Uji Diagnostik Serologi Terhadap antigenemia Sebagai Penanda Diagnostik Untuk Infeksi Cytomegalovirus Pada Bayi Dan Anak
Agnes Yunie, Sunartini, Madarina Julia - Serologic test vs antigenemia assay as diagnostic marker for cytomegalovirus infection in children. Objective: To determine the level IgG and IgM anti-CMV in children clinically suspected to be infected by Cytomegalovirus (CMV) and to determine the diagnostic...
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[Yogyakarta] : Universitas Gadjah Mada
2002
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| _version_ | 1826032586959355904 |
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| author | Perpustakaan UGM, i-lib |
| author_facet | Perpustakaan UGM, i-lib |
| author_sort | Perpustakaan UGM, i-lib |
| collection | UGM |
| description | Agnes Yunie, Sunartini, Madarina Julia - Serologic test vs antigenemia assay as diagnostic marker for cytomegalovirus infection in children.
Objective: To determine the level IgG and IgM anti-CMV in children clinically suspected to be infected by Cytomegalovirus (CMV) and to determine the diagnostic value of IgG dan IgM anti-CMV as diagnostic tools for active CMV infection with antigenemia assay as the gold standard.
Material and methods: Diagnostic test, observational cross sectional study. All children suspected to have CMV infection who were hospitalized or who visited neurology outpatient clinic in Sardjito Hospital in March 1" - September 11" 2002 were enrolled in this study. Serologic test for IgG and IgM anti-CM- and antigenemia assay were conducted to confirm the diagnosis of active CMV infection.
Results: Nine of 82 specimens (12%) were confirmed to have active CMV. The cut off point of IgG was 265.75 AU/ml, with the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of 67% (95% CI: 31-91%), 70% (95% CI: 58-80%), 21% (95% CI: 9-42%), 94% (95% CI: 84-99%), respectively, and likelihood ratio (LR) 4.5. The cut off point of IgM was 0.445, with the sensitivity, specificity, PPV and NPV of 56% (95% CI: 23-85%), 67% (95% CI: 55-77%), 17% (95% CI: 7-37%) and 93% (95% CI: 81-98%), respectively and LR 1.7. There was no significant relation between the level of IgG or IgM above cut off point and antigenemia (p=0.06 and p =0.27). Level of IgG combined with IgM had a significant relation with antigenemia (p 0.04), with the sensitivity 75% (95% CI: 22-99%), specificity 81% (95% CI: 66-91%), PPV 27% (95% CI: 7-61%), NPV 97% (95% CI: 84100%) respectively. LR was 5.3. Most of the children (40 patients) had psychomotor disorder (60%). There was significant relation between trombocytopenia and antigenemia (p =0.04).
Conclusions: An increased level of IgM combined with IgG above cut off point is a significant diagnostic tool for active CMV infection. Psychomotor disorder is the clinically sign mostly found in active CMV infection. Thrombocytopenia can be used to detect active CMV infection.
