The Effect of Topical Extravirgin Olive Oil on Ultraviolet B-Induced 8-Hydroxy-2'-Deoxyguanosine Formation in Human Skin
ABSTRACT Catharlna Saglta, Yohanes Wldodo Wlrohadldjojo. Arlef Budlyanto. Irlanlwatl . The effect of topical extra virgin oil In UVB induced-B-hydroxy-2'-deoxyguanosine formation in human skin Background: Ultraviolet B (UVBI may cause DNA damage due to photooxydative reaction and form 8hydroxy-...
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[Yogyakarta] : Universitas Gadjah Mada
2007
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| _version_ | 1826033009198891008 |
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| author | Perpustakaan UGM, i-lib |
| author_facet | Perpustakaan UGM, i-lib |
| author_sort | Perpustakaan UGM, i-lib |
| collection | UGM |
| description | ABSTRACT
Catharlna Saglta, Yohanes Wldodo Wlrohadldjojo. Arlef Budlyanto. Irlanlwatl . The effect of topical extra virgin oil In UVB induced-B-hydroxy-2'-deoxyguanosine formation in human skin
Background: Ultraviolet B (UVBI may cause DNA damage due to photooxydative reaction and form 8hydroxy-2'-deoxyguanosine (8-0HdGI as a genotoxic product. Topically extra virgin olive oil (EVOOI can decrease 8-0HdG formation among UVB irradiated-hairless mice epidermal cells. Such effect in human skin has never been discovered.
Objective: To know the effect of topically EVOO in UVB induced-8-0HdG formation in human epidermal cells.
Method: A simple experiment was performed on cultured human foreskins. The foreskins were divided into 3 groups: group was treated with 100mJ/cm2 UVB + topical EVOO, group B with 100mJ/cm2 UVB, and group C as control. All skin was cultured in completed DMEM and fixation was performed at one and 24 hours. 8-0HdG expression was determined immunohistochemically with antibody anti 8-0HdG and measured based on histogram of selected colour of Adobe photoshop.
Results: The mean of 8-0HdG expression in experimental groups: (8.02:t 13.81, (26.95:t 23.231. and 14.96:t 7.141, respectively (p =0.0001 at an hour |
| first_indexed | 2024-03-13T19:03:11Z |
| format | Article |
| id | oai:generic.eprints.org:27320 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T19:03:11Z |
| publishDate | 2007 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:273202014-06-18T00:25:14Z https://repository.ugm.ac.id/27320/ The Effect of Topical Extravirgin Olive Oil on Ultraviolet B-Induced 8-Hydroxy-2'-Deoxyguanosine Formation in Human Skin Perpustakaan UGM, i-lib Jurnal i-lib UGM ABSTRACT Catharlna Saglta, Yohanes Wldodo Wlrohadldjojo. Arlef Budlyanto. Irlanlwatl . The effect of topical extra virgin oil In UVB induced-B-hydroxy-2'-deoxyguanosine formation in human skin Background: Ultraviolet B (UVBI may cause DNA damage due to photooxydative reaction and form 8hydroxy-2'-deoxyguanosine (8-0HdGI as a genotoxic product. Topically extra virgin olive oil (EVOOI can decrease 8-0HdG formation among UVB irradiated-hairless mice epidermal cells. Such effect in human skin has never been discovered. Objective: To know the effect of topically EVOO in UVB induced-8-0HdG formation in human epidermal cells. Method: A simple experiment was performed on cultured human foreskins. The foreskins were divided into 3 groups: group was treated with 100mJ/cm2 UVB + topical EVOO, group B with 100mJ/cm2 UVB, and group C as control. All skin was cultured in completed DMEM and fixation was performed at one and 24 hours. 8-0HdG expression was determined immunohistochemically with antibody anti 8-0HdG and measured based on histogram of selected colour of Adobe photoshop. Results: The mean of 8-0HdG expression in experimental groups: (8.02:t 13.81, (26.95:t 23.231. and 14.96:t 7.141, respectively (p =0.0001 at an hour [Yogyakarta] : Universitas Gadjah Mada 2007 Article NonPeerReviewed Perpustakaan UGM, i-lib (2007) The Effect of Topical Extravirgin Olive Oil on Ultraviolet B-Induced 8-Hydroxy-2'-Deoxyguanosine Formation in Human Skin. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=10374 |
| spellingShingle | Jurnal i-lib UGM Perpustakaan UGM, i-lib The Effect of Topical Extravirgin Olive Oil on Ultraviolet B-Induced 8-Hydroxy-2'-Deoxyguanosine Formation in Human Skin |
| title | The Effect of Topical Extravirgin Olive Oil on Ultraviolet B-Induced 8-Hydroxy-2'-Deoxyguanosine Formation in Human Skin |
| title_full | The Effect of Topical Extravirgin Olive Oil on Ultraviolet B-Induced 8-Hydroxy-2'-Deoxyguanosine Formation in Human Skin |
| title_fullStr | The Effect of Topical Extravirgin Olive Oil on Ultraviolet B-Induced 8-Hydroxy-2'-Deoxyguanosine Formation in Human Skin |
| title_full_unstemmed | The Effect of Topical Extravirgin Olive Oil on Ultraviolet B-Induced 8-Hydroxy-2'-Deoxyguanosine Formation in Human Skin |
| title_short | The Effect of Topical Extravirgin Olive Oil on Ultraviolet B-Induced 8-Hydroxy-2'-Deoxyguanosine Formation in Human Skin |
| title_sort | effect of topical extravirgin olive oil on ultraviolet b induced 8 hydroxy 2 deoxyguanosine formation in human skin |
| topic | Jurnal i-lib UGM |
| work_keys_str_mv | AT perpustakaanugmilib theeffectoftopicalextravirginoliveoilonultravioletbinduced8hydroxy2deoxyguanosineformationinhumanskin AT perpustakaanugmilib effectoftopicalextravirginoliveoilonultravioletbinduced8hydroxy2deoxyguanosineformationinhumanskin |