western blot and autoradiography analysis revealed numerous protein bands with IgG binding activities. In binding studies using Western blot and autoradipgraphy, S. suis P43, S. suis P143 and S. suis 2872, but none of the other S. suis isolates, showed a significant binding of the protein. The IgG binding proteins were also released into the culture supernatant of the bacteria. This could be detected for 52 of the 76 S. suis isolates.
Keywords: Streptococcus suis immunoglobulin G - binding protein
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