| Summary: | ABSTRACT
Dengue virus infection can produce severe clinical manifestiations such as dengue haemorrhagic fever (DHF) and the dengue shock syndrome (DSS). Mechanisms underlying the disease i.e. pathogenesis, hemodynamics and biochemical aspects are not clearly understood. This are due to the lack of experimental animals as a model to study such a disease in human. Plasma leakage from capillaries which increased their permeability suggests that endothelial cells may play a role in the pathogenesis of DHF and / or DSS.
The study was aimed at investigating the kinetics of TNF-a production by endothelial cell culture after being infected with dengue virus serotype-2 and-3.
Endothelial cells collected from human umbilical veins were cultured on a 24 well plate, and were then exposed to dengue virus serotype-2 and-3 with MOI-1. The levelof TNF-a on culture supernatant was detected using pelikin human TNF-a ELISA kit, and the kinetics of their production were observed at 30,60,90 minutes, and 2,3,18, 24,48, 72,96 hours after infection.
The results demonstrated that both serotypes induce the same kinetics pattern of TNF-a production with a peak at 18 hours, then gradually decrease and disappear at 96 hours. Cytolitic effect on the endothelial cell culture started at day 2 and day 3, then became totally lysed at day 5 and day 7 after infection with DEN-2 and DEN-3, respectively.
Keywords: TNF-a - endothel-cell culture � umbilical vein - dengue
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