Expression of tight junction molecule “claudins” in the lower oviductal segments and their changes with egg-laying phase and gonadal steroid stimulation in hens.
Tight junctions in the mucosal epithelium have essential roles as a mucosal barrier to prevent invasion of microbes into the hen oviduct tissue. The aim of this study was to determine the effects of the egg-laying phase and estradiol on the expression of tight junction molecule “claudins” in the low...
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| Format: | Article |
| Language: | English |
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Elsevier B.V.
2013
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| Online Access: | https://repository.ugm.ac.id/273763/1/Ariyadi%20et%20al.Therio.2013.79.no2.pdf |
| _version_ | 1797036774869958656 |
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| author | Ariyadi, Bambang Yoshimura, Yukinori Isobe, Naoki |
| author_facet | Ariyadi, Bambang Yoshimura, Yukinori Isobe, Naoki |
| author_sort | Ariyadi, Bambang |
| collection | UGM |
| description | Tight junctions in the mucosal epithelium have essential roles as a mucosal barrier to prevent invasion of microbes into the hen oviduct tissue. The aim of this study was to determine the effects of the egg-laying phase and estradiol on the expression of tight junction molecule “claudins” in the lower oviductal segments in hens. White Leghorn laying and molting hens were used. Molting hens were given either sesame oil (vehicle) or estradiol benzoate (N ¼ 5 per group) via injection. The lower segments of oviduct (isthmus, uterus, and vagina) of these birds were collected. Gene expression of claudin-1, -3, -5, lipopolysaccharide-induced TNFa factor (LITAF), and IFNˠ was analyzed by quantitative reverse transcription polymerase chain reaction, and localization of claudin-1 was examined by immunohistochemistry. Permeability in the mucosal epithelium was assessed by intrauterine injection of fluorescein isothiocyanate-dextran. Expression of claudin-1, -3, and -5 genes and density of claudin-1 protein in the lower oviductal segments were higher in laying hens than in molting hens (P < 0.01); their expression was upregulated by estradiol (P< 0.01). Expression of LITAF and IFNˠ geneswas higher in molting hens than in laying hens |
| first_indexed | 2024-03-13T23:51:33Z |
| format | Article |
| id | oai:generic.eprints.org:273763 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-13T23:51:33Z |
| publishDate | 2013 |
| publisher | Elsevier B.V. |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2737632017-09-11T06:05:00Z https://repository.ugm.ac.id/273763/ Expression of tight junction molecule “claudins” in the lower oviductal segments and their changes with egg-laying phase and gonadal steroid stimulation in hens. Ariyadi, Bambang Yoshimura, Yukinori Isobe, Naoki Animal Production Animal Reproduction Tight junctions in the mucosal epithelium have essential roles as a mucosal barrier to prevent invasion of microbes into the hen oviduct tissue. The aim of this study was to determine the effects of the egg-laying phase and estradiol on the expression of tight junction molecule “claudins” in the lower oviductal segments in hens. White Leghorn laying and molting hens were used. Molting hens were given either sesame oil (vehicle) or estradiol benzoate (N ¼ 5 per group) via injection. The lower segments of oviduct (isthmus, uterus, and vagina) of these birds were collected. Gene expression of claudin-1, -3, -5, lipopolysaccharide-induced TNFa factor (LITAF), and IFNˠ was analyzed by quantitative reverse transcription polymerase chain reaction, and localization of claudin-1 was examined by immunohistochemistry. Permeability in the mucosal epithelium was assessed by intrauterine injection of fluorescein isothiocyanate-dextran. Expression of claudin-1, -3, and -5 genes and density of claudin-1 protein in the lower oviductal segments were higher in laying hens than in molting hens (P < 0.01); their expression was upregulated by estradiol (P< 0.01). Expression of LITAF and IFNˠ geneswas higher in molting hens than in laying hens Elsevier B.V. 2013 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/273763/1/Ariyadi%20et%20al.Therio.2013.79.no2.pdf Ariyadi, Bambang and Yoshimura, Yukinori and Isobe, Naoki (2013) Expression of tight junction molecule “claudins” in the lower oviductal segments and their changes with egg-laying phase and gonadal steroid stimulation in hens. Theriogenology., 79 (1). pp. 211-218. ISSN 0093-691X http://www.theriojournal.com/ |
| spellingShingle | Animal Production Animal Reproduction Ariyadi, Bambang Yoshimura, Yukinori Isobe, Naoki Expression of tight junction molecule “claudins” in the lower oviductal segments and their changes with egg-laying phase and gonadal steroid stimulation in hens. |
| title | Expression of tight junction molecule “claudins” in the lower oviductal segments and their changes with egg-laying phase and gonadal steroid stimulation in hens. |
| title_full | Expression of tight junction molecule “claudins” in the lower oviductal segments and their changes with egg-laying phase and gonadal steroid stimulation in hens. |
| title_fullStr | Expression of tight junction molecule “claudins” in the lower oviductal segments and their changes with egg-laying phase and gonadal steroid stimulation in hens. |
| title_full_unstemmed | Expression of tight junction molecule “claudins” in the lower oviductal segments and their changes with egg-laying phase and gonadal steroid stimulation in hens. |
| title_short | Expression of tight junction molecule “claudins” in the lower oviductal segments and their changes with egg-laying phase and gonadal steroid stimulation in hens. |
| title_sort | expression of tight junction molecule claudins in the lower oviductal segments and their changes with egg laying phase and gonadal steroid stimulation in hens |
| topic | Animal Production Animal Reproduction |
| url | https://repository.ugm.ac.id/273763/1/Ariyadi%20et%20al.Therio.2013.79.no2.pdf |
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