The phytochemical content and antimicrobial activities of Malaysian Calophyllum canum (stem bark)
Recently there was huge increase in using of ‘herbal products’. These can be defined as plants, parts of plants or extracts from plants that are used for curing disease. However, Calophyllum species is a tropical plant and it has been used in traditional medicine, the limitation in safety and effec...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Pharmacy, University of Karachi
2012
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/24948/2/Pak_J_Pharm_Sci.pdf |
| _version_ | 1825646694510886912 |
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| author | Alkhamaiseh, Suhaib Ibrahim Bakhtiar, M. Taher Ahmad, Farediah Qaralleh, Haitham Althunibat, Osama Yousef Darnis, Deny Susanti Solachuddin Jauhari, Arief Ichwan |
| author_facet | Alkhamaiseh, Suhaib Ibrahim Bakhtiar, M. Taher Ahmad, Farediah Qaralleh, Haitham Althunibat, Osama Yousef Darnis, Deny Susanti Solachuddin Jauhari, Arief Ichwan |
| author_sort | Alkhamaiseh, Suhaib Ibrahim |
| collection | IIUM |
| description | Recently there was huge increase in using of ‘herbal products’. These can be defined as plants, parts of plants
or extracts from plants that are used for curing disease. However, Calophyllum species is a tropical plant and it has been used in traditional medicine, the limitation in safety and effectiveness information could lead to serious health problems.
Providing information for communities by evaluating the phytochemical contents, antioxidant, antimicrobial and
cytotoxic activities will improve the therapeutic values. Three main Calophyllum canum fractions (none – high polar)
were tested to find out the phenolic, flavonoid, flavonol content, DPPH radical scavenging, reducing power and chelating iron ions. Also were tested against Bacillus cereus, Staphylococcus aureus, Escherichia coli, Psedomonas aeruginosa, Candida albicans, and Cryptococcus neoformans. In addition, cytotoxic activity was assayed against lung cancer A549 cell line. The methanol fraction showed no bioactivity but achieved the highest amount of phenolic, flavonol and flavonoid contents, also it showed a significant result as antioxidant, reducing power and chelating agent. The n-hexane fraction achieved the minimum inhibitory concentration (MIC) value 12.5 μg. mL-1 against B. cereus while the MIC value for DCM fraction was 25 μg. mL-1. The DCM fraction was more active against S. aureus where the result was 50 μg. mL-1 while the n-hexane fraction was 100 μg. mL-1. The three main fractions have shown no activity against gram negative bacterial and fungal. The n-hexane and DCM fractions have shown cytotoxicity against lung cancer cell line;
the 50 % inhibition concentration (IC50) was 22 ± 2.64 and 32 ± 3.78 μg. mL-1 respectively. The results were statistically significant (P < 0.05). Among the results, C. canum fractions proved to be effective against gram positive bacterial and anti-proliferation activity. Also it showed antioxidant activity as well. The results provided beneficial information for communities as well as can help to search for alternative drugs, and will contribute to establish safe and effective use of phytomedicines in the treatment of diseases. |
| first_indexed | 2024-03-05T23:06:24Z |
| format | Article |
| id | oai:generic.eprints.org:24948 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-13T18:55:31Z |
| publishDate | 2012 |
| publisher | Faculty of Pharmacy, University of Karachi |
| record_format | dspace |
| spelling | oai:generic.eprints.org:249482014-05-22T01:25:19Z http://irep.iium.edu.my/24948/ The phytochemical content and antimicrobial activities of Malaysian Calophyllum canum (stem bark) Alkhamaiseh, Suhaib Ibrahim Bakhtiar, M. Taher Ahmad, Farediah Qaralleh, Haitham Althunibat, Osama Yousef Darnis, Deny Susanti Solachuddin Jauhari, Arief Ichwan RS Pharmacy and materia medica Recently there was huge increase in using of ‘herbal products’. These can be defined as plants, parts of plants or extracts from plants that are used for curing disease. However, Calophyllum species is a tropical plant and it has been used in traditional medicine, the limitation in safety and effectiveness information could lead to serious health problems. Providing information for communities by evaluating the phytochemical contents, antioxidant, antimicrobial and cytotoxic activities will improve the therapeutic values. Three main Calophyllum canum fractions (none – high polar) were tested to find out the phenolic, flavonoid, flavonol content, DPPH radical scavenging, reducing power and chelating iron ions. Also were tested against Bacillus cereus, Staphylococcus aureus, Escherichia coli, Psedomonas aeruginosa, Candida albicans, and Cryptococcus neoformans. In addition, cytotoxic activity was assayed against lung cancer A549 cell line. The methanol fraction showed no bioactivity but achieved the highest amount of phenolic, flavonol and flavonoid contents, also it showed a significant result as antioxidant, reducing power and chelating agent. The n-hexane fraction achieved the minimum inhibitory concentration (MIC) value 12.5 μg. mL-1 against B. cereus while the MIC value for DCM fraction was 25 μg. mL-1. The DCM fraction was more active against S. aureus where the result was 50 μg. mL-1 while the n-hexane fraction was 100 μg. mL-1. The three main fractions have shown no activity against gram negative bacterial and fungal. The n-hexane and DCM fractions have shown cytotoxicity against lung cancer cell line; the 50 % inhibition concentration (IC50) was 22 ± 2.64 and 32 ± 3.78 μg. mL-1 respectively. The results were statistically significant (P < 0.05). Among the results, C. canum fractions proved to be effective against gram positive bacterial and anti-proliferation activity. Also it showed antioxidant activity as well. The results provided beneficial information for communities as well as can help to search for alternative drugs, and will contribute to establish safe and effective use of phytomedicines in the treatment of diseases. Faculty of Pharmacy, University of Karachi 2012-07 Article PeerReviewed application/pdf en http://irep.iium.edu.my/24948/2/Pak_J_Pharm_Sci.pdf Alkhamaiseh, Suhaib Ibrahim and Bakhtiar, M. Taher and Ahmad, Farediah and Qaralleh, Haitham and Althunibat, Osama Yousef and Darnis, Deny Susanti and Solachuddin Jauhari, Arief Ichwan (2012) The phytochemical content and antimicrobial activities of Malaysian Calophyllum canum (stem bark). Pakistan Journal of Pharmaceutical Sciences, 25 (3). pp. 555-563. ISSN 11011-601X http://www.pjps.pk/current.htm |
| spellingShingle | RS Pharmacy and materia medica Alkhamaiseh, Suhaib Ibrahim Bakhtiar, M. Taher Ahmad, Farediah Qaralleh, Haitham Althunibat, Osama Yousef Darnis, Deny Susanti Solachuddin Jauhari, Arief Ichwan The phytochemical content and antimicrobial activities of Malaysian Calophyllum canum (stem bark) |
| title | The phytochemical content and antimicrobial activities of Malaysian Calophyllum canum (stem bark) |
| title_full | The phytochemical content and antimicrobial activities of Malaysian Calophyllum canum (stem bark) |
| title_fullStr | The phytochemical content and antimicrobial activities of Malaysian Calophyllum canum (stem bark) |
| title_full_unstemmed | The phytochemical content and antimicrobial activities of Malaysian Calophyllum canum (stem bark) |
| title_short | The phytochemical content and antimicrobial activities of Malaysian Calophyllum canum (stem bark) |
| title_sort | phytochemical content and antimicrobial activities of malaysian calophyllum canum stem bark |
| topic | RS Pharmacy and materia medica |
| url | http://irep.iium.edu.my/24948/2/Pak_J_Pharm_Sci.pdf |
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