Tes sitotoksisitas bahan kalsium hidroksida dengan menggunakan kultur sel fibroblast embbio ayam kampung (Gallus domesticus) in vitro=Evaluation of the Cytotaxicity Effects of Calcium Hidroxide on Gallus domesticus Embry

The purpose of the present investigation was to study the cytotoxicity effect of calcium hydroxide in fibroblast cell culture system. Thirty plates of monolayer culteres of an established embrionic fibroblast cell of Gallus domesticus were used in this medium was decanted Filter paper disc were slur...

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主要作者: Perpustakaan UGM, i-lib
格式: 文件
出版: [Yogyakarta] : Universitas Gadjah Mada 1988
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author Perpustakaan UGM, i-lib
author_facet Perpustakaan UGM, i-lib
author_sort Perpustakaan UGM, i-lib
collection UGM
description The purpose of the present investigation was to study the cytotoxicity effect of calcium hydroxide in fibroblast cell culture system. Thirty plates of monolayer culteres of an established embrionic fibroblast cell of Gallus domesticus were used in this medium was decanted Filter paper disc were sluraled with calcium hydroxide in various concentration (1 persen, 3 persen, 6 persen, and 7 persen) and placed on the agar overlay and 1 filler paper disc was saturated- with steril aquadest as a control. The plates were returned to the incubator for 24 hours (15 plates) and 48 hours (15 plates). The agar was decanted and the monolayer was fixed in 10 persen formalin and stained with methyleen blue and the diameters of the zone of cell death were recorded. All four of the calcium hydroxide in various concentration produced cell death when filler paper disc were placed on the agar overlay. There were no zone of cell death around any of the control disc. This study indicates that there are significance differences between the four experimental groups and there is a significance positive corellation between the calcium hydroxide concentration and zone of cell death. After 48 hours the cytotoxicity effect of calcium hydroxide was decreased. Keywords: fibroblast - cytotoxicity - calcium hydroxide
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publisher [Yogyakarta] : Universitas Gadjah Mada
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spelling oai:generic.eprints.org:228212014-06-18T00:42:37Z https://repository.ugm.ac.id/22821/ Tes sitotoksisitas bahan kalsium hidroksida dengan menggunakan kultur sel fibroblast embbio ayam kampung (Gallus domesticus) in vitro=Evaluation of the Cytotaxicity Effects of Calcium Hidroxide on Gallus domesticus Embry Perpustakaan UGM, i-lib Jurnal i-lib UGM The purpose of the present investigation was to study the cytotoxicity effect of calcium hydroxide in fibroblast cell culture system. Thirty plates of monolayer culteres of an established embrionic fibroblast cell of Gallus domesticus were used in this medium was decanted Filter paper disc were sluraled with calcium hydroxide in various concentration (1 persen, 3 persen, 6 persen, and 7 persen) and placed on the agar overlay and 1 filler paper disc was saturated- with steril aquadest as a control. The plates were returned to the incubator for 24 hours (15 plates) and 48 hours (15 plates). The agar was decanted and the monolayer was fixed in 10 persen formalin and stained with methyleen blue and the diameters of the zone of cell death were recorded. All four of the calcium hydroxide in various concentration produced cell death when filler paper disc were placed on the agar overlay. There were no zone of cell death around any of the control disc. This study indicates that there are significance differences between the four experimental groups and there is a significance positive corellation between the calcium hydroxide concentration and zone of cell death. After 48 hours the cytotoxicity effect of calcium hydroxide was decreased. Keywords: fibroblast - cytotoxicity - calcium hydroxide [Yogyakarta] : Universitas Gadjah Mada 1988 Article NonPeerReviewed Perpustakaan UGM, i-lib (1988) Tes sitotoksisitas bahan kalsium hidroksida dengan menggunakan kultur sel fibroblast embbio ayam kampung (Gallus domesticus) in vitro=Evaluation of the Cytotaxicity Effects of Calcium Hidroxide on Gallus domesticus Embry. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=5745
spellingShingle Jurnal i-lib UGM
Perpustakaan UGM, i-lib
Tes sitotoksisitas bahan kalsium hidroksida dengan menggunakan kultur sel fibroblast embbio ayam kampung (Gallus domesticus) in vitro=Evaluation of the Cytotaxicity Effects of Calcium Hidroxide on Gallus domesticus Embry
title Tes sitotoksisitas bahan kalsium hidroksida dengan menggunakan kultur sel fibroblast embbio ayam kampung (Gallus domesticus) in vitro=Evaluation of the Cytotaxicity Effects of Calcium Hidroxide on Gallus domesticus Embry
title_full Tes sitotoksisitas bahan kalsium hidroksida dengan menggunakan kultur sel fibroblast embbio ayam kampung (Gallus domesticus) in vitro=Evaluation of the Cytotaxicity Effects of Calcium Hidroxide on Gallus domesticus Embry
title_fullStr Tes sitotoksisitas bahan kalsium hidroksida dengan menggunakan kultur sel fibroblast embbio ayam kampung (Gallus domesticus) in vitro=Evaluation of the Cytotaxicity Effects of Calcium Hidroxide on Gallus domesticus Embry
title_full_unstemmed Tes sitotoksisitas bahan kalsium hidroksida dengan menggunakan kultur sel fibroblast embbio ayam kampung (Gallus domesticus) in vitro=Evaluation of the Cytotaxicity Effects of Calcium Hidroxide on Gallus domesticus Embry
title_short Tes sitotoksisitas bahan kalsium hidroksida dengan menggunakan kultur sel fibroblast embbio ayam kampung (Gallus domesticus) in vitro=Evaluation of the Cytotaxicity Effects of Calcium Hidroxide on Gallus domesticus Embry
title_sort tes sitotoksisitas bahan kalsium hidroksida dengan menggunakan kultur sel fibroblast embbio ayam kampung gallus domesticus in vitro evaluation of the cytotaxicity effects of calcium hidroxide on gallus domesticus embry
topic Jurnal i-lib UGM
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