Pengembangan Diagnostik Spesies Kriptik Pada Nyamuk (Diptera: Culicidae) Dengan Metoda Random Amplified Polymorphic DNA (RAPD)

Abstract Budi Mulyaningsih-Random amplified polymorphic DNA (RaPD) markers readily distinguish crytic mosquito species(diptera:culicidae) cryptic species complexes are groups of closely related species that are difficult of impossble to be didtingueshed by morphological trait.These complexes are kno...

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Autor principal: Perpustakaan UGM, i-lib
Formato: Artigo
Publicado em: [Yogyakarta] : Universitas Gadjah Mada 2002
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author Perpustakaan UGM, i-lib
author_facet Perpustakaan UGM, i-lib
author_sort Perpustakaan UGM, i-lib
collection UGM
description Abstract Budi Mulyaningsih-Random amplified polymorphic DNA (RaPD) markers readily distinguish crytic mosquito species(diptera:culicidae) cryptic species complexes are groups of closely related species that are difficult of impossble to be didtingueshed by morphological trait.These complexes are knoe from a wide veriety of arthropods and are common among the well-studied,medically-important insects.Various methods have been developedfor identifying the species of individual specimen from these complexes.Recently, the use of polymerase chain reaction(PCR) to amplify DNA has become widespread.By the development of PCR method that employs a single, 10 base long primer of arbitrary DNA sequence, random amplified polymorphic DNA(RAPD) was used to differentiate closely related species.The Problem discussed is whether the RAPD.PCR is effective to differentiate cryptic mosquito species.This method has been applied in many studies to answer the problem.It proved to be a quick effective mean of finding genetic markers to separate populations of morphologically indistinguishable mosquito. Key words:cryptic species-mosquito-polymorphic DNA - RARP-PCR
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spelling oai:generic.eprints.org:250722014-06-18T00:32:00Z https://repository.ugm.ac.id/25072/ Pengembangan Diagnostik Spesies Kriptik Pada Nyamuk (Diptera: Culicidae) Dengan Metoda Random Amplified Polymorphic DNA (RAPD) Perpustakaan UGM, i-lib Jurnal i-lib UGM Abstract Budi Mulyaningsih-Random amplified polymorphic DNA (RaPD) markers readily distinguish crytic mosquito species(diptera:culicidae) cryptic species complexes are groups of closely related species that are difficult of impossble to be didtingueshed by morphological trait.These complexes are knoe from a wide veriety of arthropods and are common among the well-studied,medically-important insects.Various methods have been developedfor identifying the species of individual specimen from these complexes.Recently, the use of polymerase chain reaction(PCR) to amplify DNA has become widespread.By the development of PCR method that employs a single, 10 base long primer of arbitrary DNA sequence, random amplified polymorphic DNA(RAPD) was used to differentiate closely related species.The Problem discussed is whether the RAPD.PCR is effective to differentiate cryptic mosquito species.This method has been applied in many studies to answer the problem.It proved to be a quick effective mean of finding genetic markers to separate populations of morphologically indistinguishable mosquito. Key words:cryptic species-mosquito-polymorphic DNA - RARP-PCR [Yogyakarta] : Universitas Gadjah Mada 2002 Article NonPeerReviewed Perpustakaan UGM, i-lib (2002) Pengembangan Diagnostik Spesies Kriptik Pada Nyamuk (Diptera: Culicidae) Dengan Metoda Random Amplified Polymorphic DNA (RAPD). Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=8054
spellingShingle Jurnal i-lib UGM
Perpustakaan UGM, i-lib
Pengembangan Diagnostik Spesies Kriptik Pada Nyamuk (Diptera: Culicidae) Dengan Metoda Random Amplified Polymorphic DNA (RAPD)
title Pengembangan Diagnostik Spesies Kriptik Pada Nyamuk (Diptera: Culicidae) Dengan Metoda Random Amplified Polymorphic DNA (RAPD)
title_full Pengembangan Diagnostik Spesies Kriptik Pada Nyamuk (Diptera: Culicidae) Dengan Metoda Random Amplified Polymorphic DNA (RAPD)
title_fullStr Pengembangan Diagnostik Spesies Kriptik Pada Nyamuk (Diptera: Culicidae) Dengan Metoda Random Amplified Polymorphic DNA (RAPD)
title_full_unstemmed Pengembangan Diagnostik Spesies Kriptik Pada Nyamuk (Diptera: Culicidae) Dengan Metoda Random Amplified Polymorphic DNA (RAPD)
title_short Pengembangan Diagnostik Spesies Kriptik Pada Nyamuk (Diptera: Culicidae) Dengan Metoda Random Amplified Polymorphic DNA (RAPD)
title_sort pengembangan diagnostik spesies kriptik pada nyamuk diptera culicidae dengan metoda random amplified polymorphic dna rapd
topic Jurnal i-lib UGM
work_keys_str_mv AT perpustakaanugmilib pengembangandiagnostikspesieskriptikpadanyamukdipteraculicidaedenganmetodarandomamplifiedpolymorphicdnarapd