Key words: active CMV infection - antigenemia - IgM & IgG anti-CMV - IgM anti-CMV - diagnostic test |
| first_indexed | 2024-03-05T23:06:47Z |
| format | Article |
| id | oai:generic.eprints.org:25145 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-05T23:06:47Z |
| publishDate | 2002 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:251452014-06-18T00:32:20Z https://repository.ugm.ac.id/25145/ Uji Diagnostik Serologi Terhadap antigenemia Sebagai Penanda Diagnostik Untuk Infeksi Cytomegalovirus Pada Bayi Dan Anak Perpustakaan UGM, i-lib Jurnal i-lib UGM Agnes Yunie, Sunartini, Madarina Julia - Serologic test vs antigenemia assay as diagnostic marker for cytomegalovirus infection in children. Objective: To determine the level IgG and IgM anti-CMV in children clinically suspected to be infected by Cytomegalovirus (CMV) and to determine the diagnostic value of IgG dan IgM anti-CMV as diagnostic tools for active CMV infection with antigenemia assay as the gold standard. Material and methods: Diagnostic test, observational cross sectional study. All children suspected to have CMV infection who were hospitalized or who visited neurology outpatient clinic in Sardjito Hospital in March 1" - September 11" 2002 were enrolled in this study. Serologic test for IgG and IgM anti-CM- and antigenemia assay were conducted to confirm the diagnosis of active CMV infection. Results: Nine of 82 specimens (12%) were confirmed to have active CMV. The cut off point of IgG was 265.75 AU/ml, with the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of 67% (95% CI: 31-91%), 70% (95% CI: 58-80%), 21% (95% CI: 9-42%), 94% (95% CI: 84-99%), respectively, and likelihood ratio (LR) 4.5. The cut off point of IgM was 0.445, with the sensitivity, specificity, PPV and NPV of 56% (95% CI: 23-85%), 67% (95% CI: 55-77%), 17% (95% CI: 7-37%) and 93% (95% CI: 81-98%), respectively and LR 1.7. There was no significant relation between the level of IgG or IgM above cut off point and antigenemia (p=0.06 and p =0.27). Level of IgG combined with IgM had a significant relation with antigenemia (p 0.04), with the sensitivity 75% (95% CI: 22-99%), specificity 81% (95% CI: 66-91%), PPV 27% (95% CI: 7-61%), NPV 97% (95% CI: 84100%) respectively. LR was 5.3. Most of the children (40 patients) had psychomotor disorder (60%). There was significant relation between trombocytopenia and antigenemia (p =0.04). Conclusions: An increased level of IgM combined with IgG above cut off point is a significant diagnostic tool for active CMV infection. Psychomotor disorder is the clinically sign mostly found in active CMV infection. Thrombocytopenia can be used to detect active CMV infection. Key words: active CMV infection - antigenemia - IgM & IgG anti-CMV - IgM anti-CMV - diagnostic test [Yogyakarta] : Universitas Gadjah Mada 2002 Article NonPeerReviewed Perpustakaan UGM, i-lib (2002) Uji Diagnostik Serologi Terhadap antigenemia Sebagai Penanda Diagnostik Untuk Infeksi Cytomegalovirus Pada Bayi Dan Anak. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=8130 |
| spellingShingle | Jurnal i-lib UGM Perpustakaan UGM, i-lib Uji Diagnostik Serologi Terhadap antigenemia Sebagai Penanda Diagnostik Untuk Infeksi Cytomegalovirus Pada Bayi Dan Anak |
| title | Uji Diagnostik Serologi Terhadap antigenemia Sebagai Penanda Diagnostik Untuk Infeksi Cytomegalovirus Pada Bayi Dan Anak |
| title_full | Uji Diagnostik Serologi Terhadap antigenemia Sebagai Penanda Diagnostik Untuk Infeksi Cytomegalovirus Pada Bayi Dan Anak |
| title_fullStr | Uji Diagnostik Serologi Terhadap antigenemia Sebagai Penanda Diagnostik Untuk Infeksi Cytomegalovirus Pada Bayi Dan Anak |
| title_full_unstemmed | Uji Diagnostik Serologi Terhadap antigenemia Sebagai Penanda Diagnostik Untuk Infeksi Cytomegalovirus Pada Bayi Dan Anak |
| title_short | Uji Diagnostik Serologi Terhadap antigenemia Sebagai Penanda Diagnostik Untuk Infeksi Cytomegalovirus Pada Bayi Dan Anak |
| title_sort | uji diagnostik serologi terhadap antigenemia sebagai penanda diagnostik untuk infeksi cytomegalovirus pada bayi dan anak |
| topic | Jurnal i-lib UGM |
| work_keys_str_mv | AT perpustakaanugmilib ujidiagnostikserologiterhadapantigenemiasebagaipenandadiagnostikuntukinfeksicytomegaloviruspadabayidananak |