Feeding Strategy of Ruminants and Its Potential Effect on Methane Emission Reduction

A study was conducted to determine the potential effect of Leucaena leucocephalain the diet with 3 levels 0%, 6%, 12% of ration on the population of rumen methanogenic bacteria of cattle and buffalo. Three each ruminally-fistulated (body weight 342 ± 66.93 kg) were used in this experiment. The amou...

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Main Authors: Suwignyo, Bambang, Suhartanto, Bambang, Umami, Nafiatul, Suseno, Nilo, Bachruddin, Zaenal
Format: Article
Language:English
Published: Canadian Center of Science and Education 2016
Subjects:
Online Access:https://repository.ugm.ac.id/273658/1/JAS_Feeding%20strategy%20of%20Ruminants%20and%20Its%20Potential%20Effect%20on%20Methane%20Emission%20Reduction.pdf
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author Suwignyo, Bambang
Suhartanto, Bambang
Umami, Nafiatul
Suseno, Nilo
Bachruddin, Zaenal
author_facet Suwignyo, Bambang
Suhartanto, Bambang
Umami, Nafiatul
Suseno, Nilo
Bachruddin, Zaenal
author_sort Suwignyo, Bambang
collection UGM
description A study was conducted to determine the potential effect of Leucaena leucocephalain the diet with 3 levels 0%, 6%, 12% of ration on the population of rumen methanogenic bacteria of cattle and buffalo. Three each ruminally-fistulated (body weight 342 ± 66.93 kg) were used in this experiment. The amount of feed offered was 2.5% of live weight on DM basis. Rumen fluid was collected from each animal before feeding, after 17 days on feed. The rumen fluid was strained it through cheesecloth and stored in freezer prior to analysis. The samples were subjected to DNA extraction and amplification. Three universal primers were used to detect methanogenic bacteria, which had more than one band, ranging from 500 bp and 1.4 kbp. The results indicated that the level of Leucaena leucocephala in the diets reduced the population of methanogenic rumen bacteria of the cattle and enhanced the Fibrobacter succinogenes. Thus, reduction of methane production increases rumen propionate since methane production is inversely proportional with propionate production. Leucaena leucocephala give many benefit e.g. for ruminant that will have a good impact in the term of ruminant nutrition and global environmental contribution through reducing methanogens in the rumen
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spelling oai:generic.eprints.org:2736582017-08-02T06:09:01Z https://repository.ugm.ac.id/273658/ Feeding Strategy of Ruminants and Its Potential Effect on Methane Emission Reduction Suwignyo, Bambang Suhartanto, Bambang Umami, Nafiatul Suseno, Nilo Bachruddin, Zaenal Genetics Biological Sciences Livestock A study was conducted to determine the potential effect of Leucaena leucocephalain the diet with 3 levels 0%, 6%, 12% of ration on the population of rumen methanogenic bacteria of cattle and buffalo. Three each ruminally-fistulated (body weight 342 ± 66.93 kg) were used in this experiment. The amount of feed offered was 2.5% of live weight on DM basis. Rumen fluid was collected from each animal before feeding, after 17 days on feed. The rumen fluid was strained it through cheesecloth and stored in freezer prior to analysis. The samples were subjected to DNA extraction and amplification. Three universal primers were used to detect methanogenic bacteria, which had more than one band, ranging from 500 bp and 1.4 kbp. The results indicated that the level of Leucaena leucocephala in the diets reduced the population of methanogenic rumen bacteria of the cattle and enhanced the Fibrobacter succinogenes. Thus, reduction of methane production increases rumen propionate since methane production is inversely proportional with propionate production. Leucaena leucocephala give many benefit e.g. for ruminant that will have a good impact in the term of ruminant nutrition and global environmental contribution through reducing methanogens in the rumen Canadian Center of Science and Education 2016-08-15 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/273658/1/JAS_Feeding%20strategy%20of%20Ruminants%20and%20Its%20Potential%20Effect%20on%20Methane%20Emission%20Reduction.pdf Suwignyo, Bambang and Suhartanto, Bambang and Umami, Nafiatul and Suseno, Nilo and Bachruddin, Zaenal (2016) Feeding Strategy of Ruminants and Its Potential Effect on Methane Emission Reduction. Journal of Agricultural Science, 8 (9). pp. 199-204. ISSN 1916-9752 http://www.ccsenet.org/journal/index.php/jas
spellingShingle Genetics
Biological Sciences
Livestock
Suwignyo, Bambang
Suhartanto, Bambang
Umami, Nafiatul
Suseno, Nilo
Bachruddin, Zaenal
Feeding Strategy of Ruminants and Its Potential Effect on Methane Emission Reduction
title Feeding Strategy of Ruminants and Its Potential Effect on Methane Emission Reduction
title_full Feeding Strategy of Ruminants and Its Potential Effect on Methane Emission Reduction
title_fullStr Feeding Strategy of Ruminants and Its Potential Effect on Methane Emission Reduction
title_full_unstemmed Feeding Strategy of Ruminants and Its Potential Effect on Methane Emission Reduction
title_short Feeding Strategy of Ruminants and Its Potential Effect on Methane Emission Reduction
title_sort feeding strategy of ruminants and its potential effect on methane emission reduction
topic Genetics
Biological Sciences
Livestock
url https://repository.ugm.ac.id/273658/1/JAS_Feeding%20strategy%20of%20Ruminants%20and%20Its%20Potential%20Effect%20on%20Methane%20Emission%20Reduction.pdf
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AT suhartantobambang feedingstrategyofruminantsanditspotentialeffectonmethaneemissionreduction
AT umaminafiatul feedingstrategyofruminantsanditspotentialeffectonmethaneemissionreduction
AT susenonilo feedingstrategyofruminantsanditspotentialeffectonmethaneemissionreduction
AT bachruddinzaenal feedingstrategyofruminantsanditspotentialeffectonmethaneemissionreduction