| Summary: | Abstract
The aim of this research was to detect and identify white spot syndrome virus (WSSV) infection in white shrimp (Penaus vannamei) in Java island by one and double steps poly-merase chain reaction (PCR) method, and histopatological examination. Sample of local and imported shrimp were collected from Situbondo (Asem Bagus, Arjasa, Pecaron, Besuki, Bungatan), Banyuwangi, Kendal (Kaliwungu), Banten, Yogyakarta, Sukabumi, Indramayu and Hawaii. The age of shrimp was 33 days, 35 days, 50 days, 60 days, 82 days, 85 days, 105 days and 180 days. Antenna, pleopod, gills and carapace were examined for histopatholocigal changes. DNA samples were extracted by PROMEGA method and amplified by one and double steps of PCR. The amplification result indicated that positive bands were found in samples collected from two locations of Besuki area and imported broodstock shrimp from Hawaii. The infected shrimps were detected at 35, 50 and 180 days of ages. Histopathological observation showed hypertrophied nuclei basophilic inclusion bodies on gills and pleopod of white shrimp from Besuki 1, on gills, from Besuki 2 and on pleopod, gills, hepatopancreas tissue of imported broodstock shrimp sample from Hawaii.
Key words: PCR, Penaeus vannamei, white spot syndrome virus (WSSV
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