In vitro fresh sperm preparation for maintaining sperm viability at Storage temperature of l4oC using tannin supplementation of lamtoro leaves

A research on in vitro fresh sperm preparation for maintaining sperm viability at storage temperature of l4 derajat C using tannin supplementation of lamtoro leaves (Leucaena leucocephala)had been carried out in both Physiology and Animal Reproduction Laboratory, Faculty of Animal Science, Universit...

Full description

Bibliographic Details
Main Authors: Mirajuddin, Mirajuddin, Kustono, Kustono, Ismaya, Ismaya, Budiyanto, Agung
Format: Article
Language:English
Published: Fakultas Peternakan Universitas Gadjah Mada 2010
Subjects:
Online Access:https://repository.ugm.ac.id/273136/1/1.%205%20th%20ISTAP%20International%20Seminar%20On%20Tropical%20Animal%20Production%20Proceedings%20Part%202.pdf
_version_ 1826049678470283264
author Mirajuddin, Mirajuddin
Kustono, Kustono
Ismaya, Ismaya
Budiyanto, Agung
author_facet Mirajuddin, Mirajuddin
Kustono, Kustono
Ismaya, Ismaya
Budiyanto, Agung
author_sort Mirajuddin, Mirajuddin
collection UGM
description A research on in vitro fresh sperm preparation for maintaining sperm viability at storage temperature of l4 derajat C using tannin supplementation of lamtoro leaves (Leucaena leucocephala)had been carried out in both Physiology and Animal Reproduction Laboratory, Faculty of Animal Science, Universitas Gadjah Mada for fresh sperm preparation and sperm quality evaluation, during March to July of 2009.The experiment was designed using the Complete Random Design consisted of 5 treatments and 5 replications. Variables measured were viability, motility and morphology of the treated fresh sperm stored for 14 days at temperature of l4oC. Based on the analysis of variance, the treatments had significant effect (P<0.05) on viability, motility and morphology of the sperm. In terms of viability, Pl(64.07%+-0.45) it was higher than control (P0=26.71%+-0.54), but it was not different for P2(57.44%+-0.52), P3(49.49%+-0.71), and P4 (47.83%+-0.61). The progress and very progress motility of spermatozoa were only found in control, and not in others. Non-progressive spermatozoa proportion of Pl, P2, P3 and P4,was found respectively. They were significantly higher than control.Forthe morphology, P4 had lower normal spermatozoa percentage than P0, Pl, P2 and P3, rvhereas among P0, P l, P2, and P3, there were not different (P>0,05).
first_indexed 2024-03-13T23:50:22Z
format Article
id oai:generic.eprints.org:273136
institution Universiti Gadjah Mada
language English
last_indexed 2024-03-13T23:50:22Z
publishDate 2010
publisher Fakultas Peternakan Universitas Gadjah Mada
record_format dspace
spelling oai:generic.eprints.org:2731362017-03-01T05:58:07Z https://repository.ugm.ac.id/273136/ In vitro fresh sperm preparation for maintaining sperm viability at Storage temperature of l4oC using tannin supplementation of lamtoro leaves Mirajuddin, Mirajuddin Kustono, Kustono Ismaya, Ismaya Budiyanto, Agung Veterinary Sciences A research on in vitro fresh sperm preparation for maintaining sperm viability at storage temperature of l4 derajat C using tannin supplementation of lamtoro leaves (Leucaena leucocephala)had been carried out in both Physiology and Animal Reproduction Laboratory, Faculty of Animal Science, Universitas Gadjah Mada for fresh sperm preparation and sperm quality evaluation, during March to July of 2009.The experiment was designed using the Complete Random Design consisted of 5 treatments and 5 replications. Variables measured were viability, motility and morphology of the treated fresh sperm stored for 14 days at temperature of l4oC. Based on the analysis of variance, the treatments had significant effect (P<0.05) on viability, motility and morphology of the sperm. In terms of viability, Pl(64.07%+-0.45) it was higher than control (P0=26.71%+-0.54), but it was not different for P2(57.44%+-0.52), P3(49.49%+-0.71), and P4 (47.83%+-0.61). The progress and very progress motility of spermatozoa were only found in control, and not in others. Non-progressive spermatozoa proportion of Pl, P2, P3 and P4,was found respectively. They were significantly higher than control.Forthe morphology, P4 had lower normal spermatozoa percentage than P0, Pl, P2 and P3, rvhereas among P0, P l, P2, and P3, there were not different (P>0,05). Fakultas Peternakan Universitas Gadjah Mada 2010 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/273136/1/1.%205%20th%20ISTAP%20International%20Seminar%20On%20Tropical%20Animal%20Production%20Proceedings%20Part%202.pdf Mirajuddin, Mirajuddin and Kustono, Kustono and Ismaya, Ismaya and Budiyanto, Agung (2010) In vitro fresh sperm preparation for maintaining sperm viability at Storage temperature of l4oC using tannin supplementation of lamtoro leaves. Proceedings Part 2 "Community Empowerment and Tropical Animal Industry. pp. 565-571. ISSN 978-979-1215-21-3
spellingShingle Veterinary Sciences
Mirajuddin, Mirajuddin
Kustono, Kustono
Ismaya, Ismaya
Budiyanto, Agung
In vitro fresh sperm preparation for maintaining sperm viability at Storage temperature of l4oC using tannin supplementation of lamtoro leaves
title In vitro fresh sperm preparation for maintaining sperm viability at Storage temperature of l4oC using tannin supplementation of lamtoro leaves
title_full In vitro fresh sperm preparation for maintaining sperm viability at Storage temperature of l4oC using tannin supplementation of lamtoro leaves
title_fullStr In vitro fresh sperm preparation for maintaining sperm viability at Storage temperature of l4oC using tannin supplementation of lamtoro leaves
title_full_unstemmed In vitro fresh sperm preparation for maintaining sperm viability at Storage temperature of l4oC using tannin supplementation of lamtoro leaves
title_short In vitro fresh sperm preparation for maintaining sperm viability at Storage temperature of l4oC using tannin supplementation of lamtoro leaves
title_sort in vitro fresh sperm preparation for maintaining sperm viability at storage temperature of l4oc using tannin supplementation of lamtoro leaves
topic Veterinary Sciences
url https://repository.ugm.ac.id/273136/1/1.%205%20th%20ISTAP%20International%20Seminar%20On%20Tropical%20Animal%20Production%20Proceedings%20Part%202.pdf
work_keys_str_mv AT mirajuddinmirajuddin invitrofreshspermpreparationformaintainingspermviabilityatstoragetemperatureofl4ocusingtanninsupplementationoflamtoroleaves
AT kustonokustono invitrofreshspermpreparationformaintainingspermviabilityatstoragetemperatureofl4ocusingtanninsupplementationoflamtoroleaves
AT ismayaismaya invitrofreshspermpreparationformaintainingspermviabilityatstoragetemperatureofl4ocusingtanninsupplementationoflamtoroleaves
AT budiyantoagung invitrofreshspermpreparationformaintainingspermviabilityatstoragetemperatureofl4ocusingtanninsupplementationoflamtoroleaves