| Summary: | ABSTRACT
Research on biodegradation of Acacia mangium Wild. leaf litter by soil fungi was carried out. The aims of the research were to study the pattern of colonies of fungi grew on Acacia leaf litter and their abilities on degradation of litter.
The study was commenced by the observation on fungal colonies grow on forest floor at shaded and unshaded places at Wanagama I with, light intensity 10-30 lux and 35-60 lux, respectively. The types of fungi grow on those litters were isolated with selective media. The fungi were cultivated on media containing xylan, cellulose (CMC), or lignin. The fungal growth was monitored by measuring mycelium dry weight. The enzyme activity was elucidated using cell free extract against xylan, CMC, and lignin. The fungal ability on degradation of leaf litter was observed. The enzyme profil was visualized using SDS-polyacrylamide gel electrophoresis.
The result showed that fungal colonies more spreadly and densely at shaded places than unshaded places with radius 20-50 cm and 12-25 cm, respectively. Three isolat were identified as Curvularia sp (T2), Alternaria sp (TT2), and Trichoderma sp (T4) had the higest lignolitic ability. These isolates were able to grow on medium containing xylan, CMC, or lignin as sole carbon sources. Trichoderma sp grew better with spesific growth (h-1): on xylan 0.128
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