Deteksi Dini Antigen Beredar Toxoplasma Gondii Pada Manusia Dan Hewan Dengan Menggunakan Antibodi Monoklonal
ABSTRAK The aim of this study were to detect circulating antigen of Toxoplasma gondii using 129 monoclonal antibody to membrane antigen of local isolated Toxoplasma gondii as an early detection for toxoplasmosis and to know the prevalence of toxoplasmosis of slaughterhouse in Yogyakarta district. In...
| Main Author: | |
|---|---|
| Format: | Article |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
1998
|
| Subjects: |
| _version_ | 1797017809407967232 |
|---|---|
| author | Perpustakaan UGM, i-lib |
| author_facet | Perpustakaan UGM, i-lib |
| author_sort | Perpustakaan UGM, i-lib |
| collection | UGM |
| description | ABSTRAK
The aim of this study were to detect circulating antigen of Toxoplasma gondii using 129 monoclonal antibody to membrane antigen of local isolated Toxoplasma gondii as an early detection for toxoplasmosis and to know the prevalence of toxoplasmosis of slaughterhouse in Yogyakarta district.
In this study the specimens from cows (80 specimens), sheep (80 specimens), and pregnant women (20 specimens) were used respectively. The samples were tested for toxoplasmosis using ELISA Sandwich Test. Checkerboard was done before ELISA Sandwich Test to determine antibody dilution and antibody choice. Sample from pregnant women which has been tested by commercial serology, that is Toxo ISAGA and Toxo Screen DA, were then tested by ELISA Sandwich Test. The comparison of Toxo ISAGA, Toxo Screen DA, and ELISA Sandwich results was used to determine sensitivity test.
The result showed that the use of 129 monoclonal antibody to membrane antigen of local isolated Toxoplasma gondii had 88.8% sensitivity. The prevalence of toxoplasmosis was 27.5% in cow and 76.25% in sheep. It could be summarized that 129 monoclonal antibody of local isolated Toxoplasma gondii might be used to detect circulating antigen of toxoplasmosis cases in the early stage |
| first_indexed | 2024-03-05T22:53:32Z |
| format | Article |
| id | oai:generic.eprints.org:18393 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T18:35:52Z |
| publishDate | 1998 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:183932014-06-18T00:36:46Z https://repository.ugm.ac.id/18393/ Deteksi Dini Antigen Beredar Toxoplasma Gondii Pada Manusia Dan Hewan Dengan Menggunakan Antibodi Monoklonal Perpustakaan UGM, i-lib Jurnal i-lib UGM ABSTRAK The aim of this study were to detect circulating antigen of Toxoplasma gondii using 129 monoclonal antibody to membrane antigen of local isolated Toxoplasma gondii as an early detection for toxoplasmosis and to know the prevalence of toxoplasmosis of slaughterhouse in Yogyakarta district. In this study the specimens from cows (80 specimens), sheep (80 specimens), and pregnant women (20 specimens) were used respectively. The samples were tested for toxoplasmosis using ELISA Sandwich Test. Checkerboard was done before ELISA Sandwich Test to determine antibody dilution and antibody choice. Sample from pregnant women which has been tested by commercial serology, that is Toxo ISAGA and Toxo Screen DA, were then tested by ELISA Sandwich Test. The comparison of Toxo ISAGA, Toxo Screen DA, and ELISA Sandwich results was used to determine sensitivity test. The result showed that the use of 129 monoclonal antibody to membrane antigen of local isolated Toxoplasma gondii had 88.8% sensitivity. The prevalence of toxoplasmosis was 27.5% in cow and 76.25% in sheep. It could be summarized that 129 monoclonal antibody of local isolated Toxoplasma gondii might be used to detect circulating antigen of toxoplasmosis cases in the early stage [Yogyakarta] : Universitas Gadjah Mada 1998 Article NonPeerReviewed Perpustakaan UGM, i-lib (1998) Deteksi Dini Antigen Beredar Toxoplasma Gondii Pada Manusia Dan Hewan Dengan Menggunakan Antibodi Monoklonal. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=1178 |
| spellingShingle | Jurnal i-lib UGM Perpustakaan UGM, i-lib Deteksi Dini Antigen Beredar Toxoplasma Gondii Pada Manusia Dan Hewan Dengan Menggunakan Antibodi Monoklonal |
| title | Deteksi Dini Antigen Beredar Toxoplasma Gondii Pada Manusia Dan Hewan Dengan Menggunakan Antibodi Monoklonal |
| title_full | Deteksi Dini Antigen Beredar Toxoplasma Gondii Pada Manusia Dan Hewan Dengan Menggunakan Antibodi Monoklonal |
| title_fullStr | Deteksi Dini Antigen Beredar Toxoplasma Gondii Pada Manusia Dan Hewan Dengan Menggunakan Antibodi Monoklonal |
| title_full_unstemmed | Deteksi Dini Antigen Beredar Toxoplasma Gondii Pada Manusia Dan Hewan Dengan Menggunakan Antibodi Monoklonal |
| title_short | Deteksi Dini Antigen Beredar Toxoplasma Gondii Pada Manusia Dan Hewan Dengan Menggunakan Antibodi Monoklonal |
| title_sort | deteksi dini antigen beredar toxoplasma gondii pada manusia dan hewan dengan menggunakan antibodi monoklonal |
| topic | Jurnal i-lib UGM |
| work_keys_str_mv | AT perpustakaanugmilib deteksidiniantigenberedartoxoplasmagondiipadamanusiadanhewandenganmenggunakanantibodimonoklonal |