Immunohistochemical detection of nerve growth factor and its receptors in the rat periodontal ligament during tooth movement
Objectives Nerve growth factor (NGF) and its receptors, p75 and tyrosine receptor kinase A (Trk A), have been shown to increase following trauma. The aims of this study were to examine changes in the detection of NGF and its receptors during orthodontic tooth movement in the rat, and the effects of...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Pergamon
2009
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/22589/1/Dr_akbar.pdf |
| _version_ | 1825646438497910784 |
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| author | Hussin, Akbar Sham Sampson, Wayne John Dreyer, Craig William Pierce, Angela Mary Ferguson, Ian Andrew |
| author_facet | Hussin, Akbar Sham Sampson, Wayne John Dreyer, Craig William Pierce, Angela Mary Ferguson, Ian Andrew |
| author_sort | Hussin, Akbar Sham |
| collection | IIUM |
| description | Objectives
Nerve growth factor (NGF) and its receptors, p75 and tyrosine receptor kinase A (Trk A), have been shown to increase following trauma. The aims of this study were to examine changes in the detection of NGF and its receptors during orthodontic tooth movement in the rat, and the effects of anti-NGF on these changes.
Design
Orthodontic separators were placed between the right maxillary first and second molars of Sprague–Dawley rats which were equally divided into two groups. Animals from the second group were injected with anti-NGF. The left sides served as controls, and animals were sacrificed at 0, 3, 7 and 14 days.
Results
Results of immunohistochemical localisation for p75, Trk A, calcitonin gene-related peptide (CGRP) and NGF showed staining intensity increased at day 3, with a peak at day 7 and decreasing intensity at day 14. Anti-NGF injected animals showed reduced staining at all observation periods.
Conclusion
Data suggest that orthodontic injury induces NGF production, leading to sprouting and invasion by CGRP-positive nerve fibers and that injection of anti-NGF reduces NGF tissue levels and prevents innervation by CGRP-positive fibers.
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| first_indexed | 2024-03-05T23:02:34Z |
| format | Article |
| id | oai:generic.eprints.org:22589 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-13T18:48:01Z |
| publishDate | 2009 |
| publisher | Pergamon |
| record_format | dspace |
| spelling | oai:generic.eprints.org:225892012-03-15T08:55:09Z http://irep.iium.edu.my/22589/ Immunohistochemical detection of nerve growth factor and its receptors in the rat periodontal ligament during tooth movement Hussin, Akbar Sham Sampson, Wayne John Dreyer, Craig William Pierce, Angela Mary Ferguson, Ian Andrew RK Dentistry Objectives Nerve growth factor (NGF) and its receptors, p75 and tyrosine receptor kinase A (Trk A), have been shown to increase following trauma. The aims of this study were to examine changes in the detection of NGF and its receptors during orthodontic tooth movement in the rat, and the effects of anti-NGF on these changes. Design Orthodontic separators were placed between the right maxillary first and second molars of Sprague–Dawley rats which were equally divided into two groups. Animals from the second group were injected with anti-NGF. The left sides served as controls, and animals were sacrificed at 0, 3, 7 and 14 days. Results Results of immunohistochemical localisation for p75, Trk A, calcitonin gene-related peptide (CGRP) and NGF showed staining intensity increased at day 3, with a peak at day 7 and decreasing intensity at day 14. Anti-NGF injected animals showed reduced staining at all observation periods. Conclusion Data suggest that orthodontic injury induces NGF production, leading to sprouting and invasion by CGRP-positive nerve fibers and that injection of anti-NGF reduces NGF tissue levels and prevents innervation by CGRP-positive fibers. Pergamon 2009-09 Article PeerReviewed application/pdf en http://irep.iium.edu.my/22589/1/Dr_akbar.pdf Hussin, Akbar Sham and Sampson, Wayne John and Dreyer, Craig William and Pierce, Angela Mary and Ferguson, Ian Andrew (2009) Immunohistochemical detection of nerve growth factor and its receptors in the rat periodontal ligament during tooth movement. Archives of Oral Biology, 54 (9). pp. 871-878. ISSN 0003-9969 http://www.elsevier.com/wps/find/journaldescription.cws_home/203/description#description |
| spellingShingle | RK Dentistry Hussin, Akbar Sham Sampson, Wayne John Dreyer, Craig William Pierce, Angela Mary Ferguson, Ian Andrew Immunohistochemical detection of nerve growth factor and its receptors in the rat periodontal ligament during tooth movement |
| title | Immunohistochemical detection of nerve growth factor and its receptors in the rat periodontal ligament during tooth movement |
| title_full | Immunohistochemical detection of nerve growth factor and its receptors in the rat periodontal ligament during tooth movement |
| title_fullStr | Immunohistochemical detection of nerve growth factor and its receptors in the rat periodontal ligament during tooth movement |
| title_full_unstemmed | Immunohistochemical detection of nerve growth factor and its receptors in the rat periodontal ligament during tooth movement |
| title_short | Immunohistochemical detection of nerve growth factor and its receptors in the rat periodontal ligament during tooth movement |
| title_sort | immunohistochemical detection of nerve growth factor and its receptors in the rat periodontal ligament during tooth movement |
| topic | RK Dentistry |
| url | http://irep.iium.edu.my/22589/1/Dr_akbar.pdf |
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