Localization and Molecular Size of Mucin2 Glycoproteins Forming the Gut Mucosal Barrier in the Indonesian Indigenous Naked Neck and Normal Feathered Chickens
The cecum of chicken gut may be more susceptible to pathogens because of colonization of microbes. Lower segment of gut is also the primary tissue where microorganisms may invade from external environment colonizing in the cloaca. Mucin composed of glycoproteins play significant roles in the barrier...
| Main Authors: | , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
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2015
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| Online Access: | https://repository.ugm.ac.id/273644/1/ISTAP_BambangAriyadi.pdf |
| _version_ | 1826049737240870912 |
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| author | Ariyadi, Bambang Sidadolog, Jafendi Hasoloan Purba Harimurti, Sri Sudaryati, Sri Wihandoyo, Wihandoyo |
| author_facet | Ariyadi, Bambang Sidadolog, Jafendi Hasoloan Purba Harimurti, Sri Sudaryati, Sri Wihandoyo, Wihandoyo |
| author_sort | Ariyadi, Bambang |
| collection | UGM |
| description | The cecum of chicken gut may be more susceptible to pathogens because of colonization of microbes. Lower segment of gut is also the primary tissue where microorganisms may invade from external environment colonizing in the cloaca. Mucin composed of glycoproteins play significant roles in the barrier against infection on the mucosal surface. The aim of this study was to determine the differences in the mucosal barrier formation in the lower segment of gut between Indonesian naked neck and normal feathered chickens. The lower segments of gut (rectum, colon, and cecal tonsil) of Indonesian indigenous naked neck and normal chickens were collected. The expression of mucin2 gene in the gut mucosa was analyzed by reverse-transcription-polymerase chain reaction (RT-PCR). Localization and molecular size of the mucosal glycoproteins were analyzed by western blot methods. WGA and Jacalin lectins were used for western blot analysis. Mucin-2 gene was expressed in mucosal gut of rectum, colon, and cecal tonsil in both naked neck and normal chickens. Western blot analysis showed single band in both WGA and Jacalin in mucosal gut of rectum, colon, and cecal tonsil in both naked neck and normal chickens. These results suggest that mucin2 gene as well as glycoprotein containing WGA and Jacalin positive sugars covers the surface of mucosal gut in both naked neck and normal chickens, probably to form mucosal barrier.
Keywords: Indonesian naked neck chickens, Mucosal gut, Mucin-2, Glycoprotein. |
| first_indexed | 2024-03-13T23:51:18Z |
| format | Conference or Workshop Item |
| id | oai:generic.eprints.org:273644 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-13T23:51:18Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2736442017-07-31T04:31:46Z https://repository.ugm.ac.id/273644/ Localization and Molecular Size of Mucin2 Glycoproteins Forming the Gut Mucosal Barrier in the Indonesian Indigenous Naked Neck and Normal Feathered Chickens Ariyadi, Bambang Sidadolog, Jafendi Hasoloan Purba Harimurti, Sri Sudaryati, Sri Wihandoyo, Wihandoyo Biochemistry and Cell Biology Cell Metabolism Gene Expression (incl. Microarray and other genome-wide approaches) Animal Physiology - Cell Animal Physiology - Systems Animal Protection The cecum of chicken gut may be more susceptible to pathogens because of colonization of microbes. Lower segment of gut is also the primary tissue where microorganisms may invade from external environment colonizing in the cloaca. Mucin composed of glycoproteins play significant roles in the barrier against infection on the mucosal surface. The aim of this study was to determine the differences in the mucosal barrier formation in the lower segment of gut between Indonesian naked neck and normal feathered chickens. The lower segments of gut (rectum, colon, and cecal tonsil) of Indonesian indigenous naked neck and normal chickens were collected. The expression of mucin2 gene in the gut mucosa was analyzed by reverse-transcription-polymerase chain reaction (RT-PCR). Localization and molecular size of the mucosal glycoproteins were analyzed by western blot methods. WGA and Jacalin lectins were used for western blot analysis. Mucin-2 gene was expressed in mucosal gut of rectum, colon, and cecal tonsil in both naked neck and normal chickens. Western blot analysis showed single band in both WGA and Jacalin in mucosal gut of rectum, colon, and cecal tonsil in both naked neck and normal chickens. These results suggest that mucin2 gene as well as glycoprotein containing WGA and Jacalin positive sugars covers the surface of mucosal gut in both naked neck and normal chickens, probably to form mucosal barrier. Keywords: Indonesian naked neck chickens, Mucosal gut, Mucin-2, Glycoprotein. 2015-10-20 Conference or Workshop Item NonPeerReviewed application/pdf en https://repository.ugm.ac.id/273644/1/ISTAP_BambangAriyadi.pdf Ariyadi, Bambang and Sidadolog, Jafendi Hasoloan Purba and Harimurti, Sri and Sudaryati, Sri and Wihandoyo, Wihandoyo (2015) Localization and Molecular Size of Mucin2 Glycoproteins Forming the Gut Mucosal Barrier in the Indonesian Indigenous Naked Neck and Normal Feathered Chickens. In: The 6th International Seminar on Tropical Animal Production, October 20-22, 2015, Faculty of Animal Science, Universitas Gadjah Mada Yogyakarta, Indonesia. |
| spellingShingle | Biochemistry and Cell Biology Cell Metabolism Gene Expression (incl. Microarray and other genome-wide approaches) Animal Physiology - Cell Animal Physiology - Systems Animal Protection Ariyadi, Bambang Sidadolog, Jafendi Hasoloan Purba Harimurti, Sri Sudaryati, Sri Wihandoyo, Wihandoyo Localization and Molecular Size of Mucin2 Glycoproteins Forming the Gut Mucosal Barrier in the Indonesian Indigenous Naked Neck and Normal Feathered Chickens |
| title | Localization and Molecular Size of Mucin2 Glycoproteins Forming the Gut Mucosal Barrier in the Indonesian Indigenous Naked Neck and Normal Feathered Chickens |
| title_full | Localization and Molecular Size of Mucin2 Glycoproteins Forming the Gut Mucosal Barrier in the Indonesian Indigenous Naked Neck and Normal Feathered Chickens |
| title_fullStr | Localization and Molecular Size of Mucin2 Glycoproteins Forming the Gut Mucosal Barrier in the Indonesian Indigenous Naked Neck and Normal Feathered Chickens |
| title_full_unstemmed | Localization and Molecular Size of Mucin2 Glycoproteins Forming the Gut Mucosal Barrier in the Indonesian Indigenous Naked Neck and Normal Feathered Chickens |
| title_short | Localization and Molecular Size of Mucin2 Glycoproteins Forming the Gut Mucosal Barrier in the Indonesian Indigenous Naked Neck and Normal Feathered Chickens |
| title_sort | localization and molecular size of mucin2 glycoproteins forming the gut mucosal barrier in the indonesian indigenous naked neck and normal feathered chickens |
| topic | Biochemistry and Cell Biology Cell Metabolism Gene Expression (incl. Microarray and other genome-wide approaches) Animal Physiology - Cell Animal Physiology - Systems Animal Protection |
| url | https://repository.ugm.ac.id/273644/1/ISTAP_BambangAriyadi.pdf |
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