Analisis Komparatif Gen aroA Pasteurella Multocida Isolat Indonesia dengan PCR-SSCP
ABSTRACT Pasteurella multocida serotype B:2 is a primary pathogen bacterium that cause haemorrhagic septicaemia or primary pasteurellosis, the disease attacks the cattle, cows and buffaloes. The greatest economic losses are recorded in Asia, especially in Indonesia. The controlling endeavor that had...
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| Format: | Article |
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[Yogyakarta] : Universitas Gadjah Mada
2003
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| _version_ | 1797019639012655104 |
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| author | Perpustakaan UGM, i-lib |
| author_facet | Perpustakaan UGM, i-lib |
| author_sort | Perpustakaan UGM, i-lib |
| collection | UGM |
| description | ABSTRACT
Pasteurella multocida serotype B:2 is a primary pathogen bacterium that cause haemorrhagic septicaemia or primary pasteurellosis, the disease attacks the cattle, cows and buffaloes. The greatest economic losses are recorded in Asia, especially in Indonesia. The controlling endeavor that had ever been run was vaccination by giving inactive vaccine from B:2 P.multocida strain Katha from Myanmar in oil adjuvant form. The result was the vaccination did not protect 100% cattle in Indonesia.
Auxotrophic mutation in the capability of P. multocida serotype B:2 in synthesis aromatic amino acids is another attempt to generate live vaccine.
The amplified fragment was then analysed with RFLP using HpaII and producing two types of band sized 740 and 460 bp. SSCP analysis then conducted to observe if there was a sequence variant indicated by the mobility shift. DNA sequence variant in aroA was indicated by the mobility shift. The research resulted that PCR-SSCP analysis in aroA P. multocida B:2 did not demonstrate the occurrence of mobility shift so it indicated that there were not any differences among the isolated isolates/ strains.
Key words : pasteurellosis, Pasteurella multocida serotype B:2, aroA gene, PCR-RFLP and PCR-SSCP |
| first_indexed | 2024-03-05T23:10:31Z |
| format | Article |
| id | oai:generic.eprints.org:27188 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T19:02:44Z |
| publishDate | 2003 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:271882014-06-18T00:29:39Z https://repository.ugm.ac.id/27188/ Analisis Komparatif Gen aroA Pasteurella Multocida Isolat Indonesia dengan PCR-SSCP Perpustakaan UGM, i-lib Jurnal i-lib UGM ABSTRACT Pasteurella multocida serotype B:2 is a primary pathogen bacterium that cause haemorrhagic septicaemia or primary pasteurellosis, the disease attacks the cattle, cows and buffaloes. The greatest economic losses are recorded in Asia, especially in Indonesia. The controlling endeavor that had ever been run was vaccination by giving inactive vaccine from B:2 P.multocida strain Katha from Myanmar in oil adjuvant form. The result was the vaccination did not protect 100% cattle in Indonesia. Auxotrophic mutation in the capability of P. multocida serotype B:2 in synthesis aromatic amino acids is another attempt to generate live vaccine. The amplified fragment was then analysed with RFLP using HpaII and producing two types of band sized 740 and 460 bp. SSCP analysis then conducted to observe if there was a sequence variant indicated by the mobility shift. DNA sequence variant in aroA was indicated by the mobility shift. The research resulted that PCR-SSCP analysis in aroA P. multocida B:2 did not demonstrate the occurrence of mobility shift so it indicated that there were not any differences among the isolated isolates/ strains. Key words : pasteurellosis, Pasteurella multocida serotype B:2, aroA gene, PCR-RFLP and PCR-SSCP [Yogyakarta] : Universitas Gadjah Mada 2003 Article NonPeerReviewed Perpustakaan UGM, i-lib (2003) Analisis Komparatif Gen aroA Pasteurella Multocida Isolat Indonesia dengan PCR-SSCP. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=10240 |
| spellingShingle | Jurnal i-lib UGM Perpustakaan UGM, i-lib Analisis Komparatif Gen aroA Pasteurella Multocida Isolat Indonesia dengan PCR-SSCP |
| title | Analisis Komparatif Gen aroA Pasteurella Multocida Isolat Indonesia dengan PCR-SSCP |
| title_full | Analisis Komparatif Gen aroA Pasteurella Multocida Isolat Indonesia dengan PCR-SSCP |
| title_fullStr | Analisis Komparatif Gen aroA Pasteurella Multocida Isolat Indonesia dengan PCR-SSCP |
| title_full_unstemmed | Analisis Komparatif Gen aroA Pasteurella Multocida Isolat Indonesia dengan PCR-SSCP |
| title_short | Analisis Komparatif Gen aroA Pasteurella Multocida Isolat Indonesia dengan PCR-SSCP |
| title_sort | analisis komparatif gen aroa pasteurella multocida isolat indonesia dengan pcr sscp |
| topic | Jurnal i-lib UGM |
| work_keys_str_mv | AT perpustakaanugmilib analisiskomparatifgenaroapasteurellamultocidaisolatindonesiadenganpcrsscp |