The Effect of Narrow and Broad Band Ultraviolet B Onto Keloid Fibroblast-VEGF Expressions
ABSTRACT Yohanes Widodo Wirohadidjojo' & Ishandono Dahlan - The effect of narrow and broad band ultraviolet B onto keloid fibroblast- VEGF expressions Background: Collagenase inactivation of keloid lesions is due to plasminogen activator inhibitors which are synthesized under stimulation of...
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[Yogyakarta] : Universitas Gadjah Mada
2007
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| _version_ | 1797019665896046592 |
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| author | Perpustakaan UGM, i-lib |
| author_facet | Perpustakaan UGM, i-lib |
| author_sort | Perpustakaan UGM, i-lib |
| collection | UGM |
| description | ABSTRACT
Yohanes Widodo Wirohadidjojo' & Ishandono Dahlan - The effect of narrow and broad band ultraviolet B onto keloid fibroblast- VEGF expressions
Background: Collagenase inactivation of keloid lesions is due to plasminogen activator inhibitors which are synthesized under stimulation of vascular endothelial growth factor (VEGF), a released protein under hypoxia conditions. On the other hand, ultraviolet B (UVB) may generate various oxidative molecules of irradiated chromophores. The effect of UVB in VEGF synthesis is unclear.
ObJective. To know the effect of narrow and broad band ultraviolet-B on keloid fibroblast-VEGF expression. Materials and methods: Materials in this study were keloid materials collected from dermatosurgery and plastic surgery keloid revision. A parallel simple experiment study was performed to compare the effect of 0, 50, 75, and 100mJ/cm2 of broad band (BBUVB) as well as narrow band UVB (NBUVB) in VEGF synthesis of passage-3 keloid fibroblasts isolated from 4 patients. Samples were stained with monoclonal antibody anti VEGF. The selected DAB-brown colors of cytoplasm were computed based on Photoshop software histogram.
Results: Compared to untreated group, all of various NBUVB showed a very significant (P0.05) between NBUVB and BBUVB groups.
Conclusion: NBUVB as well as BBUVB can suppress VEGF synthesis among irradiated keloid fibroblasts. The 50 mJ/cm2 of NB UVB as well as 75 mJ/cm2 of BBUVB may be developed as the modality in keloid prevention or treatment.
Keywords: keloid-fibroblasts, broad-band UVB, narrow-band UVB, VEGF |
| first_indexed | 2024-03-05T23:10:46Z |
| format | Article |
| id | oai:generic.eprints.org:27319 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T19:03:11Z |
| publishDate | 2007 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:273192014-06-18T00:25:14Z https://repository.ugm.ac.id/27319/ The Effect of Narrow and Broad Band Ultraviolet B Onto Keloid Fibroblast-VEGF Expressions Perpustakaan UGM, i-lib Jurnal i-lib UGM ABSTRACT Yohanes Widodo Wirohadidjojo' & Ishandono Dahlan - The effect of narrow and broad band ultraviolet B onto keloid fibroblast- VEGF expressions Background: Collagenase inactivation of keloid lesions is due to plasminogen activator inhibitors which are synthesized under stimulation of vascular endothelial growth factor (VEGF), a released protein under hypoxia conditions. On the other hand, ultraviolet B (UVB) may generate various oxidative molecules of irradiated chromophores. The effect of UVB in VEGF synthesis is unclear. ObJective. To know the effect of narrow and broad band ultraviolet-B on keloid fibroblast-VEGF expression. Materials and methods: Materials in this study were keloid materials collected from dermatosurgery and plastic surgery keloid revision. A parallel simple experiment study was performed to compare the effect of 0, 50, 75, and 100mJ/cm2 of broad band (BBUVB) as well as narrow band UVB (NBUVB) in VEGF synthesis of passage-3 keloid fibroblasts isolated from 4 patients. Samples were stained with monoclonal antibody anti VEGF. The selected DAB-brown colors of cytoplasm were computed based on Photoshop software histogram. Results: Compared to untreated group, all of various NBUVB showed a very significant (P0.05) between NBUVB and BBUVB groups. Conclusion: NBUVB as well as BBUVB can suppress VEGF synthesis among irradiated keloid fibroblasts. The 50 mJ/cm2 of NB UVB as well as 75 mJ/cm2 of BBUVB may be developed as the modality in keloid prevention or treatment. Keywords: keloid-fibroblasts, broad-band UVB, narrow-band UVB, VEGF [Yogyakarta] : Universitas Gadjah Mada 2007 Article NonPeerReviewed Perpustakaan UGM, i-lib (2007) The Effect of Narrow and Broad Band Ultraviolet B Onto Keloid Fibroblast-VEGF Expressions. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=10372 |
| spellingShingle | Jurnal i-lib UGM Perpustakaan UGM, i-lib The Effect of Narrow and Broad Band Ultraviolet B Onto Keloid Fibroblast-VEGF Expressions |
| title | The Effect of Narrow and Broad Band Ultraviolet B Onto Keloid Fibroblast-VEGF Expressions |
| title_full | The Effect of Narrow and Broad Band Ultraviolet B Onto Keloid Fibroblast-VEGF Expressions |
| title_fullStr | The Effect of Narrow and Broad Band Ultraviolet B Onto Keloid Fibroblast-VEGF Expressions |
| title_full_unstemmed | The Effect of Narrow and Broad Band Ultraviolet B Onto Keloid Fibroblast-VEGF Expressions |
| title_short | The Effect of Narrow and Broad Band Ultraviolet B Onto Keloid Fibroblast-VEGF Expressions |
| title_sort | effect of narrow and broad band ultraviolet b onto keloid fibroblast vegf expressions |
| topic | Jurnal i-lib UGM |
| work_keys_str_mv | AT perpustakaanugmilib theeffectofnarrowandbroadbandultravioletbontokeloidfibroblastvegfexpressions AT perpustakaanugmilib effectofnarrowandbroadbandultravioletbontokeloidfibroblastvegfexpressions |