Expression of DMRT1 In Qual Embryos (Coturnix cotunix japonica)

The sex differentiation in the quail is a complex process of formation and involves many genes. One of the genes suspected of having a major influence on genital formation is DMRTI.This study aimed to see the level of DMR TI gene expression ir. the quail embryo The sample of the study was...

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Main Authors: Kusumawati, Asmarani, Istitawati, Ninik, Aldora, Alice, Prihatno, Surya Agus, Gustari, Sri, Budiyanto, Agung
Format: Article
Language:English
Published: Fakultas Kedokteran Hewan, Universitas Gadjah Mada 2018
Subjects:
Online Access:https://repository.ugm.ac.id/274419/1/77%20Expression%20of%20DMRT1%20in%20Quail%20Embryos%20%2B%20sertifikat.pdf
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author Kusumawati, Asmarani
Istitawati, Ninik
Aldora, Alice
Prihatno, Surya Agus
Gustari, Sri
Budiyanto, Agung
author_facet Kusumawati, Asmarani
Istitawati, Ninik
Aldora, Alice
Prihatno, Surya Agus
Gustari, Sri
Budiyanto, Agung
author_sort Kusumawati, Asmarani
collection UGM
description The sex differentiation in the quail is a complex process of formation and involves many genes. One of the genes suspected of having a major influence on genital formation is DMRTI.This study aimed to see the level of DMR TI gene expression ir. the quail embryo The sample of the study was the gonadal tissue of quai l c:mbryos obtained from the fenile quai l eggs. Eggs ore incubated and mkcn daily to be isolated from the gonadal genetic matenaL Genetic material in the form of DNA was used for molecu lar sexing using CllDW gene. RNA was used to mcasurc the exprcss1on of DMRTI gene in quail embryo Measurement of gene expression on days 1-8 was confirmed by nnnodrop spectrophotometer The results show that DMRTI gene expression has differences in male and female embryos. DMRTI gene expression in male embryos ha. h ighl:r mtcnsiry than females. DJHRTI gene is also seen to have maximum expression in male embryo's agecl 3-5 days. Th1s DMRTI gene may have an unponant role in the process of genita l fonnation and differentiation of qunil embryos. especially in males.
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spelling oai:generic.eprints.org:2744192021-01-08T03:58:32Z https://repository.ugm.ac.id/274419/ Expression of DMRT1 In Qual Embryos (Coturnix cotunix japonica) Kusumawati, Asmarani Istitawati, Ninik Aldora, Alice Prihatno, Surya Agus Gustari, Sri Budiyanto, Agung Veterinary Sciences The sex differentiation in the quail is a complex process of formation and involves many genes. One of the genes suspected of having a major influence on genital formation is DMRTI.This study aimed to see the level of DMR TI gene expression ir. the quail embryo The sample of the study was the gonadal tissue of quai l c:mbryos obtained from the fenile quai l eggs. Eggs ore incubated and mkcn daily to be isolated from the gonadal genetic matenaL Genetic material in the form of DNA was used for molecu lar sexing using CllDW gene. RNA was used to mcasurc the exprcss1on of DMRTI gene in quail embryo Measurement of gene expression on days 1-8 was confirmed by nnnodrop spectrophotometer The results show that DMRTI gene expression has differences in male and female embryos. DMRTI gene expression in male embryos ha. h ighl:r mtcnsiry than females. DJHRTI gene is also seen to have maximum expression in male embryo's agecl 3-5 days. Th1s DMRTI gene may have an unponant role in the process of genita l fonnation and differentiation of qunil embryos. especially in males. Fakultas Kedokteran Hewan, Universitas Gadjah Mada 2018-02-28 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/274419/1/77%20Expression%20of%20DMRT1%20in%20Quail%20Embryos%20%2B%20sertifikat.pdf Kusumawati, Asmarani and Istitawati, Ninik and Aldora, Alice and Prihatno, Surya Agus and Gustari, Sri and Budiyanto, Agung (2018) Expression of DMRT1 In Qual Embryos (Coturnix cotunix japonica). Proceeding International Symposium in Veterinary Science: Strengthening the Regional Veterinary Education and Reseach for the Future Excellent Veterinary Graduates. ISSN 978-6027-368446
spellingShingle Veterinary Sciences
Kusumawati, Asmarani
Istitawati, Ninik
Aldora, Alice
Prihatno, Surya Agus
Gustari, Sri
Budiyanto, Agung
Expression of DMRT1 In Qual Embryos (Coturnix cotunix japonica)
title Expression of DMRT1 In Qual Embryos (Coturnix cotunix japonica)
title_full Expression of DMRT1 In Qual Embryos (Coturnix cotunix japonica)
title_fullStr Expression of DMRT1 In Qual Embryos (Coturnix cotunix japonica)
title_full_unstemmed Expression of DMRT1 In Qual Embryos (Coturnix cotunix japonica)
title_short Expression of DMRT1 In Qual Embryos (Coturnix cotunix japonica)
title_sort expression of dmrt1 in qual embryos coturnix cotunix japonica
topic Veterinary Sciences
url https://repository.ugm.ac.id/274419/1/77%20Expression%20of%20DMRT1%20in%20Quail%20Embryos%20%2B%20sertifikat.pdf
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