| Summary: | ABSTRACT:
The protoplast fusion bctwccn Alucor pusillus T in, Mucor /walla FNC(*, Atricor Ocher FNCC and Mucor rouxil was studied to develop a breeding system for the microbial rennin producing !linear:Iles fungi. Protoplast from y01111V, gerniling of these fungi were obtariux1 by iismg enzymes Cocktail
front Helix ponuma and Cocktail II from Trichoderma harzianlin containing 13-ghicoronalase, sulfatasc, 1.3. _-glireariase and chillikase 'lite fusion of piotoplast of these organisms were stimulated by polyethylene glvcol(11EG) and Ca 2' 1011S atconcentration or 45 percents �tH)0 with NI
CaCl2 and incubated al room temperature for I's
minutes Vali:Won of elotting(lierridge
method) and 1ir leo ly tic activitiesOnscin
speetrophoininetrie method) of the gii.r.vtli of the
organisms lit the liamentation media containing lice
bran and minerals vixre iileastned. results
indicated that licterokaryon of /threw radius 10 with Mucor pustlius PAK I ' gave the highest value of milk-clotting activity:then:Rue it is considered as potential Mucor rennin producer
Key Words Mucor Rennin. Protoplast Fuston. Milk-Clotting, Iaroteolvitc Aetivisty
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