| Summary: | Tujuan penelitian ini adalah mempelajari profil anti-inhibin hasil induksi inhibin 32 kDa dari sel granulosa folikel ovarium kambing pada kelinci. Penelitian ini dilakukan dilakukan dalam 2 tahap yaitu 1) isolasi dan karakterisasi inhibin dari sel granulosa folikel ovarium kambing dan 2) karakterisasi anti-inhibin sebagai respon imun akibat induksi oleh inhibin. Karakterisasi inhibin dilakukan menggunakan teknik sodium dodecyl sulphonat polyacrylamide gel electrophoresis (SDS-PAGE) dan anti-inhibin dikarakterisasi menggunakan teknik blotting (dot blot dan Western blot) dan enzyme-linked immunosorbent assay (ELISA). Imunisasi 200 p.1 inhibin dilakukan dengan penambahan ill 200 Complete Freund's Adjuvant (CFA) dan 200111 Incomplete Freund's Adjuvant (IFA) masing-masing untuk imunisasi primer dan booster. Serum dikoleksi setiap minggu selama 10 kali setelah booster I. Hasil SDS-PAGE menunjukkan bahwa inhibin sel granulosa folikel ovarium kambing mempunyai BM 32 kDa. Imunisasi primer yang diikuti 2 kali booster menginduksi respon imun humoral yang ditandai dengan biosintesis anti-inhibin. Titer optimum dicapai pada pengambilan darah ke-8 (65 hari pasca imunisasi primer).
Kata kanci: inhibin, anti-inhibin, imunisasi, booster, titer
The objective of this research was evaluate the profil of anti-inhibin induced by inhibin 32 kDa isolated from goat granulosa on rabbit (Oryctolagus cunniculus). Two stages research were conducted : stage 1, isolation and characterization inhibin from goat granulosa and stage II, characterization of anti-inhibin. Characterization of inhibin was done by technique sodium dodecyl sulphonat polyacrylamide gel electrophoresis (SDS-PAGE) and anti-inhibin characterized by technique of dot blot, Western blot and enzyme-linked immunosorbent assay (ELISA). The rabbit were immunized by 200 1.11 inhibin with 200 p.1 Complete Freund's Adjuvant (CFA) and 200 Al Incomplete Freund's Adjuvant (IFA) for primary immunization and booster immunization, respectively. Serum were collected 10 times. SDS-PAGE revealed that inhibin have molecular weights of 32 kDa. Primary immunization and following by 2 times booster immunization induced biosynthesis of antibodies to inhibin and have optimum titre on 8th bleed (65 days after first immunization ).
Key words: inhibin, anti-inhibin, immunization, booster, titre
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