RAPID CONFIRMATION OF RABIES ANTIGEN IN FORMALIN-FIXED PARAFIN-EMBEDDED TISSUE BY IMMUNOPATHOLOGIC DIAGNOSIS
ABSTRACT: Skunks experimentally infected with street virus rabies intramuscularly were used to demonstrate rabies viral antigen in paraffin-embedded tissue by immunopatholoa diagnosis approach, the avidin biotin complex (ABC) method. The use of ABC method provides a simple and sensitive method to lo...
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[Yogyakarta] : Universitas Gadjah Mada
1996
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| Summary: | ABSTRACT:
Skunks experimentally infected with street virus rabies intramuscularly were used to demonstrate rabies viral antigen in paraffin-embedded tissue by immunopatholoa diagnosis approach, the avidin biotin complex (ABC) method. The use of ABC method provides a simple and sensitive method to localize rabies virus antigen in formalinfixed tissues. The results of the present study also demonstrated that a procedures using formalin-fixed parafin-embeded tissue and stained immunopathologically by the ABC method was an excellent method for both preservation of morfological details of cells/tissues and demonstration of rabies virus antigen. |
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