A Simplified Method for Determination of Free Fatty Acids for Soluble and Immobilized Lipase Assay
ABSTRACT A simple and rapid method for determination of free fatty acids for soluble and immobilized lipase assay was developed. The free fatty acids could be determined within 10 min with less organic solvent used and the color developed was stable until 60 min. High correlation (r > 0.97) betwe...
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| Format: | Article |
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[Yogyakarta] : Universitas Gadjah Mada
1998
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| _version_ | 1797018015215124480 |
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| author | Perpustakaan UGM, i-lib |
| author_facet | Perpustakaan UGM, i-lib |
| author_sort | Perpustakaan UGM, i-lib |
| collection | UGM |
| description | ABSTRACT
A simple and rapid method for determination of free fatty acids for soluble and immobilized lipase assay was developed. The free fatty acids could be determined within 10 min with less organic solvent used and the color developed was stable until 60 min. High correlation (r > 0.97) between fatty acids content (2 -10 pmole) and absorbance was observed for fatty acids with carbon number of 6 or higher. Hydrolysis activity of soluble and immobilized lipase could be measured with high sensitivity and reproducibility against incubation time and protein loading. The effect of various substrate concentrations and water against hydrolysis activity could also be measured. The method was suitable for routine analysis such as purification of lipase and continuous hydrolysis of fat and oil. |
| first_indexed | 2024-03-05T22:55:29Z |
| format | Article |
| id | oai:generic.eprints.org:19402 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T18:37:48Z |
| publishDate | 1998 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:194022014-06-18T00:37:31Z https://repository.ugm.ac.id/19402/ A Simplified Method for Determination of Free Fatty Acids for Soluble and Immobilized Lipase Assay Perpustakaan UGM, i-lib Jurnal i-lib UGM ABSTRACT A simple and rapid method for determination of free fatty acids for soluble and immobilized lipase assay was developed. The free fatty acids could be determined within 10 min with less organic solvent used and the color developed was stable until 60 min. High correlation (r > 0.97) between fatty acids content (2 -10 pmole) and absorbance was observed for fatty acids with carbon number of 6 or higher. Hydrolysis activity of soluble and immobilized lipase could be measured with high sensitivity and reproducibility against incubation time and protein loading. The effect of various substrate concentrations and water against hydrolysis activity could also be measured. The method was suitable for routine analysis such as purification of lipase and continuous hydrolysis of fat and oil. [Yogyakarta] : Universitas Gadjah Mada 1998 Article NonPeerReviewed Perpustakaan UGM, i-lib (1998) A Simplified Method for Determination of Free Fatty Acids for Soluble and Immobilized Lipase Assay. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=2232 |
| spellingShingle | Jurnal i-lib UGM Perpustakaan UGM, i-lib A Simplified Method for Determination of Free Fatty Acids for Soluble and Immobilized Lipase Assay |
| title | A Simplified Method for Determination of Free Fatty Acids for Soluble and Immobilized Lipase Assay |
| title_full | A Simplified Method for Determination of Free Fatty Acids for Soluble and Immobilized Lipase Assay |
| title_fullStr | A Simplified Method for Determination of Free Fatty Acids for Soluble and Immobilized Lipase Assay |
| title_full_unstemmed | A Simplified Method for Determination of Free Fatty Acids for Soluble and Immobilized Lipase Assay |
| title_short | A Simplified Method for Determination of Free Fatty Acids for Soluble and Immobilized Lipase Assay |
| title_sort | simplified method for determination of free fatty acids for soluble and immobilized lipase assay |
| topic | Jurnal i-lib UGM |
| work_keys_str_mv | AT perpustakaanugmilib asimplifiedmethodfordeterminationoffreefattyacidsforsolubleandimmobilizedlipaseassay AT perpustakaanugmilib simplifiedmethodfordeterminationoffreefattyacidsforsolubleandimmobilizedlipaseassay |