Kajian Molekular Tarsius sp. Pada Gen Penyandi Cytochrome Oxidase Subunit 2 Mitokondria The Molecular Study on Mitochondrial Cytochrome Oxidase 2(COX2) Gene of Tarsius sp.

Tarsius is an endemic species in Indonesia that is endangered. In-situ and ex-situ conservation of this species would yield better results if this genetic make up and diversity is determined. The objective of this ressearch was to study the specific genetic marker on COX2 gene of Tarsius sp. Sequenc...

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Main Authors:	WIDAYANTI, RINI, Handayani, Niken Satuti Nur, Budiarsa, I. Made
Format:	Article
Language:	English
Published:	Fak. Teknobiologi UAJY, Perhimpunan Biologi Indonesia, Solaris cell club dan PT. Digital Mobile File 2010
Subjects:	Veterinary Sciences
Online Access:	https://repository.ugm.ac.id/274005/1/Biota_Februari%202010.pdf

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author	WIDAYANTI, RINI Handayani, Niken Satuti Nur Budiarsa, I. Made
author_facet	WIDAYANTI, RINI Handayani, Niken Satuti Nur Budiarsa, I. Made
author_sort	WIDAYANTI, RINI
collection	UGM
description	Tarsius is an endemic species in Indonesia that is endangered. In-situ and ex-situ conservation of this species would yield better results if this genetic make up and diversity is determined. The objective of this ressearch was to study the specific genetic marker on COX2 gene of Tarsius sp. Sequencing of PCR product using primer COX2F and COX2R resulted in base sequence of 513 nts. Results of COX2 fragments sequencing were put on multiple alignment with other primates from Genbank with aid of software Clustal W, and were analyzed using MEGA program version 4.1. Eight different amino acid sites were found (amino acid no. 5, 6, 13, 14, 30, 35, 44 and 168). The genetic distance based on nucleotide COX2 calculated using Kimura 2-parameter model indicated that in the smallest genetic distance 0%, biggest 6.8% and average 2.3%. The phylogenetic tree using neighbor joining method based on the sequence of nucleotide and amino acid COX2 reveded differentiation among Tarsius from Lampung and Tarsius from Sulawesi, but could not be used to differentiate among T. dianae (from Central Sulawesi) and T. spectrum from North Sulawesi).
first_indexed	2024-03-13T23:51:58Z
format	Article
id	oai:generic.eprints.org:274005
institution	Universiti Gadjah Mada
language	English
last_indexed	2024-03-13T23:51:58Z
publishDate	2010
publisher	Fak. Teknobiologi UAJY, Perhimpunan Biologi Indonesia, Solaris cell club dan PT. Digital Mobile File
record_format	dspace
spelling	oai:generic.eprints.org:2740052018-01-09T05:06:29Z https://repository.ugm.ac.id/274005/ Kajian Molekular Tarsius sp. Pada Gen Penyandi Cytochrome Oxidase Subunit 2 Mitokondria The Molecular Study on Mitochondrial Cytochrome Oxidase 2(COX2) Gene of Tarsius sp. WIDAYANTI, RINI Handayani, Niken Satuti Nur Budiarsa, I. Made Veterinary Sciences Tarsius is an endemic species in Indonesia that is endangered. In-situ and ex-situ conservation of this species would yield better results if this genetic make up and diversity is determined. The objective of this ressearch was to study the specific genetic marker on COX2 gene of Tarsius sp. Sequencing of PCR product using primer COX2F and COX2R resulted in base sequence of 513 nts. Results of COX2 fragments sequencing were put on multiple alignment with other primates from Genbank with aid of software Clustal W, and were analyzed using MEGA program version 4.1. Eight different amino acid sites were found (amino acid no. 5, 6, 13, 14, 30, 35, 44 and 168). The genetic distance based on nucleotide COX2 calculated using Kimura 2-parameter model indicated that in the smallest genetic distance 0%, biggest 6.8% and average 2.3%. The phylogenetic tree using neighbor joining method based on the sequence of nucleotide and amino acid COX2 reveded differentiation among Tarsius from Lampung and Tarsius from Sulawesi, but could not be used to differentiate among T. dianae (from Central Sulawesi) and T. spectrum from North Sulawesi). Fak. Teknobiologi UAJY, Perhimpunan Biologi Indonesia, Solaris cell club dan PT. Digital Mobile File 2010-02-01 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/274005/1/Biota_Februari%202010.pdf WIDAYANTI, RINI and Handayani, Niken Satuti Nur and Budiarsa, I. Made (2010) Kajian Molekular Tarsius sp. Pada Gen Penyandi Cytochrome Oxidase Subunit 2 Mitokondria The Molecular Study on Mitochondrial Cytochrome Oxidase 2(COX2) Gene of Tarsius sp. Jurnal Biota, 15 (1). pp. 98-106. ISSN 0853-8670 http://www.uajy.ac.id/penelitian/jurnal/biota
spellingShingle	Veterinary Sciences WIDAYANTI, RINI Handayani, Niken Satuti Nur Budiarsa, I. Made Kajian Molekular Tarsius sp. Pada Gen Penyandi Cytochrome Oxidase Subunit 2 Mitokondria The Molecular Study on Mitochondrial Cytochrome Oxidase 2(COX2) Gene of Tarsius sp.
title	Kajian Molekular Tarsius sp. Pada Gen Penyandi Cytochrome Oxidase Subunit 2 Mitokondria The Molecular Study on Mitochondrial Cytochrome Oxidase 2(COX2) Gene of Tarsius sp.
title_full	Kajian Molekular Tarsius sp. Pada Gen Penyandi Cytochrome Oxidase Subunit 2 Mitokondria The Molecular Study on Mitochondrial Cytochrome Oxidase 2(COX2) Gene of Tarsius sp.
title_fullStr	Kajian Molekular Tarsius sp. Pada Gen Penyandi Cytochrome Oxidase Subunit 2 Mitokondria The Molecular Study on Mitochondrial Cytochrome Oxidase 2(COX2) Gene of Tarsius sp.
title_full_unstemmed	Kajian Molekular Tarsius sp. Pada Gen Penyandi Cytochrome Oxidase Subunit 2 Mitokondria The Molecular Study on Mitochondrial Cytochrome Oxidase 2(COX2) Gene of Tarsius sp.
title_short	Kajian Molekular Tarsius sp. Pada Gen Penyandi Cytochrome Oxidase Subunit 2 Mitokondria The Molecular Study on Mitochondrial Cytochrome Oxidase 2(COX2) Gene of Tarsius sp.
title_sort	kajian molekular tarsius sp pada gen penyandi cytochrome oxidase subunit 2 mitokondria the molecular study on mitochondrial cytochrome oxidase 2 cox2 gene of tarsius sp
topic	Veterinary Sciences
url	https://repository.ugm.ac.id/274005/1/Biota_Februari%202010.pdf
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Kajian Molekular Tarsius sp. Pada Gen Penyandi Cytochrome Oxidase Subunit 2 Mitokondria The Molecular Study on Mitochondrial Cytochrome Oxidase 2(COX2) Gene of Tarsius sp.

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