Isolation of Stable Mutants of Candida guilliermondii Producing One Type of ADH
ABSTRAK Mutants of Candida guilliermondii partially deficient in alcohol dehydrogenase (ADH) were isolated using allyl alcohol as a selective agent. On glucose medium containing ally] alcohol produced mutants deficient in ADH1, while mutants free of ADH2 were isolated on ethanol medium containing al...
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[Yogyakarta] : Universitas Gadjah Mada
1994
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| _version_ | 1797019341229654016 |
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| author | Perpustakaan UGM, i-lib |
| author_facet | Perpustakaan UGM, i-lib |
| author_sort | Perpustakaan UGM, i-lib |
| collection | UGM |
| description | ABSTRAK
Mutants of Candida guilliermondii partially deficient in alcohol dehydrogenase (ADH) were isolated using allyl alcohol as a selective agent. On glucose medium containing ally] alcohol produced mutants deficient in ADH1, while mutants free of ADH2 were isolated on ethanol medium containing allyl alcohol. An addition of 1% of yeast extract to the isolation medium resulted in a stability of the mutants against a high concentration of allyl alcohol. On this medium the cells resistant up to 80 mM of allyl alcohol compared to those with a resistance of 10 mM allyl alcohol on basal salt medium without addition of yeast extract. Furthermore, this resistance to ally/ alcohol seems to be related to a stability of the mutants to produce only one type of ADH for a long incubation period (more than 2 years). Cells which were resistant only up to 10 mM of ally] alcohol started to produce two kind of ADHs after six months of incubation. However, this resistance to a high allyl alcohol resulted in a decrease of specific activity of ADHs. Mutant ADH1s had activities only 23 � 50%, where as mutant ADH2s had only 1 � 3% of the parent strain activity.
Keywords: Candida guilliermondii , alcohol dehydrogenase |
| first_indexed | 2024-03-05T23:07:52Z |
| format | Article |
| id | oai:generic.eprints.org:25788 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T18:58:05Z |
| publishDate | 1994 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:257882014-06-18T00:39:39Z https://repository.ugm.ac.id/25788/ Isolation of Stable Mutants of Candida guilliermondii Producing One Type of ADH Perpustakaan UGM, i-lib Jurnal i-lib UGM ABSTRAK Mutants of Candida guilliermondii partially deficient in alcohol dehydrogenase (ADH) were isolated using allyl alcohol as a selective agent. On glucose medium containing ally] alcohol produced mutants deficient in ADH1, while mutants free of ADH2 were isolated on ethanol medium containing allyl alcohol. An addition of 1% of yeast extract to the isolation medium resulted in a stability of the mutants against a high concentration of allyl alcohol. On this medium the cells resistant up to 80 mM of allyl alcohol compared to those with a resistance of 10 mM allyl alcohol on basal salt medium without addition of yeast extract. Furthermore, this resistance to ally/ alcohol seems to be related to a stability of the mutants to produce only one type of ADH for a long incubation period (more than 2 years). Cells which were resistant only up to 10 mM of ally] alcohol started to produce two kind of ADHs after six months of incubation. However, this resistance to a high allyl alcohol resulted in a decrease of specific activity of ADHs. Mutant ADH1s had activities only 23 � 50%, where as mutant ADH2s had only 1 � 3% of the parent strain activity. Keywords: Candida guilliermondii , alcohol dehydrogenase [Yogyakarta] : Universitas Gadjah Mada 1994 Article NonPeerReviewed Perpustakaan UGM, i-lib (1994) Isolation of Stable Mutants of Candida guilliermondii Producing One Type of ADH. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=8788 |
| spellingShingle | Jurnal i-lib UGM Perpustakaan UGM, i-lib Isolation of Stable Mutants of Candida guilliermondii Producing One Type of ADH |
| title | Isolation of Stable Mutants of Candida guilliermondii Producing One Type of ADH |
| title_full | Isolation of Stable Mutants of Candida guilliermondii Producing One Type of ADH |
| title_fullStr | Isolation of Stable Mutants of Candida guilliermondii Producing One Type of ADH |
| title_full_unstemmed | Isolation of Stable Mutants of Candida guilliermondii Producing One Type of ADH |
| title_short | Isolation of Stable Mutants of Candida guilliermondii Producing One Type of ADH |
| title_sort | isolation of stable mutants of candida guilliermondii producing one type of adh |
| topic | Jurnal i-lib UGM |
| work_keys_str_mv | AT perpustakaanugmilib isolationofstablemutantsofcandidaguilliermondiiproducingonetypeofadh |